Dietary plasmalogen increases erythrocyte membrane plasmalogen in rats
<p>Abstract</p> <p>Background</p> <p>Many disorders with plasmalogen deficiency have been reported. Replenishment or replacement of tissue plasmalogens of these disorders would be beneficial to the patients with these disorders, but effects of dietary plasmalogen on mam...
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| Format: | Article |
| Language: | English |
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BMC
2012-11-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://www.lipidworld.com/content/11/1/161 |
| _version_ | 1818473862688407552 |
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| author | Mawatari Shiro Katafuchi Toshihiko Miake Kiyotaka Fujino Takehiko |
| author_facet | Mawatari Shiro Katafuchi Toshihiko Miake Kiyotaka Fujino Takehiko |
| author_sort | Mawatari Shiro |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Many disorders with plasmalogen deficiency have been reported. Replenishment or replacement of tissue plasmalogens of these disorders would be beneficial to the patients with these disorders, but effects of dietary plasmalogen on mammals have not been reported.</p> <p>Methods</p> <p>Plasmalogens were purified from chicken skin. The purified plasmalogens consisted of 96.4% ethanolamine plasmalogen (PlsEtn), 2.4% choline plasmalogen (PlsCho) and 0.5% sphingomyelin (SM). A diet containing 0.1% the purified plasmalogens (PlsEtn diet) was given to rats. Relative composition of phospholipids was measured by a high performance liquid chromatography (HPLC) method that can separate intact plasmalogens and all other phospholipid classes by a single chromatographic run.</p> <p>Results</p> <p>The PlsEtn diet given to Zucker diabetic fatty (ZDF) rats for 4 weeks caused decreases of plasma cholesterol and plasma phospholipid as compared to control diet. The other routine laboratory tests of plasma including triacylglycerol, glucose, liver and renal functions, albumin, and body weight were not different. Relative compositions of erythrocyte PlsEtn and phosphatidylethanolamine (PE) increased, and that of phosphatidylcholine (PC) decreased in PlsEtn diet group. The PlsEtn diet given to normal rats for 9 weeks again caused decrease of plasma cholesterol and phospholipid, and it induced increase of relative composition of PlsEtn of the erythrocyte membrane. The other routine laboratory tests of plasma and body weight were not different.</p> <p>Conclusions</p> <p>Dietary PlsEtn increases relative composition of PlsEtn of erythrocyte membranes in normal and ZDF rats, and it causes decreases of plasma cholesterol and plasma phospholipids. Dietary PlsEtn for 9 weeks seemingly causes no adverse effect to health of normal rats.</p> |
| first_indexed | 2024-04-14T04:28:23Z |
| format | Article |
| id | doaj.art-5655933534e34885a5576f9a8d091186 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-04-14T04:28:23Z |
| publishDate | 2012-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-5655933534e34885a5576f9a8d0911862022-12-22T02:12:10ZengBMCLipids in Health and Disease1476-511X2012-11-0111116110.1186/1476-511X-11-161Dietary plasmalogen increases erythrocyte membrane plasmalogen in ratsMawatari ShiroKatafuchi ToshihikoMiake KiyotakaFujino Takehiko<p>Abstract</p> <p>Background</p> <p>Many disorders with plasmalogen deficiency have been reported. Replenishment or replacement of tissue plasmalogens of these disorders would be beneficial to the patients with these disorders, but effects of dietary plasmalogen on mammals have not been reported.</p> <p>Methods</p> <p>Plasmalogens were purified from chicken skin. The purified plasmalogens consisted of 96.4% ethanolamine plasmalogen (PlsEtn), 2.4% choline plasmalogen (PlsCho) and 0.5% sphingomyelin (SM). A diet containing 0.1% the purified plasmalogens (PlsEtn diet) was given to rats. Relative composition of phospholipids was measured by a high performance liquid chromatography (HPLC) method that can separate intact plasmalogens and all other phospholipid classes by a single chromatographic run.</p> <p>Results</p> <p>The PlsEtn diet given to Zucker diabetic fatty (ZDF) rats for 4 weeks caused decreases of plasma cholesterol and plasma phospholipid as compared to control diet. The other routine laboratory tests of plasma including triacylglycerol, glucose, liver and renal functions, albumin, and body weight were not different. Relative compositions of erythrocyte PlsEtn and phosphatidylethanolamine (PE) increased, and that of phosphatidylcholine (PC) decreased in PlsEtn diet group. The PlsEtn diet given to normal rats for 9 weeks again caused decrease of plasma cholesterol and phospholipid, and it induced increase of relative composition of PlsEtn of the erythrocyte membrane. The other routine laboratory tests of plasma and body weight were not different.</p> <p>Conclusions</p> <p>Dietary PlsEtn increases relative composition of PlsEtn of erythrocyte membranes in normal and ZDF rats, and it causes decreases of plasma cholesterol and plasma phospholipids. Dietary PlsEtn for 9 weeks seemingly causes no adverse effect to health of normal rats.</p>http://www.lipidworld.com/content/11/1/161Dietary plasmalogenZucker diabetic fatty ratWistar ratErythrocyte phospholipidsPlasma phospholipids |
| spellingShingle | Mawatari Shiro Katafuchi Toshihiko Miake Kiyotaka Fujino Takehiko Dietary plasmalogen increases erythrocyte membrane plasmalogen in rats Lipids in Health and Disease Dietary plasmalogen Zucker diabetic fatty rat Wistar rat Erythrocyte phospholipids Plasma phospholipids |
| title | Dietary plasmalogen increases erythrocyte membrane plasmalogen in rats |
| title_full | Dietary plasmalogen increases erythrocyte membrane plasmalogen in rats |
| title_fullStr | Dietary plasmalogen increases erythrocyte membrane plasmalogen in rats |
| title_full_unstemmed | Dietary plasmalogen increases erythrocyte membrane plasmalogen in rats |
| title_short | Dietary plasmalogen increases erythrocyte membrane plasmalogen in rats |
| title_sort | dietary plasmalogen increases erythrocyte membrane plasmalogen in rats |
| topic | Dietary plasmalogen Zucker diabetic fatty rat Wistar rat Erythrocyte phospholipids Plasma phospholipids |
| url | http://www.lipidworld.com/content/11/1/161 |
| work_keys_str_mv | AT mawatarishiro dietaryplasmalogenincreaseserythrocytemembraneplasmalogeninrats AT katafuchitoshihiko dietaryplasmalogenincreaseserythrocytemembraneplasmalogeninrats AT miakekiyotaka dietaryplasmalogenincreaseserythrocytemembraneplasmalogeninrats AT fujinotakehiko dietaryplasmalogenincreaseserythrocytemembraneplasmalogeninrats |