Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Abstract DNA metabarcoding is a powerful tool to assess arthropod diversity in environmental bulk samples such as Malaise trap, pitfall trap, or hand net samples. While comparative performance tests for different extraction protocols, primers, and Taq polymerases have been made, the effect of differ...

Полное описание

Библиографические подробности
Главные авторы:	Dominik Buchner, Arne J. Beermann, Florian Leese, Martina Weiss
Формат:	Статья
Язык:	English
Опубликовано:	Wiley 2021-07-01
Серии:	Ecology and Evolution
Предметы:	amplicon sequencing biomonitoring bulk sample Malaise trap PCR bias
Online-ссылка:	https://doi.org/10.1002/ece3.7753

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