Simultaneous determination of 8 bufo toxins in toad skin by UPLC/Q-TOF-MS
ObjectiveTo establish a rapid qualitative and quantitative method for the determination of 8 bufo toxins in toad skin residual from poisoned patients by ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry （UPLC/Q-TOF-MS）.MethodsAfter the toad skin samples were...
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2023-04-01
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| Series: | Zhongguo shipin weisheng zazhi |
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| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/202304008 |
| _version_ | 1827907614100422656 |
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| author | MIAO Qionghui CHEN Rumei HUANG Xiaoqin LIN Ji |
| author_facet | MIAO Qionghui CHEN Rumei HUANG Xiaoqin LIN Ji |
| author_sort | MIAO Qionghui |
| collection | DOAJ |
| description | ObjectiveTo establish a rapid qualitative and quantitative method for the determination of 8 bufo toxins in toad skin residual from poisoned patients by ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry （UPLC/Q-TOF-MS）.MethodsAfter the toad skin samples were pretreated， the sample solution was separated on A Cquity UPLC® HSS T3 column （100 mm×2.1 mm， 1.8 μm） with 0.1% formic acid solution as mobile phase A and methanol as mobile phase B. The flow rate was 0.3 mL/min， the column temperature was 35 ℃， and the sample volume was 2 μL.ResultsThe linear ranges were 10.0-1 000.0 μg/L with r2≥0.990. The limits of detection were 0.7-2.1 μg/kg， and the limits of quantification were 2.5-7.0 μg/kg. The average recoveries were ≥75.79% and RSDs were ≤9.84%. The contents of bufalin， desacetylcinobufagin， desacetylcinobufotalin， pseudobufarenogin and bufotaline were 123.8±3.1， 5 920.4±1.9， 680.7±3.2， 671.6±9.1 and 1 796.6±74.9 μg/kg， respectively， while gamabufotain， cinobufagin and cinobufotalin were not detected.ConclusionThe method is practical， rapid and reliable for the determination of cinobufagin， bufalin， pseudobufarenogin， desacetylcinobufotalin and desacetylcinobufagin， bufotaline， gamabufotain and cinobufotalin in toad skin. |
| first_indexed | 2024-03-13T01:10:35Z |
| format | Article |
| id | doaj.art-566e4bda941447a58b3fc8b8b6e8648c |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-03-13T01:10:35Z |
| publishDate | 2023-04-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-566e4bda941447a58b3fc8b8b6e8648c2023-07-06T02:43:43ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562023-04-0135453554210.13590/j.cjfh.2023.04.0081004-8456（2023）04-0535-08Simultaneous determination of 8 bufo toxins in toad skin by UPLC/Q-TOF-MSMIAO Qionghui0CHEN Rumei1HUANG Xiaoqin2LIN Ji3Dali University, Yunnan Dali 671000, ChinaKunming Medical University, Yunnan Kunming 650500, ChinaKunming Medical University, Yunnan Kunming 650500, ChinaYunnan Center for Disease Control and Prevention, Yunnan Kunming 650032, ChinaObjectiveTo establish a rapid qualitative and quantitative method for the determination of 8 bufo toxins in toad skin residual from poisoned patients by ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry （UPLC/Q-TOF-MS）.MethodsAfter the toad skin samples were pretreated， the sample solution was separated on A Cquity UPLC® HSS T3 column （100 mm×2.1 mm， 1.8 μm） with 0.1% formic acid solution as mobile phase A and methanol as mobile phase B. The flow rate was 0.3 mL/min， the column temperature was 35 ℃， and the sample volume was 2 μL.ResultsThe linear ranges were 10.0-1 000.0 μg/L with r2≥0.990. The limits of detection were 0.7-2.1 μg/kg， and the limits of quantification were 2.5-7.0 μg/kg. The average recoveries were ≥75.79% and RSDs were ≤9.84%. The contents of bufalin， desacetylcinobufagin， desacetylcinobufotalin， pseudobufarenogin and bufotaline were 123.8±3.1， 5 920.4±1.9， 680.7±3.2， 671.6±9.1 and 1 796.6±74.9 μg/kg， respectively， while gamabufotain， cinobufagin and cinobufotalin were not detected.ConclusionThe method is practical， rapid and reliable for the determination of cinobufagin， bufalin， pseudobufarenogin， desacetylcinobufotalin and desacetylcinobufagin， bufotaline， gamabufotain and cinobufotalin in toad skin.http://www.zgspws.com/zgspwszz/article/abstract/202304008ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometrybufo toxinsimultaneous determination |
| spellingShingle | MIAO Qionghui CHEN Rumei HUANG Xiaoqin LIN Ji Simultaneous determination of 8 bufo toxins in toad skin by UPLC/Q-TOF-MS Zhongguo shipin weisheng zazhi ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry bufo toxin simultaneous determination |
| title | Simultaneous determination of 8 bufo toxins in toad skin by UPLC/Q-TOF-MS |
| title_full | Simultaneous determination of 8 bufo toxins in toad skin by UPLC/Q-TOF-MS |
| title_fullStr | Simultaneous determination of 8 bufo toxins in toad skin by UPLC/Q-TOF-MS |
| title_full_unstemmed | Simultaneous determination of 8 bufo toxins in toad skin by UPLC/Q-TOF-MS |
| title_short | Simultaneous determination of 8 bufo toxins in toad skin by UPLC/Q-TOF-MS |
| title_sort | simultaneous determination of 8 bufo toxins in toad skin by uplc q tof ms |
| topic | ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry bufo toxin simultaneous determination |
| url | http://www.zgspws.com/zgspwszz/article/abstract/202304008 |
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