Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Cooking small and large portions of “biodiversity‐soup”: Miniaturized DNA metabarcoding PCRs perform as good as large‐volume PCRs

Abstract DNA metabarcoding is a powerful tool to assess arthropod diversity in environmental bulk samples such as Malaise trap, pitfall trap, or hand net samples. While comparative performance tests for different extraction protocols, primers, and Taq polymerases have been made, the effect of differ...

Szczegółowa specyfikacja

Opis bibliograficzny
Główni autorzy: Dominik Buchner, Arne J. Beermann, Florian Leese, Martina Weiss
Format: Artykuł
Język:English
Wydane: Wiley 2021-07-01
Seria:Ecology and Evolution
Hasła przedmiotowe:
amplicon sequencing
biomonitoring
bulk sample
Malaise trap
PCR bias
Dostęp online:https://doi.org/10.1002/ece3.7753

Internet

https://doi.org/10.1002/ece3.7753

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