Evaluation of biocatalysts synthesized with lipase from Pseudomonas cepacia supported on glycol-modified MOF-199 in the synthesis of green biodiesel
In this work, we report the synthesis and characterization of biocatalysts prepared using MOF-199 and lipase from Pseudomonas cepacia as a support, an agent for the breakdown of saturated, monounsaturated, and polyunsaturated fatty acids, the main components of palm oil (Elaeis guineensis), used to...
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Sustainability |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/frsus.2022.941131/full |
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| author | José Manuel Martínez Gil José Manuel Martínez Gil José Manuel Martínez Gil José Manuel Martínez Gil Ricardo Vivas-Reyes Marlón José Bastidas-Barranco Liliana Giraldo Juan Carlos Moreno-Piraján |
| author_facet | José Manuel Martínez Gil José Manuel Martínez Gil José Manuel Martínez Gil José Manuel Martínez Gil Ricardo Vivas-Reyes Marlón José Bastidas-Barranco Liliana Giraldo Juan Carlos Moreno-Piraján |
| author_sort | José Manuel Martínez Gil |
| collection | DOAJ |
| description | In this work, we report the synthesis and characterization of biocatalysts prepared using MOF-199 and lipase from Pseudomonas cepacia as a support, an agent for the breakdown of saturated, monounsaturated, and polyunsaturated fatty acids, the main components of palm oil (Elaeis guineensis), used to produce green biodiesel. Lipase from Pseudomonas cepacia (PCL) is used as an enzyme, which is supported by adsorption on MOF-199 (MOF-199-PCL) and another part of MOF-199 is modified with glycol as a carbon source to which PCL is supported to obtain the modified MOF (Gly@MOF-199-PCL). MOF-199 was modified by calcining a sample at 900°C for 2 h at a heating rate of 7°C/min, in an inert atmosphere and then stored in a desiccator to protect it from moisture before proceeding to adsorb PCL. Both the biocatalysts (MOF-199-PCL and Gly@MOF-199-PCL) and the supernatant liquid were characterized using Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, energy dispersive X-ray spectroscopy (EDS or EDX), gas chromatography coupled with mass (GC-MS), and nuclear magnetic resonance (NMR). The results of the instrumental part show that both biocatalysts produce green biodiesel, which is a novel contribution to these systems. |
| first_indexed | 2024-04-13T21:27:43Z |
| format | Article |
| id | doaj.art-56571e35b65344a7b81dae276527ac1d |
| institution | Directory Open Access Journal |
| issn | 2673-4524 |
| language | English |
| last_indexed | 2024-04-13T21:27:43Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sustainability |
| spelling | doaj.art-56571e35b65344a7b81dae276527ac1d2022-12-22T02:29:16ZengFrontiers Media S.A.Frontiers in Sustainability2673-45242022-11-01310.3389/frsus.2022.941131941131Evaluation of biocatalysts synthesized with lipase from Pseudomonas cepacia supported on glycol-modified MOF-199 in the synthesis of green biodieselJosé Manuel Martínez Gil0José Manuel Martínez Gil1José Manuel Martínez Gil2José Manuel Martínez Gil3Ricardo Vivas-Reyes4Marlón José Bastidas-Barranco5Liliana Giraldo6Juan Carlos Moreno-Piraján7Grupo de Investigación Catálisis y Materiales, Facultad de Ciencias Básicas y Aplicadas, Universidad de La Guajira, Riohacha, ColombiaGrupo de Investigación Química Cuántica y Teórica, Facultad de Ciencias Exactas y Naturales, Universidad de Cartagena, Cartagena, ColombiaGrupo de Investigación Desarrollo de Estudios y Tecnologías Ambientales del Carbono (DESTACAR), Facultad de Ingenierías, Universidad de La Guajira, Riohacha, ColombiaGrupo de Investigación en Sólidos Porosos y Calorimetría, Facultad de Ciencias, Departamento de Química, Universidad de los Andes, Bogotá, ColombiaGrupo de Investigación Química Cuántica y Teórica, Facultad de Ciencias Exactas y Naturales, Universidad de Cartagena, Cartagena, ColombiaGrupo de Investigación Desarrollo de Estudios y Tecnologías Ambientales del Carbono (DESTACAR), Facultad de Ingenierías, Universidad de La Guajira, Riohacha, ColombiaGrupo de Calorimetría, Facultad de Ciencias, Departamento de Química, Universidad Nacional de Colombia, Bogotá, ColombiaGrupo de Investigación en Sólidos Porosos y Calorimetría, Facultad de Ciencias, Departamento de Química, Universidad de los Andes, Bogotá, ColombiaIn this work, we report the synthesis and characterization of biocatalysts prepared using MOF-199 and lipase from Pseudomonas cepacia as a support, an agent for the breakdown of saturated, monounsaturated, and polyunsaturated fatty acids, the main components of palm oil (Elaeis guineensis), used to produce green biodiesel. Lipase from Pseudomonas cepacia (PCL) is used as an enzyme, which is supported by adsorption on MOF-199 (MOF-199-PCL) and another part of MOF-199 is modified with glycol as a carbon source to which PCL is supported to obtain the modified MOF (Gly@MOF-199-PCL). MOF-199 was modified by calcining a sample at 900°C for 2 h at a heating rate of 7°C/min, in an inert atmosphere and then stored in a desiccator to protect it from moisture before proceeding to adsorb PCL. Both the biocatalysts (MOF-199-PCL and Gly@MOF-199-PCL) and the supernatant liquid were characterized using Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, energy dispersive X-ray spectroscopy (EDS or EDX), gas chromatography coupled with mass (GC-MS), and nuclear magnetic resonance (NMR). The results of the instrumental part show that both biocatalysts produce green biodiesel, which is a novel contribution to these systems.https://www.frontiersin.org/articles/10.3389/frsus.2022.941131/fullmetal-organic frameworkadsorptiongreen biodiesellipasesbiocatalysts |
| spellingShingle | José Manuel Martínez Gil José Manuel Martínez Gil José Manuel Martínez Gil José Manuel Martínez Gil Ricardo Vivas-Reyes Marlón José Bastidas-Barranco Liliana Giraldo Juan Carlos Moreno-Piraján Evaluation of biocatalysts synthesized with lipase from Pseudomonas cepacia supported on glycol-modified MOF-199 in the synthesis of green biodiesel Frontiers in Sustainability metal-organic framework adsorption green biodiesel lipases biocatalysts |
| title | Evaluation of biocatalysts synthesized with lipase from Pseudomonas cepacia supported on glycol-modified MOF-199 in the synthesis of green biodiesel |
| title_full | Evaluation of biocatalysts synthesized with lipase from Pseudomonas cepacia supported on glycol-modified MOF-199 in the synthesis of green biodiesel |
| title_fullStr | Evaluation of biocatalysts synthesized with lipase from Pseudomonas cepacia supported on glycol-modified MOF-199 in the synthesis of green biodiesel |
| title_full_unstemmed | Evaluation of biocatalysts synthesized with lipase from Pseudomonas cepacia supported on glycol-modified MOF-199 in the synthesis of green biodiesel |
| title_short | Evaluation of biocatalysts synthesized with lipase from Pseudomonas cepacia supported on glycol-modified MOF-199 in the synthesis of green biodiesel |
| title_sort | evaluation of biocatalysts synthesized with lipase from pseudomonas cepacia supported on glycol modified mof 199 in the synthesis of green biodiesel |
| topic | metal-organic framework adsorption green biodiesel lipases biocatalysts |
| url | https://www.frontiersin.org/articles/10.3389/frsus.2022.941131/full |
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