Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Abstract DNA metabarcoding is a powerful tool to assess arthropod diversity in environmental bulk samples such as Malaise trap, pitfall trap, or hand net samples. While comparative performance tests for different extraction protocols, primers, and Taq polymerases have been made, the effect of differ...

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Bibliographic Details
Main Authors: Dominik Buchner, Arne J. Beermann, Florian Leese, Martina Weiss
Format: Article
Language:English
Published: Wiley 2021-07-01
Series:Ecology and Evolution
Subjects:
amplicon sequencing
biomonitoring
bulk sample
Malaise trap
PCR bias
Online Access:https://doi.org/10.1002/ece3.7753

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