A <i>Medicago truncatula</i> Metabolite Atlas Enables the Visualization of Differential Accumulation of Metabolites in Root Tissues
Plant roots are composed of many differentiated tissue types, with each tissue exhibiting differential quantitative and qualitative accumulation of metabolites. The large-scale nontargeted metabolite profiles of these differentiated tissues are complex, which complicates the interpretation and devel...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/4/238 |
| _version_ | 1827695196434857984 |
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| author | Clayton Kranawetter Shuai Zeng Trupti Joshi Lloyd W. Sumner |
| author_facet | Clayton Kranawetter Shuai Zeng Trupti Joshi Lloyd W. Sumner |
| author_sort | Clayton Kranawetter |
| collection | DOAJ |
| description | Plant roots are composed of many differentiated tissue types, with each tissue exhibiting differential quantitative and qualitative accumulation of metabolites. The large-scale nontargeted metabolite profiles of these differentiated tissues are complex, which complicates the interpretation and development of hypotheses relative to the biological roles of differentially localized metabolites. Thus, we created a data visualization tool to aid in the visualization and understanding of differential metabolite accumulations in <i>Medicago truncatula</i> roots. This was achieved through the development of the <i>Medicago truncatula</i> Metabolite Atlas based upon an adaptation of the Arabidopsis Electronic Fluorescent Pictograph (eFP) Browser. <i>Medicago truncatula</i> roots were dissected into border cells, root cap, elongation zone, mature root, and root secretions. Each tissue was then analyzed by UHPLC-QTOF-MS and GC-Q-MS. Data were uploaded into a MySQL database and displayed in the <i>Medicago truncatula</i> Metabolite Atlas. The data revealed unique differential spatial localization of many metabolites, some of which are discussed here. Ultimately, the <i>Medicago truncatula</i> Metabolite Atlas compiles metabolite data into a singular, useful, and publicly available web-based tool that enables the visualization and understanding of differential metabolite accumulation and spatial localization. |
| first_indexed | 2024-03-10T12:22:05Z |
| format | Article |
| id | doaj.art-565961273b554e57989722ef149b780c |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T12:22:05Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-565961273b554e57989722ef149b780c2023-11-21T15:24:51ZengMDPI AGMetabolites2218-19892021-04-0111423810.3390/metabo11040238A <i>Medicago truncatula</i> Metabolite Atlas Enables the Visualization of Differential Accumulation of Metabolites in Root TissuesClayton Kranawetter0Shuai Zeng1Trupti Joshi2Lloyd W. Sumner3Department of Biochemistry, University of Missouri-Columbia, Columbia, MO 65211, USAChristopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USAChristopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USADepartment of Biochemistry, University of Missouri-Columbia, Columbia, MO 65211, USAPlant roots are composed of many differentiated tissue types, with each tissue exhibiting differential quantitative and qualitative accumulation of metabolites. The large-scale nontargeted metabolite profiles of these differentiated tissues are complex, which complicates the interpretation and development of hypotheses relative to the biological roles of differentially localized metabolites. Thus, we created a data visualization tool to aid in the visualization and understanding of differential metabolite accumulations in <i>Medicago truncatula</i> roots. This was achieved through the development of the <i>Medicago truncatula</i> Metabolite Atlas based upon an adaptation of the Arabidopsis Electronic Fluorescent Pictograph (eFP) Browser. <i>Medicago truncatula</i> roots were dissected into border cells, root cap, elongation zone, mature root, and root secretions. Each tissue was then analyzed by UHPLC-QTOF-MS and GC-Q-MS. Data were uploaded into a MySQL database and displayed in the <i>Medicago truncatula</i> Metabolite Atlas. The data revealed unique differential spatial localization of many metabolites, some of which are discussed here. Ultimately, the <i>Medicago truncatula</i> Metabolite Atlas compiles metabolite data into a singular, useful, and publicly available web-based tool that enables the visualization and understanding of differential metabolite accumulation and spatial localization.https://www.mdpi.com/2218-1989/11/4/238metabolomicsrootsrhizosphere<i>Medicago truncatula</i>border cellsMetabolite Atlas |
| spellingShingle | Clayton Kranawetter Shuai Zeng Trupti Joshi Lloyd W. Sumner A <i>Medicago truncatula</i> Metabolite Atlas Enables the Visualization of Differential Accumulation of Metabolites in Root Tissues Metabolites metabolomics roots rhizosphere <i>Medicago truncatula</i> border cells Metabolite Atlas |
| title | A <i>Medicago truncatula</i> Metabolite Atlas Enables the Visualization of Differential Accumulation of Metabolites in Root Tissues |
| title_full | A <i>Medicago truncatula</i> Metabolite Atlas Enables the Visualization of Differential Accumulation of Metabolites in Root Tissues |
| title_fullStr | A <i>Medicago truncatula</i> Metabolite Atlas Enables the Visualization of Differential Accumulation of Metabolites in Root Tissues |
| title_full_unstemmed | A <i>Medicago truncatula</i> Metabolite Atlas Enables the Visualization of Differential Accumulation of Metabolites in Root Tissues |
| title_short | A <i>Medicago truncatula</i> Metabolite Atlas Enables the Visualization of Differential Accumulation of Metabolites in Root Tissues |
| title_sort | i medicago truncatula i metabolite atlas enables the visualization of differential accumulation of metabolites in root tissues |
| topic | metabolomics roots rhizosphere <i>Medicago truncatula</i> border cells Metabolite Atlas |
| url | https://www.mdpi.com/2218-1989/11/4/238 |
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