Fluorescent Single-Core and Multi-Core Nanoprobes as Cell Trackers and Magnetic Nanoheaters
Iron oxide magnetic nanoparticles (MNPs) have been widely studied due to their versatility for diagnosis, tracking (magnetic resonance imaging (MRI)) and therapeutic (magnetic hyperthermia and drug delivery) applications. In this work, iron oxide MNPs with different single-core (8–40 nm) and multi-c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Magnetochemistry |
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| Online Access: | https://www.mdpi.com/2312-7481/8/8/83 |
| _version_ | 1797409248905265152 |
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| author | Pelayo García Acevedo Manuel A. González Gómez Ángela Arnosa Prieto Lisandra De Castro Alves Román Seco Gudiña Yolanda Piñeiro José Rivas |
| author_facet | Pelayo García Acevedo Manuel A. González Gómez Ángela Arnosa Prieto Lisandra De Castro Alves Román Seco Gudiña Yolanda Piñeiro José Rivas |
| author_sort | Pelayo García Acevedo |
| collection | DOAJ |
| description | Iron oxide magnetic nanoparticles (MNPs) have been widely studied due to their versatility for diagnosis, tracking (magnetic resonance imaging (MRI)) and therapeutic (magnetic hyperthermia and drug delivery) applications. In this work, iron oxide MNPs with different single-core (8–40 nm) and multi-core (140–200 nm) structures were synthesized and functionalized by organic and inorganic coating materials, highlighting their ability as magnetic nanotools to boost cell biotechnological procedures. Single core Fe<sub>3</sub>O<sub>4</sub>@PDA, Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>-FITC-SiO<sub>2</sub> and Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>-RITC-SiO<sub>2</sub> MNPs were functionalized with fluorescent components with emission at different wavelengths, 424 nm (polydopamine), 515 (fluorescein) and 583 nm (rhodamine), and their ability as transfection and imaging agents was explored with HeLa cells. Moreover, different multi-core iron oxide MNPs (Fe<sub>3</sub>O<sub>4</sub>@CS, Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> and Fe<sub>3</sub>O<sub>4</sub>@Citrate) coated with organic (citrate and chitosan, CS) and inorganic (silica, SiO<sub>2</sub>) shells were tested as efficient nanoheaters for magnetic hyperthermia applications for mild thermal heating procedures as an alternative to simple structures based on single-core MNPs. This work highlights the multiple abilities offered by the synergy of the use of external magnetic fields applied on MNPs and their application in different biomedical approaches. |
| first_indexed | 2024-03-09T04:10:36Z |
| format | Article |
| id | doaj.art-5654679fdd8e4fbca0ab8741bde236f5 |
| institution | Directory Open Access Journal |
| issn | 2312-7481 |
| language | English |
| last_indexed | 2024-03-09T04:10:36Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Magnetochemistry |
| spelling | doaj.art-5654679fdd8e4fbca0ab8741bde236f52023-12-03T14:00:17ZengMDPI AGMagnetochemistry2312-74812022-07-01888310.3390/magnetochemistry8080083Fluorescent Single-Core and Multi-Core Nanoprobes as Cell Trackers and Magnetic NanoheatersPelayo García Acevedo0Manuel A. González Gómez1Ángela Arnosa Prieto2Lisandra De Castro Alves3Román Seco Gudiña4Yolanda Piñeiro5José Rivas6NANOMAG Laboratory, Applied Physics Department and iMATUS Materials Institute, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, SpainNANOMAG Laboratory, Applied Physics Department and iMATUS Materials Institute, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, SpainNANOMAG Laboratory, Applied Physics Department and iMATUS Materials Institute, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, SpainNANOMAG Laboratory, Applied Physics Department and iMATUS Materials Institute, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, SpainNANOMAG Laboratory, Applied Physics Department and iMATUS Materials Institute, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, SpainNANOMAG Laboratory, Applied Physics Department and iMATUS Materials Institute, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, SpainNANOMAG Laboratory, Applied Physics Department and iMATUS Materials Institute, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, SpainIron oxide magnetic nanoparticles (MNPs) have been widely studied due to their versatility for diagnosis, tracking (magnetic resonance imaging (MRI)) and therapeutic (magnetic hyperthermia and drug delivery) applications. In this work, iron oxide MNPs with different single-core (8–40 nm) and multi-core (140–200 nm) structures were synthesized and functionalized by organic and inorganic coating materials, highlighting their ability as magnetic nanotools to boost cell biotechnological procedures. Single core Fe<sub>3</sub>O<sub>4</sub>@PDA, Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>-FITC-SiO<sub>2</sub> and Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>-RITC-SiO<sub>2</sub> MNPs were functionalized with fluorescent components with emission at different wavelengths, 424 nm (polydopamine), 515 (fluorescein) and 583 nm (rhodamine), and their ability as transfection and imaging agents was explored with HeLa cells. Moreover, different multi-core iron oxide MNPs (Fe<sub>3</sub>O<sub>4</sub>@CS, Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> and Fe<sub>3</sub>O<sub>4</sub>@Citrate) coated with organic (citrate and chitosan, CS) and inorganic (silica, SiO<sub>2</sub>) shells were tested as efficient nanoheaters for magnetic hyperthermia applications for mild thermal heating procedures as an alternative to simple structures based on single-core MNPs. This work highlights the multiple abilities offered by the synergy of the use of external magnetic fields applied on MNPs and their application in different biomedical approaches.https://www.mdpi.com/2312-7481/8/8/83iron oxide magnetic nanoparticlesnanomagnetismcell trackingmagnetic hyperthermiananowarming |
| spellingShingle | Pelayo García Acevedo Manuel A. González Gómez Ángela Arnosa Prieto Lisandra De Castro Alves Román Seco Gudiña Yolanda Piñeiro José Rivas Fluorescent Single-Core and Multi-Core Nanoprobes as Cell Trackers and Magnetic Nanoheaters Magnetochemistry iron oxide magnetic nanoparticles nanomagnetism cell tracking magnetic hyperthermia nanowarming |
| title | Fluorescent Single-Core and Multi-Core Nanoprobes as Cell Trackers and Magnetic Nanoheaters |
| title_full | Fluorescent Single-Core and Multi-Core Nanoprobes as Cell Trackers and Magnetic Nanoheaters |
| title_fullStr | Fluorescent Single-Core and Multi-Core Nanoprobes as Cell Trackers and Magnetic Nanoheaters |
| title_full_unstemmed | Fluorescent Single-Core and Multi-Core Nanoprobes as Cell Trackers and Magnetic Nanoheaters |
| title_short | Fluorescent Single-Core and Multi-Core Nanoprobes as Cell Trackers and Magnetic Nanoheaters |
| title_sort | fluorescent single core and multi core nanoprobes as cell trackers and magnetic nanoheaters |
| topic | iron oxide magnetic nanoparticles nanomagnetism cell tracking magnetic hyperthermia nanowarming |
| url | https://www.mdpi.com/2312-7481/8/8/83 |
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