Comparison of Microbial Communities Associated with Halophyte (Salsola stocksii) and Non-Halophyte (Triticum aestivum) Using Culture-Independent Approache
Halophyte microbiome contributes significantly to plant performance and can provide information regarding complex ecological processes involved in osmoregulation of these plants. The objective of this study is to investigate the microbiomes associated with belowground (rhizo­sphere), interna...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2017-09-01
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| Series: | Polish Journal of Microbiology |
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| Online Access: | https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/66/3/pdf/10.5604_01.3001.0010.4866.pdf |
| _version_ | 1818173175868948480 |
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| author | Salma Mukhtar Ayesha Ishaq Sara Hassan Samina Mehnaz Muhammad S. Mirza Kauser A. Malik |
| author_facet | Salma Mukhtar Ayesha Ishaq Sara Hassan Samina Mehnaz Muhammad S. Mirza Kauser A. Malik |
| author_sort | Salma Mukhtar |
| collection | DOAJ |
| description | Halophyte microbiome contributes significantly to plant performance and can provide information regarding complex ecological processes involved in osmoregulation of these plants. The objective of this study is to investigate the microbiomes associated with belowground (rhizo­sphere), internal (endosphere) and aboveground (phyllosphere) tissues of halophyte (Salsola stocksii) through metagenomics approach. Plant samples were collected from Khewra Salt Mines. The metagenomic DNA from soil, root and shoot samples was isolated with the help of FastDNA spin kit. Through PCR, the 16S rRNA gene from four different Salsola plants and wheat plants was amplified and cloned in InsTAclone PCR cloning kit. Metagenomic analyses from rhizosphere, endosphere and phyllosphere of Salsola showed that approximately 29% bacteria were uncultured and unclassified. Proteobacteria and Actinobacteria were the most abundant phyla in Salsola and wheat. How­ever, Firmicutes, Acidobacteria, Bacteriodetes, Planctomycetes, Cyanobacteria, Thermotogae, Verrucomicrobia, Choroflexi and Euryarchaeota were predominant groups from halophyte whereas Actinobacteria, Proteobacteria, Firmicutes, Cyanobacteria, Acidobacteria, Bacteriodetes, Planctomycetes and Verrucomicrobia were predominant phyla of wheat samples. Diversity and differences of microbial flora of Salsola and wheat suggested that functional interactions between plants and microorganisms contribute to salt stress tolerance. |
| first_indexed | 2024-12-11T19:24:20Z |
| format | Article |
| id | doaj.art-565e3e38e3ba40e3a64dd25f143e8916 |
| institution | Directory Open Access Journal |
| issn | 1733-1331 2544-4646 |
| language | English |
| last_indexed | 2024-12-11T19:24:20Z |
| publishDate | 2017-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj.art-565e3e38e3ba40e3a64dd25f143e89162022-12-22T00:53:27ZengSciendoPolish Journal of Microbiology1733-13312544-46462017-09-0166310.5604/01.3001.0010.4866Comparison of Microbial Communities Associated with Halophyte (Salsola stocksii) and Non-Halophyte (Triticum aestivum) Using Culture-Independent ApproacheSalma Mukhtar0Ayesha Ishaq1Sara Hassan2Samina Mehnaz3Muhammad S. Mirza4Kauser A. Malik5Department of Biological Sciences, Forman Christian College (A Chartered University), Lahore, PakistanDepartment of Biological Sciences, Forman Christian College (A Chartered University), Lahore, PakistanDepartment of Biological Sciences, Forman Christian College (A Chartered University), Lahore, PakistanDepartment of Biological Sciences, Forman Christian College (A Chartered University), Lahore, PakistanEnvironmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, PakistanDepartment of Biological Sciences, Forman Christian College (A Chartered University), Lahore, PakistanHalophyte microbiome contributes significantly to plant performance and can provide information regarding complex ecological processes involved in osmoregulation of these plants. The objective of this study is to investigate the microbiomes associated with belowground (rhizo­sphere), internal (endosphere) and aboveground (phyllosphere) tissues of halophyte (Salsola stocksii) through metagenomics approach. Plant samples were collected from Khewra Salt Mines. The metagenomic DNA from soil, root and shoot samples was isolated with the help of FastDNA spin kit. Through PCR, the 16S rRNA gene from four different Salsola plants and wheat plants was amplified and cloned in InsTAclone PCR cloning kit. Metagenomic analyses from rhizosphere, endosphere and phyllosphere of Salsola showed that approximately 29% bacteria were uncultured and unclassified. Proteobacteria and Actinobacteria were the most abundant phyla in Salsola and wheat. How­ever, Firmicutes, Acidobacteria, Bacteriodetes, Planctomycetes, Cyanobacteria, Thermotogae, Verrucomicrobia, Choroflexi and Euryarchaeota were predominant groups from halophyte whereas Actinobacteria, Proteobacteria, Firmicutes, Cyanobacteria, Acidobacteria, Bacteriodetes, Planctomycetes and Verrucomicrobia were predominant phyla of wheat samples. Diversity and differences of microbial flora of Salsola and wheat suggested that functional interactions between plants and microorganisms contribute to salt stress tolerance.https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/66/3/pdf/10.5604_01.3001.0010.4866.pdf16S rRNA gene approachmicrobial communities associated with plantsmicrobiome of halophyte |
| spellingShingle | Salma Mukhtar Ayesha Ishaq Sara Hassan Samina Mehnaz Muhammad S. Mirza Kauser A. Malik Comparison of Microbial Communities Associated with Halophyte (Salsola stocksii) and Non-Halophyte (Triticum aestivum) Using Culture-Independent Approache Polish Journal of Microbiology 16S rRNA gene approach microbial communities associated with plants microbiome of halophyte |
| title | Comparison of Microbial Communities Associated with Halophyte (Salsola stocksii) and Non-Halophyte (Triticum aestivum) Using Culture-Independent Approache |
| title_full | Comparison of Microbial Communities Associated with Halophyte (Salsola stocksii) and Non-Halophyte (Triticum aestivum) Using Culture-Independent Approache |
| title_fullStr | Comparison of Microbial Communities Associated with Halophyte (Salsola stocksii) and Non-Halophyte (Triticum aestivum) Using Culture-Independent Approache |
| title_full_unstemmed | Comparison of Microbial Communities Associated with Halophyte (Salsola stocksii) and Non-Halophyte (Triticum aestivum) Using Culture-Independent Approache |
| title_short | Comparison of Microbial Communities Associated with Halophyte (Salsola stocksii) and Non-Halophyte (Triticum aestivum) Using Culture-Independent Approache |
| title_sort | comparison of microbial communities associated with halophyte salsola stocksii and non halophyte triticum aestivum using culture independent approache |
| topic | 16S rRNA gene approach microbial communities associated with plants microbiome of halophyte |
| url | https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/66/3/pdf/10.5604_01.3001.0010.4866.pdf |
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