Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production
ABSTRACT: Previous studies have shown that avian hepatitis E virus (HEV) decreases egg production by 10-40% in laying hens, but have not fully elucidated the mechanism of there. In this study, we evaluated the replication of avian HEV in the ovaries of laying hens and the mechanism underlying the de...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2024-04-01
|
Series: | Poultry Science |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124000804 |
_version_ | 1797256478427447296 |
---|---|
author | Yuan Zhang Xueyan Gao Mengdan Cao Huanyu Xu Hanyi Liu Qin Zhao En-Min Zhou Yiyang Chen Baoyuan Liu |
author_facet | Yuan Zhang Xueyan Gao Mengdan Cao Huanyu Xu Hanyi Liu Qin Zhao En-Min Zhou Yiyang Chen Baoyuan Liu |
author_sort | Yuan Zhang |
collection | DOAJ |
description | ABSTRACT: Previous studies have shown that avian hepatitis E virus (HEV) decreases egg production by 10-40% in laying hens, but have not fully elucidated the mechanism of there. In this study, we evaluated the replication of avian HEV in the ovaries of laying hens and the mechanism underlying the decrease in egg production. Forty 150-days-old commercial laying hens were randomly divided into 2 groups of 20 hens each. A total of 1 mL (104GE) of avian HEV stock was inoculated intravenously into each chicken in the experimental group, with 20 chickens in the other group serving as negative controls. Five chickens from each group were necropsied weekly for histopathological examination. The pathogenicity of avian HEV has been characterized by seroconversion, viremia, fecal virus shedding, ovarian lesions, and decreased egg production. Both positive and negative-strand avian HEV RNA, and ORF2 antigens can be detected in the ovaries, suggesting that avian HEV can replicate in the ovaries and serve as an important extrahepatic replication site. The ovaries of laying hens underwent apoptosis after avian HEV infection. These results indicate that avian HEV infection and replication in ovarian tissues cause structural damage to the cells, leading to decreased egg production. |
first_indexed | 2024-03-08T02:02:39Z |
format | Article |
id | doaj.art-5663902af59d4226b2ea101a471d88c5 |
institution | Directory Open Access Journal |
issn | 0032-5791 |
language | English |
last_indexed | 2024-04-24T22:22:23Z |
publishDate | 2024-04-01 |
publisher | Elsevier |
record_format | Article |
series | Poultry Science |
spelling | doaj.art-5663902af59d4226b2ea101a471d88c52024-03-20T06:07:50ZengElsevierPoultry Science0032-57912024-04-011034103501Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg productionYuan Zhang0Xueyan Gao1Mengdan Cao2Huanyu Xu3Hanyi Liu4Qin Zhao5En-Min Zhou6Yiyang Chen7Baoyuan Liu8Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaCorresponding author:; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaABSTRACT: Previous studies have shown that avian hepatitis E virus (HEV) decreases egg production by 10-40% in laying hens, but have not fully elucidated the mechanism of there. In this study, we evaluated the replication of avian HEV in the ovaries of laying hens and the mechanism underlying the decrease in egg production. Forty 150-days-old commercial laying hens were randomly divided into 2 groups of 20 hens each. A total of 1 mL (104GE) of avian HEV stock was inoculated intravenously into each chicken in the experimental group, with 20 chickens in the other group serving as negative controls. Five chickens from each group were necropsied weekly for histopathological examination. The pathogenicity of avian HEV has been characterized by seroconversion, viremia, fecal virus shedding, ovarian lesions, and decreased egg production. Both positive and negative-strand avian HEV RNA, and ORF2 antigens can be detected in the ovaries, suggesting that avian HEV can replicate in the ovaries and serve as an important extrahepatic replication site. The ovaries of laying hens underwent apoptosis after avian HEV infection. These results indicate that avian HEV infection and replication in ovarian tissues cause structural damage to the cells, leading to decreased egg production.http://www.sciencedirect.com/science/article/pii/S0032579124000804hepatitis E viruspathogenicityovaryapoptosis |
spellingShingle | Yuan Zhang Xueyan Gao Mengdan Cao Huanyu Xu Hanyi Liu Qin Zhao En-Min Zhou Yiyang Chen Baoyuan Liu Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production Poultry Science hepatitis E virus pathogenicity ovary apoptosis |
title | Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production |
title_full | Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production |
title_fullStr | Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production |
title_full_unstemmed | Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production |
title_short | Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production |
title_sort | hepatitis e virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production |
topic | hepatitis E virus pathogenicity ovary apoptosis |
url | http://www.sciencedirect.com/science/article/pii/S0032579124000804 |
work_keys_str_mv | AT yuanzhang hepatitiseviruscausesapoptosisofovariancellsinhensandresultinginadecreaseineggproduction AT xueyangao hepatitiseviruscausesapoptosisofovariancellsinhensandresultinginadecreaseineggproduction AT mengdancao hepatitiseviruscausesapoptosisofovariancellsinhensandresultinginadecreaseineggproduction AT huanyuxu hepatitiseviruscausesapoptosisofovariancellsinhensandresultinginadecreaseineggproduction AT hanyiliu hepatitiseviruscausesapoptosisofovariancellsinhensandresultinginadecreaseineggproduction AT qinzhao hepatitiseviruscausesapoptosisofovariancellsinhensandresultinginadecreaseineggproduction AT enminzhou hepatitiseviruscausesapoptosisofovariancellsinhensandresultinginadecreaseineggproduction AT yiyangchen hepatitiseviruscausesapoptosisofovariancellsinhensandresultinginadecreaseineggproduction AT baoyuanliu hepatitiseviruscausesapoptosisofovariancellsinhensandresultinginadecreaseineggproduction |