Rapid Flow Cytometry Detection of a Single Viable Escherichia coli O157:H7 Cell in Raw Spinach Using a Simplified Sample Preparation Technique

Rapid Flow Cytometry Detection of a Single Viable Escherichia coli O157:H7 Cell in Raw Spinach Using a Simplified Sample Preparation Technique

Very low cell count detection of Escherichia coli O157:H7 in foods is critical, since an infective dose for this pathogen may be only 10 cells, and fewer still for vulnerable populations. A flow cytometer is able to detect and count individual cells of a target bacterium, in this case E. coli O157:H...

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Bibliographic Details
Main Authors: Anna J. Williams, Willie M. Cooper, Shawn Ramsaroop, Pierre Alusta, Dan A. Buzatu, Jon G. Wilkes
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-08-01
Series:Frontiers in Microbiology
Subjects:
sample preparation
food pathogens
bacterial detection in food
bacterial quantification
public health
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2017.01493/full

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