Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production

ABSTRACT: Previous studies have shown that avian hepatitis E virus (HEV) decreases egg production by 10-40% in laying hens, but have not fully elucidated the mechanism of there. In this study, we evaluated the replication of avian HEV in the ovaries of laying hens and the mechanism underlying the de...

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Main Authors: Yuan Zhang, Xueyan Gao, Mengdan Cao, Huanyu Xu, Hanyi Liu, Qin Zhao, En-Min Zhou, Yiyang Chen, Baoyuan Liu
Format: Article
Language:English
Published: Elsevier 2024-04-01
Series:Poultry Science
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0032579124000804
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author Yuan Zhang
Xueyan Gao
Mengdan Cao
Huanyu Xu
Hanyi Liu
Qin Zhao
En-Min Zhou
Yiyang Chen
Baoyuan Liu
author_facet Yuan Zhang
Xueyan Gao
Mengdan Cao
Huanyu Xu
Hanyi Liu
Qin Zhao
En-Min Zhou
Yiyang Chen
Baoyuan Liu
author_sort Yuan Zhang
collection DOAJ
description ABSTRACT: Previous studies have shown that avian hepatitis E virus (HEV) decreases egg production by 10-40% in laying hens, but have not fully elucidated the mechanism of there. In this study, we evaluated the replication of avian HEV in the ovaries of laying hens and the mechanism underlying the decrease in egg production. Forty 150-days-old commercial laying hens were randomly divided into 2 groups of 20 hens each. A total of 1 mL (104GE) of avian HEV stock was inoculated intravenously into each chicken in the experimental group, with 20 chickens in the other group serving as negative controls. Five chickens from each group were necropsied weekly for histopathological examination. The pathogenicity of avian HEV has been characterized by seroconversion, viremia, fecal virus shedding, ovarian lesions, and decreased egg production. Both positive and negative-strand avian HEV RNA, and ORF2 antigens can be detected in the ovaries, suggesting that avian HEV can replicate in the ovaries and serve as an important extrahepatic replication site. The ovaries of laying hens underwent apoptosis after avian HEV infection. These results indicate that avian HEV infection and replication in ovarian tissues cause structural damage to the cells, leading to decreased egg production.
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spelling doaj.art-5663902af59d4226b2ea101a471d88c52024-03-20T06:07:50ZengElsevierPoultry Science0032-57912024-04-011034103501Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg productionYuan Zhang0Xueyan Gao1Mengdan Cao2Huanyu Xu3Hanyi Liu4Qin Zhao5En-Min Zhou6Yiyang Chen7Baoyuan Liu8Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaCorresponding author:; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaABSTRACT: Previous studies have shown that avian hepatitis E virus (HEV) decreases egg production by 10-40% in laying hens, but have not fully elucidated the mechanism of there. In this study, we evaluated the replication of avian HEV in the ovaries of laying hens and the mechanism underlying the decrease in egg production. Forty 150-days-old commercial laying hens were randomly divided into 2 groups of 20 hens each. A total of 1 mL (104GE) of avian HEV stock was inoculated intravenously into each chicken in the experimental group, with 20 chickens in the other group serving as negative controls. Five chickens from each group were necropsied weekly for histopathological examination. The pathogenicity of avian HEV has been characterized by seroconversion, viremia, fecal virus shedding, ovarian lesions, and decreased egg production. Both positive and negative-strand avian HEV RNA, and ORF2 antigens can be detected in the ovaries, suggesting that avian HEV can replicate in the ovaries and serve as an important extrahepatic replication site. The ovaries of laying hens underwent apoptosis after avian HEV infection. These results indicate that avian HEV infection and replication in ovarian tissues cause structural damage to the cells, leading to decreased egg production.http://www.sciencedirect.com/science/article/pii/S0032579124000804hepatitis E viruspathogenicityovaryapoptosis
spellingShingle Yuan Zhang
Xueyan Gao
Mengdan Cao
Huanyu Xu
Hanyi Liu
Qin Zhao
En-Min Zhou
Yiyang Chen
Baoyuan Liu
Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production
Poultry Science
hepatitis E virus
pathogenicity
ovary
apoptosis
title Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production
title_full Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production
title_fullStr Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production
title_full_unstemmed Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production
title_short Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production
title_sort hepatitis e virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production
topic hepatitis E virus
pathogenicity
ovary
apoptosis
url http://www.sciencedirect.com/science/article/pii/S0032579124000804
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