Immunomodulatory effect of structurally-characterized mushroom sclerotial polysaccharides isolated from Polyporus rhinocerus on human monoctyes THP-1
Two high molecular weight (MW larger than 788 kDa) mushroom polysaccharides extracted by hot water (PRW) and micronized sonication-assisted cold alkali (mPRSon), were isolated from the sclerotia of Polyporus rhinocerus. PRW was a polysaccharide-protein complex with a highly branched (degree of branc...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-02-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464617307636 |
| _version_ | 1819044362351280128 |
|---|---|
| author | Chaoran Liu Man Wing Choi Xiaojie Li Peter C.K. Cheung |
| author_facet | Chaoran Liu Man Wing Choi Xiaojie Li Peter C.K. Cheung |
| author_sort | Chaoran Liu |
| collection | DOAJ |
| description | Two high molecular weight (MW larger than 788 kDa) mushroom polysaccharides extracted by hot water (PRW) and micronized sonication-assisted cold alkali (mPRSon), were isolated from the sclerotia of Polyporus rhinocerus. PRW was a polysaccharide-protein complex with a highly branched (degree of branching DB 0.60) β-D-mannoglucan while mPRSon was a hyper-branched β-glucan (DB 0.85). PRW could bind to complement receptor 3 (CR3) and significantly induced THP-1 differentiation evidenced by cell morphology and enhanced phagocytic activity. A significant increase in the expression of surface markers including CD14, CD11b and HLA-DR as well as enhanced production of cytokines including IL-8, RANTES, GRO, MCP-1, MCP-2 and IL-6 were observed on THP-1 cells treated with PRW. In contrast, mPRSon could not either activate the Dectin-1 signaling or induce the THP-1 differentiation. High MW and DB mushroom sclerotial polysaccharide which has a structure of heteroglycan and protein is more immunopotent than pure homoglycan. |
| first_indexed | 2024-12-21T10:11:28Z |
| format | Article |
| id | doaj.art-565d8616216a4bca84212c4ca2326d6e |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-21T10:11:28Z |
| publishDate | 2018-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-565d8616216a4bca84212c4ca2326d6e2022-12-21T19:07:42ZengElsevierJournal of Functional Foods1756-46462018-02-01419099Immunomodulatory effect of structurally-characterized mushroom sclerotial polysaccharides isolated from Polyporus rhinocerus on human monoctyes THP-1Chaoran Liu0Man Wing Choi1Xiaojie Li2Peter C.K. Cheung3Food and Nutritional Sciences, School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region; Food and Drug Safety Research Centre, Shenzhen Institute of Standards and Technology, Shenzhen, ChinaFood and Nutritional Sciences, School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative RegionFood and Nutritional Sciences, School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative RegionFood and Nutritional Sciences, School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region; Corresponding author.Two high molecular weight (MW larger than 788 kDa) mushroom polysaccharides extracted by hot water (PRW) and micronized sonication-assisted cold alkali (mPRSon), were isolated from the sclerotia of Polyporus rhinocerus. PRW was a polysaccharide-protein complex with a highly branched (degree of branching DB 0.60) β-D-mannoglucan while mPRSon was a hyper-branched β-glucan (DB 0.85). PRW could bind to complement receptor 3 (CR3) and significantly induced THP-1 differentiation evidenced by cell morphology and enhanced phagocytic activity. A significant increase in the expression of surface markers including CD14, CD11b and HLA-DR as well as enhanced production of cytokines including IL-8, RANTES, GRO, MCP-1, MCP-2 and IL-6 were observed on THP-1 cells treated with PRW. In contrast, mPRSon could not either activate the Dectin-1 signaling or induce the THP-1 differentiation. High MW and DB mushroom sclerotial polysaccharide which has a structure of heteroglycan and protein is more immunopotent than pure homoglycan.http://www.sciencedirect.com/science/article/pii/S1756464617307636Human monocytesImmunomodulationMushroom sclerotiaPolyporus rhinocerusPolysaccharidesTHP-1 |
| spellingShingle | Chaoran Liu Man Wing Choi Xiaojie Li Peter C.K. Cheung Immunomodulatory effect of structurally-characterized mushroom sclerotial polysaccharides isolated from Polyporus rhinocerus on human monoctyes THP-1 Journal of Functional Foods Human monocytes Immunomodulation Mushroom sclerotia Polyporus rhinocerus Polysaccharides THP-1 |
| title | Immunomodulatory effect of structurally-characterized mushroom sclerotial polysaccharides isolated from Polyporus rhinocerus on human monoctyes THP-1 |
| title_full | Immunomodulatory effect of structurally-characterized mushroom sclerotial polysaccharides isolated from Polyporus rhinocerus on human monoctyes THP-1 |
| title_fullStr | Immunomodulatory effect of structurally-characterized mushroom sclerotial polysaccharides isolated from Polyporus rhinocerus on human monoctyes THP-1 |
| title_full_unstemmed | Immunomodulatory effect of structurally-characterized mushroom sclerotial polysaccharides isolated from Polyporus rhinocerus on human monoctyes THP-1 |
| title_short | Immunomodulatory effect of structurally-characterized mushroom sclerotial polysaccharides isolated from Polyporus rhinocerus on human monoctyes THP-1 |
| title_sort | immunomodulatory effect of structurally characterized mushroom sclerotial polysaccharides isolated from polyporus rhinocerus on human monoctyes thp 1 |
| topic | Human monocytes Immunomodulation Mushroom sclerotia Polyporus rhinocerus Polysaccharides THP-1 |
| url | http://www.sciencedirect.com/science/article/pii/S1756464617307636 |
| work_keys_str_mv | AT chaoranliu immunomodulatoryeffectofstructurallycharacterizedmushroomsclerotialpolysaccharidesisolatedfrompolyporusrhinocerusonhumanmonoctyesthp1 AT manwingchoi immunomodulatoryeffectofstructurallycharacterizedmushroomsclerotialpolysaccharidesisolatedfrompolyporusrhinocerusonhumanmonoctyesthp1 AT xiaojieli immunomodulatoryeffectofstructurallycharacterizedmushroomsclerotialpolysaccharidesisolatedfrompolyporusrhinocerusonhumanmonoctyesthp1 AT peterckcheung immunomodulatoryeffectofstructurallycharacterizedmushroomsclerotialpolysaccharidesisolatedfrompolyporusrhinocerusonhumanmonoctyesthp1 |