Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Abstract DNA metabarcoding is a powerful tool to assess arthropod diversity in environmental bulk samples such as Malaise trap, pitfall trap, or hand net samples. While comparative performance tests for different extraction protocols, primers, and Taq polymerases have been made, the effect of differ...

Бүрэн тодорхойлолт

Номзүйн дэлгэрэнгүй
Үндсэн зохиолчид:	Dominik Buchner, Arne J. Beermann, Florian Leese, Martina Weiss
Формат:	Өгүүллэг
Хэл сонгох:	English
Хэвлэсэн:	Wiley 2021-07-01
Цуврал:	Ecology and Evolution
Нөхцлүүд:	amplicon sequencing biomonitoring bulk sample Malaise trap PCR bias
Онлайн хандалт:	https://doi.org/10.1002/ece3.7753

Ижил төстэй зүйлс

Maximizing Identification Precision of Hymenoptera and Brachycera (Diptera) With a Non‐Destructive DNA Metabarcoding Approach
-н: Isabel C. Kilian, зэрэг
Хэвлэсэн: (2025-01-01)

Is it worth the extra mile? Comparing environmental DNA and RNA metabarcoding for vertebrate and invertebrate biodiversity surveys in a lowland stream
-н: Till-Hendrik Macher, зэрэг
Хэвлэсэн: (2024-10-01)

Persisting roadblocks in arthropod monitoring using non-destructive metabarcoding from collection media of passive traps
-н: Lucas Sire, зэрэг
Хэвлэсэн: (2023-10-01)

Enhancement of PCRs by Partial Restriction Digestion of Genomic Templates
-н: Raymond Vázquez, зэрэг
Хэвлэсэн: (1999-01-01)

PCRS: Personalized Career-Path Recommender System for Engineering Students
-н: Manar Qamhieh, зэрэг
Хэвлэсэн: (2020-01-01)

Using Malaise Traps and Metabarcoding for Biodiversity Assessment in Vineyards: Effects of Weather and Trapping Effort
-н: Marvin Kaczmarek, зэрэг
Хэвлэсэн: (2022-05-01)

It's raining species: Rainwash eDNA metabarcoding as a minimally invasive method to assess tree canopy invertebrate diversity
-н: Till‐Hendrik Macher, зэрэг
Хэвлэсэн: (2023-01-01)

Exploring the utility of bioaerosol metagenomics compared to PCRs for swine pathogen surveillance
-н: Adrian Muwonge, зэрэг
Хэвлэсэн: (2024-10-01)

Characterizing Crustose Lichen Communities—DNA Metabarcoding Reveals More than Meets the Eye
-н: Jacob R. Henrie, зэрэг
Хэвлэсэн: (2022-09-01)

Characterizing parasitic nematode faunas in faeces and soil using DNA metabarcoding
-н: Marie Louise Davey, зэрэг
Хэвлэсэн: (2021-08-01)

Allele-Specific Dual PCRs to Identify Members of the 27a Cluster of PPV
-н: Vivien Tamás, зэрэг
Хэвлэсэн: (2022-07-01)

High-throughput multiplex qPCRs for the surveillance of zoonotic species of canine hookworms.
-н: Luca Massetti, зэрэг
Хэвлэсэн: (2020-06-01)

Ethanol eDNA Reveals Unique Community Composition of Aquatic Macroinvertebrates Compared to Bulk Tissue Metabarcoding in a Biomonitoring Sampling Scheme
-н: Sadhna Fiona Persaud, зэрэг
Хэвлэсэн: (2021-01-01)

Long PCRs of Transposons in the Structural Analysis of Genes Encoding Acquired Glycopeptide Resistance in Enterococci
-н: H. Haaheim, зэрэг
Хэвлэсэн: (1998-03-01)

Development of two multiplex PCRs for microsatellite analysis in Alpine chamois (Rupicapra r. rupicapra)
-н: P. Sacchi, зэрэг
Хэвлэсэн: (2010-01-01)

The Phylogeny, Biodiversity, and Ecology of the Chloroflexi in Activated Sludge
-н: Lachlan B. M. Speirs, зэрэг
Хэвлэсэн: (2019-09-01)

Comparison of Pan-Lyssavirus RT-PCRs and Development of an Improved Protocol for Surveillance of Non-RABV Lyssaviruses
-н: Petra Drzewnioková, зэрэг
Хэвлэсэн: (2023-03-01)

Prevalence and detection of Tropheryma whipplei in the stools of Korean patients with diarrhea using real-time PCRs
-н: Sumi Yoon, зэрэг
Хэвлэсэн: (2022-12-01)

Outpatient palliative care referral system (PCRS) for patients with advanced cancer: an impact evaluation protocol
-н: Filippo de Braud, зэрэг
Хэвлэсэн: (2022-10-01)

Adjuvant immunotherapy in resected esophageal squamous cell carcinoma: a gospel to the non-pCRs
-н: Zhihui Zhang, зэрэг
Хэвлэсэн: (2021-08-01)

Biodiversity assessment of tropical shelf eukaryotic communities via pelagic eDNA metabarcoding
-н: Judith Bakker, зэрэг
Хэвлэсэн: (2019-12-01)

PERFORMANCE OF CONVENTIONAL PCRs BASED ON PRIMERS DIRECTED TO NUCLEAR AND MITOCHONDRIAL GENES FOR THE DETECTION AND IDENTIFICATION OF Leishmania spp.
-н: Estela Gallucci LOPES, зэрэг
Хэвлэсэн: (2016-01-01)

Novel High-Throughput Multiplex qPCRs for the Detection of Canine Vector-Borne Pathogens in the Asia-Pacific
-н: Lucas Huggins, зэрэг
Хэвлэсэн: (2021-05-01)

New Real-Time PCRs to Differentiate <i>Rickettsia</i> spp. and <i>Rickettsia conorii</i>
-н: Valeria Blanda, зэрэг
Хэвлэсэн: (2020-09-01)

Comparative Evaluation of Three Commercial Quantitative Real-Time PCRs Used in Japan for Bovine Leukemia Virus
-н: Syuji Yoneyama, зэрэг
Хэвлэсэн: (2022-05-01)

Brazilian Portuguese version of the Patient Competency Rating Scale (PCRS-R-BR): semantic adaptation and validity
-н: Nicolle Zimmermann, зэрэг
Хэвлэсэн: (2014-03-01)

Fruticose Lichen Communities at the Edge: Distribution and Diversity in a Desert Sky Island on the Colorado Plateau
-н: Abigail Robison, зэрэг
Хэвлэсэн: (2022-09-01)

mbctools: A User-Friendly Metabarcoding and Cross-Platform Pipeline for Analyzing Multiple Amplicon Sequencing Data across a Large Diversity of Organisms
-н: Barnabé, Christian, зэрэг
Хэвлэсэн: (2024-12-01)

Navigating diversity: primer impact on arthropod recovery in molecular environmental monitoring in tropical Amazon
-н: Fernanda Valente Penner, зэрэг
Хэвлэсэн: (2024-12-01)

Home is where the hollow is: Revealing vertebrate tree hollow user biodiversity with eDNA metabarcoding
-н: Joshua P. Newton, зэрэг
Хэвлэсэн: (2022-09-01)

Diatom metabarcoding as a tool to assess the water quality of two large tributaries of the Danube River
-н: Danijela Vidaković, зэрэг
Хэвлэсэн: (2024-11-01)

Optimizing insect metabarcoding using replicated mock communities
-н: Elzbieta Iwaszkiewicz‐Eggebrecht, зэрэг
Хэвлэсэн: (2023-04-01)

Fauna de Alticini (Newman) (Coleoptera, Chrysomelidae, Galerucinae) em diferentes estágios sucessionais na Floresta com Araucária do Paraná, Brasil: diversidade e estimativa de riqueza de espécies Alticini (Newman) (Coleoptera, Chrysomelidae, Galerucinae) fauna in different successional stages in an Araucaria Forest of Paraná, Brazil: diversity and estimation of species richness
-н: Adelita Maria Linzmeier, зэрэг
Хэвлэсэн: (2006-03-01)

Percevejos (Hemiptera: Heteroptera) em plantio de Eucalyptus urophylla e Floresta Estacional Semidecidual Montana no Estado da Bahia
-н: Ângela Neves Azevêdo, зэрэг
Хэвлэсэн: (2022-08-01)

Detection of non-pathogenic and pathogenic populations of Vibrio parahaemolyticus in various samples by the conventional, quantitative and droplet digital PCRs
-н: Sinisa Vidovic, зэрэг
Хэвлэсэн: (2024-02-01)

Comparative Assessment of In-House Real-Time PCRs Targeting Enteric Disease-Associated Microsporidia in Human Stool Samples
-н: Konstantin Tanida, зэрэг
Хэвлэсэн: (2021-05-01)

COMPARAÇÃO DA SENSIBILIDADE DE DIFERENTES PCRS PARA A DETECÇÃO DO DNA DE BARTONELLA HENSELAE
-н: Marina Rovani Drummond, зэрэг
Хэвлэсэн: (2023-10-01)

Multiplex real-time PCRs for detection of Salmonella, Listeria monocytogenes, and verotoxigenic Escherichia coli in carcasses of slaughtered animals
-н: Denis Edyta, зэрэг
Хэвлэсэн: (2016-09-01)

Development of O-antigen gene cluster-specific PCRs for rapid typing six epidemic serogroups of <it>Leptospira </it>in China
-н: Qin Jin-Hong, зэрэг
Хэвлэсэн: (2010-03-01)

Robber flies and hover flies (Insecta, Diptera, Asilidae and Syrphidae) in beech forests of the central Apennines: a contribution to the inventory of insect biodiversity in Italian State Nature Reserves
-н: Alice Lenzi, зэрэг
Хэвлэсэн: (2023-05-01)