Development of Electrospun Chitosan-Polyethylene Oxide/Fibrinogen Biocomposite for Potential Wound Healing Applications
Abstract Normal wound healing is a highly complex process that requires the interplay of various growth factors and cell types. Despite advancements in biomaterials, only a few bioactive wound dressings reach the clinical setting. The purpose of this research was to explore the feasibility of electr...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2018-04-01
|
| Series: | Nanoscale Research Letters |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s11671-018-2491-8 |
| _version_ | 1797706784647938048 |
|---|---|
| author | Tony T. Yuan Ann Marie DiGeorge Foushee Monica C. Johnson Angela R. Jockheck-Clark Jonathan M. Stahl |
| author_facet | Tony T. Yuan Ann Marie DiGeorge Foushee Monica C. Johnson Angela R. Jockheck-Clark Jonathan M. Stahl |
| author_sort | Tony T. Yuan |
| collection | DOAJ |
| description | Abstract Normal wound healing is a highly complex process that requires the interplay of various growth factors and cell types. Despite advancements in biomaterials, only a few bioactive wound dressings reach the clinical setting. The purpose of this research was to explore the feasibility of electrospinning a novel nanofibrous chitosan (CS)-fibrinogen (Fb) scaffold capable of sustained release of platelet-derived growth factor (PDGF) for the promotion of fibroblast migration and wound healing. CS-Fb scaffolds were successfully electrospun using a dual-spinneret electrospinner and directly evaluated for their physical, chemical, and biological characteristics. CS-polyethylene/Fb scaffolds exhibited thinner fiber diameters than nanofibers electrospun from the individual components while demonstrating adequate mechanical properties and homogeneous polymer distribution. In addition, the scaffold demonstrated acceptable water transfer rates for wound healing applications. PDGF was successfully incorporated in the scaffold and maintained functional activity throughout the electrospinning process. Furthermore, released PDGF was effective at promoting fibroblast migration equivalent to a single 50 ng/mL dose of PDGF. The current study demonstrates that PDGF-loaded CS-Fb nanofibrous scaffolds possess characteristics that would be highly beneficial as novel bioactive dressings for enhancement of wound healing. |
| first_indexed | 2024-03-12T05:57:26Z |
| format | Article |
| id | doaj.art-566d8f63982b483c9d6b958a5cd4c688 |
| institution | Directory Open Access Journal |
| issn | 1931-7573 1556-276X |
| language | English |
| last_indexed | 2024-03-12T05:57:26Z |
| publishDate | 2018-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-566d8f63982b483c9d6b958a5cd4c6882023-09-03T04:26:45ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2018-04-0113111210.1186/s11671-018-2491-8Development of Electrospun Chitosan-Polyethylene Oxide/Fibrinogen Biocomposite for Potential Wound Healing ApplicationsTony T. Yuan0Ann Marie DiGeorge Foushee1Monica C. Johnson2Angela R. Jockheck-Clark3Jonathan M. Stahl4Naval Medical Research Unit San AntonioNaval Medical Research Unit San AntonioNaval Medical Research Unit San AntonioNaval Medical Research Unit San AntonioNaval Medical Research Unit San AntonioAbstract Normal wound healing is a highly complex process that requires the interplay of various growth factors and cell types. Despite advancements in biomaterials, only a few bioactive wound dressings reach the clinical setting. The purpose of this research was to explore the feasibility of electrospinning a novel nanofibrous chitosan (CS)-fibrinogen (Fb) scaffold capable of sustained release of platelet-derived growth factor (PDGF) for the promotion of fibroblast migration and wound healing. CS-Fb scaffolds were successfully electrospun using a dual-spinneret electrospinner and directly evaluated for their physical, chemical, and biological characteristics. CS-polyethylene/Fb scaffolds exhibited thinner fiber diameters than nanofibers electrospun from the individual components while demonstrating adequate mechanical properties and homogeneous polymer distribution. In addition, the scaffold demonstrated acceptable water transfer rates for wound healing applications. PDGF was successfully incorporated in the scaffold and maintained functional activity throughout the electrospinning process. Furthermore, released PDGF was effective at promoting fibroblast migration equivalent to a single 50 ng/mL dose of PDGF. The current study demonstrates that PDGF-loaded CS-Fb nanofibrous scaffolds possess characteristics that would be highly beneficial as novel bioactive dressings for enhancement of wound healing.http://link.springer.com/article/10.1186/s11671-018-2491-8ElectrospinningBiomedical applicationsChitosanWound dressingFibrinogenNanofibers |
| spellingShingle | Tony T. Yuan Ann Marie DiGeorge Foushee Monica C. Johnson Angela R. Jockheck-Clark Jonathan M. Stahl Development of Electrospun Chitosan-Polyethylene Oxide/Fibrinogen Biocomposite for Potential Wound Healing Applications Nanoscale Research Letters Electrospinning Biomedical applications Chitosan Wound dressing Fibrinogen Nanofibers |
| title | Development of Electrospun Chitosan-Polyethylene Oxide/Fibrinogen Biocomposite for Potential Wound Healing Applications |
| title_full | Development of Electrospun Chitosan-Polyethylene Oxide/Fibrinogen Biocomposite for Potential Wound Healing Applications |
| title_fullStr | Development of Electrospun Chitosan-Polyethylene Oxide/Fibrinogen Biocomposite for Potential Wound Healing Applications |
| title_full_unstemmed | Development of Electrospun Chitosan-Polyethylene Oxide/Fibrinogen Biocomposite for Potential Wound Healing Applications |
| title_short | Development of Electrospun Chitosan-Polyethylene Oxide/Fibrinogen Biocomposite for Potential Wound Healing Applications |
| title_sort | development of electrospun chitosan polyethylene oxide fibrinogen biocomposite for potential wound healing applications |
| topic | Electrospinning Biomedical applications Chitosan Wound dressing Fibrinogen Nanofibers |
| url | http://link.springer.com/article/10.1186/s11671-018-2491-8 |
| work_keys_str_mv | AT tonytyuan developmentofelectrospunchitosanpolyethyleneoxidefibrinogenbiocompositeforpotentialwoundhealingapplications AT annmariedigeorgefoushee developmentofelectrospunchitosanpolyethyleneoxidefibrinogenbiocompositeforpotentialwoundhealingapplications AT monicacjohnson developmentofelectrospunchitosanpolyethyleneoxidefibrinogenbiocompositeforpotentialwoundhealingapplications AT angelarjockheckclark developmentofelectrospunchitosanpolyethyleneoxidefibrinogenbiocompositeforpotentialwoundhealingapplications AT jonathanmstahl developmentofelectrospunchitosanpolyethyleneoxidefibrinogenbiocompositeforpotentialwoundhealingapplications |