Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Abstract DNA metabarcoding is a powerful tool to assess arthropod diversity in environmental bulk samples such as Malaise trap, pitfall trap, or hand net samples. While comparative performance tests for different extraction protocols, primers, and Taq polymerases have been made, the effect of differ...

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Dades bibliogràfiques
Autors principals:	Dominik Buchner, Arne J. Beermann, Florian Leese, Martina Weiss
Format:	Article
Idioma:	English
Publicat:	Wiley 2021-07-01
Col·lecció:	Ecology and Evolution
Matèries:	amplicon sequencing biomonitoring bulk sample Malaise trap PCR bias
Accés en línia:	https://doi.org/10.1002/ece3.7753

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