Exploratory Study on the Foliar Incorporation and Stability of Isotopically Labeled Amino Acids Applied to Turfgrass
There is increasing interest in the use of amino acid-based biostimulant products due to their reported abilities to improve a number of quality characteristics in a variety of specialty crops. However, when it comes to the foliar application of amino acids to turfgrass, there are still many basic q...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/3/358 |
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| author | Rachel M. McCoy George W. Meyer David Rhodes George C. Murray Thomas G. Sors Joshua R. Widhalm |
| author_facet | Rachel M. McCoy George W. Meyer David Rhodes George C. Murray Thomas G. Sors Joshua R. Widhalm |
| author_sort | Rachel M. McCoy |
| collection | DOAJ |
| description | There is increasing interest in the use of amino acid-based biostimulant products due to their reported abilities to improve a number of quality characteristics in a variety of specialty crops. However, when it comes to the foliar application of amino acids to turfgrass, there are still many basic questions about their uptake forms and incorporation into cellular metabolism. In this study, we shed light on the fate of amino acids exogenously applied to turfgrass foliage through a series of time-course, isotopic-labeling studies in creeping bentgrass (<i>Agrostis stolonifera</i> L.) leaves. Using both <sup>15</sup>N-labeled and <sup>15</sup>N,<sup>13</sup>C double-labeled L-glutamate applied exogenously to creeping bentgrass foliage, we measured the uptake of glutamate and its integration into γ-aminobutyric acid (GABA) and L-proline, two amino acids with known roles in plant stress adaptation. Our results demonstrate that glutamate is rapidly absorbed into creeping bentgrass foliage and that it is utilized to produce GABA and proline. Based on the labeling patterns observed in the endogenous pools of glutamate/glutamine, GABA, and the proline from applied glutamate-[<sup>13</sup>C<sub>5</sub><sup>15</sup>N<sub>1</sub>], we can further conclude that glutamate is predominantly taken up intact and that mineralization into other forms of nitrogen is a minor fate. Taken together, the collective findings of this study provide evidence that amino acids exogenously applied to turfgrass foliage can be rapidly absorbed, and serve as stable sources of precursor molecules to be integrated into the metabolism of the plant. |
| first_indexed | 2024-12-14T10:35:48Z |
| format | Article |
| id | doaj.art-5656a344d90942e5b28bbce894d1fcb2 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-12-14T10:35:48Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-5656a344d90942e5b28bbce894d1fcb22022-12-21T23:05:57ZengMDPI AGAgronomy2073-43952020-03-0110335810.3390/agronomy10030358agronomy10030358Exploratory Study on the Foliar Incorporation and Stability of Isotopically Labeled Amino Acids Applied to TurfgrassRachel M. McCoy0George W. Meyer1David Rhodes2George C. Murray3Thomas G. Sors4Joshua R. Widhalm5Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USADepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USADepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USAEnP Investments, LLC, 2001 West Main Street, Mendota, IL 61342, USAPurdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, West Lafayette, IN 47907, USADepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USAThere is increasing interest in the use of amino acid-based biostimulant products due to their reported abilities to improve a number of quality characteristics in a variety of specialty crops. However, when it comes to the foliar application of amino acids to turfgrass, there are still many basic questions about their uptake forms and incorporation into cellular metabolism. In this study, we shed light on the fate of amino acids exogenously applied to turfgrass foliage through a series of time-course, isotopic-labeling studies in creeping bentgrass (<i>Agrostis stolonifera</i> L.) leaves. Using both <sup>15</sup>N-labeled and <sup>15</sup>N,<sup>13</sup>C double-labeled L-glutamate applied exogenously to creeping bentgrass foliage, we measured the uptake of glutamate and its integration into γ-aminobutyric acid (GABA) and L-proline, two amino acids with known roles in plant stress adaptation. Our results demonstrate that glutamate is rapidly absorbed into creeping bentgrass foliage and that it is utilized to produce GABA and proline. Based on the labeling patterns observed in the endogenous pools of glutamate/glutamine, GABA, and the proline from applied glutamate-[<sup>13</sup>C<sub>5</sub><sup>15</sup>N<sub>1</sub>], we can further conclude that glutamate is predominantly taken up intact and that mineralization into other forms of nitrogen is a minor fate. Taken together, the collective findings of this study provide evidence that amino acids exogenously applied to turfgrass foliage can be rapidly absorbed, and serve as stable sources of precursor molecules to be integrated into the metabolism of the plant.https://www.mdpi.com/2073-4395/10/3/358biostimulantsamino acidsisotopic labelingturfgrass |
| spellingShingle | Rachel M. McCoy George W. Meyer David Rhodes George C. Murray Thomas G. Sors Joshua R. Widhalm Exploratory Study on the Foliar Incorporation and Stability of Isotopically Labeled Amino Acids Applied to Turfgrass Agronomy biostimulants amino acids isotopic labeling turfgrass |
| title | Exploratory Study on the Foliar Incorporation and Stability of Isotopically Labeled Amino Acids Applied to Turfgrass |
| title_full | Exploratory Study on the Foliar Incorporation and Stability of Isotopically Labeled Amino Acids Applied to Turfgrass |
| title_fullStr | Exploratory Study on the Foliar Incorporation and Stability of Isotopically Labeled Amino Acids Applied to Turfgrass |
| title_full_unstemmed | Exploratory Study on the Foliar Incorporation and Stability of Isotopically Labeled Amino Acids Applied to Turfgrass |
| title_short | Exploratory Study on the Foliar Incorporation and Stability of Isotopically Labeled Amino Acids Applied to Turfgrass |
| title_sort | exploratory study on the foliar incorporation and stability of isotopically labeled amino acids applied to turfgrass |
| topic | biostimulants amino acids isotopic labeling turfgrass |
| url | https://www.mdpi.com/2073-4395/10/3/358 |
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