Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Abstract DNA metabarcoding is a powerful tool to assess arthropod diversity in environmental bulk samples such as Malaise trap, pitfall trap, or hand net samples. While comparative performance tests for different extraction protocols, primers, and Taq polymerases have been made, the effect of differ...

書誌詳細
主要な著者:	Dominik Buchner, Arne J. Beermann, Florian Leese, Martina Weiss
フォーマット:	論文
言語:	English
出版事項:	Wiley 2021-07-01
シリーズ:	Ecology and Evolution
主題:	amplicon sequencing biomonitoring bulk sample Malaise trap PCR bias
オンライン･アクセス:	https://doi.org/10.1002/ece3.7753

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