Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Abstract DNA metabarcoding is a powerful tool to assess arthropod diversity in environmental bulk samples such as Malaise trap, pitfall trap, or hand net samples. While comparative performance tests for different extraction protocols, primers, and Taq polymerases have been made, the effect of differ...

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Detalles Bibliográficos
Autores principales:	Dominik Buchner, Arne J. Beermann, Florian Leese, Martina Weiss
Formato:	Artículo
Lenguaje:	English
Publicado:	Wiley 2021-07-01
Colección:	Ecology and Evolution
Materias:	amplicon sequencing biomonitoring bulk sample Malaise trap PCR bias
Acceso en línea:	https://doi.org/10.1002/ece3.7753

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