Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Abstract DNA metabarcoding is a powerful tool to assess arthropod diversity in environmental bulk samples such as Malaise trap, pitfall trap, or hand net samples. While comparative performance tests for different extraction protocols, primers, and Taq polymerases have been made, the effect of differ...

Cur síos iomlán

Sonraí bibleagrafaíochta
Príomhchruthaitheoirí:	Dominik Buchner, Arne J. Beermann, Florian Leese, Martina Weiss
Formáid:	Alt
Teanga:	English
Foilsithe / Cruthaithe:	Wiley 2021-07-01
Sraith:	Ecology and Evolution
Ábhair:	amplicon sequencing biomonitoring bulk sample Malaise trap PCR bias
Rochtain ar líne:	https://doi.org/10.1002/ece3.7753

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