Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Abstract DNA metabarcoding is a powerful tool to assess arthropod diversity in environmental bulk samples such as Malaise trap, pitfall trap, or hand net samples. While comparative performance tests for different extraction protocols, primers, and Taq polymerases have been made, the effect of differ...

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Dettagli Bibliografici
Autori principali:	Dominik Buchner, Arne J. Beermann, Florian Leese, Martina Weiss
Natura:	Articolo
Lingua:	English
Pubblicazione:	Wiley 2021-07-01
Serie:	Ecology and Evolution
Soggetti:	amplicon sequencing biomonitoring bulk sample Malaise trap PCR bias
Accesso online:	https://doi.org/10.1002/ece3.7753

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