Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV
Abstract Barley yellow dwarf viruses (BYDVs) cause significant economic losses on barley, wheat, and oats worldwide. 17-kDa protein (17K) of BYDVs plays a key role in viral infection in plants, whereas the underlying regulation mechanism of 17K in virus infection remains elusive. In this study, we d...
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| Format: | Article |
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Nature Portfolio
2021-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-87836-1 |
| _version_ | 1818425394970230784 |
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| author | Siyu Chen Xiaoyu Han Lingling Yang Qinglun Li Yajuan Shi Honglian Li Linlin Chen Bingjian Sun Yan Shi Xue Yang |
| author_facet | Siyu Chen Xiaoyu Han Lingling Yang Qinglun Li Yajuan Shi Honglian Li Linlin Chen Bingjian Sun Yan Shi Xue Yang |
| author_sort | Siyu Chen |
| collection | DOAJ |
| description | Abstract Barley yellow dwarf viruses (BYDVs) cause significant economic losses on barley, wheat, and oats worldwide. 17-kDa protein (17K) of BYDVs plays a key role in viral infection in plants, whereas the underlying regulation mechanism of 17K in virus infection remains elusive. In this study, we determined that 17K of BYDV-GAV, the most common species found in China in recent years, was involved in viral pathogenicity. To identify the host factors interacting with 17K, the full length coding sequence of 17K was cloned into pGBKT7 to generate the bait plasmid pGBKT7-17K. 114 positive clones were identified as possible host factors to interact with 17K through screening a tobacco cDNA library. Gene ontology enrichment analysis showed that they were classified into 35 functional groups, involving three main categories including biological processes (BP), cellular components (CC), and molecular functions (MF). Kyoto Encyclopedia of Genes and Genome (KEGG) analysis indicated the acquired genes were assigned to 49 KEGG pathways. The majority of these genes were involved in glyoxylate and dicarboxylate metabolism, carbon fixation in photosynthetic organisms, and glycolysis/gluconeogenesis. The interactions between 17K and the 27 proteins with well-documented annotations were verified by conducting yeast two-hybrid assays and 12 of the 27 proteins were verified to interact with 17K. To explore the putative function of the 12 proteins in BYDV-GAV infection, the subcellular localization and expression alterations in the presence of BYDV-GAV were monitored. The results showed that, under the condition of BYDV-GAV infection, RuBisCo, POR, and PPD5 were significantly up-regulated, whereas AEP and CAT1 were significantly down-regulated. Our findings provide insights into the 17K-mediated BYDV-GAV infection process. |
| first_indexed | 2024-12-14T14:13:15Z |
| format | Article |
| id | doaj.art-566f0455e8d04be3aa6645d00b6462ce |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T14:13:15Z |
| publishDate | 2021-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-566f0455e8d04be3aa6645d00b6462ce2022-12-21T22:58:15ZengNature PortfolioScientific Reports2045-23222021-04-0111111110.1038/s41598-021-87836-1Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAVSiyu Chen0Xiaoyu Han1Lingling Yang2Qinglun Li3Yajuan Shi4Honglian Li5Linlin Chen6Bingjian Sun7Yan Shi8Xue Yang9College of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityAbstract Barley yellow dwarf viruses (BYDVs) cause significant economic losses on barley, wheat, and oats worldwide. 17-kDa protein (17K) of BYDVs plays a key role in viral infection in plants, whereas the underlying regulation mechanism of 17K in virus infection remains elusive. In this study, we determined that 17K of BYDV-GAV, the most common species found in China in recent years, was involved in viral pathogenicity. To identify the host factors interacting with 17K, the full length coding sequence of 17K was cloned into pGBKT7 to generate the bait plasmid pGBKT7-17K. 114 positive clones were identified as possible host factors to interact with 17K through screening a tobacco cDNA library. Gene ontology enrichment analysis showed that they were classified into 35 functional groups, involving three main categories including biological processes (BP), cellular components (CC), and molecular functions (MF). Kyoto Encyclopedia of Genes and Genome (KEGG) analysis indicated the acquired genes were assigned to 49 KEGG pathways. The majority of these genes were involved in glyoxylate and dicarboxylate metabolism, carbon fixation in photosynthetic organisms, and glycolysis/gluconeogenesis. The interactions between 17K and the 27 proteins with well-documented annotations were verified by conducting yeast two-hybrid assays and 12 of the 27 proteins were verified to interact with 17K. To explore the putative function of the 12 proteins in BYDV-GAV infection, the subcellular localization and expression alterations in the presence of BYDV-GAV were monitored. The results showed that, under the condition of BYDV-GAV infection, RuBisCo, POR, and PPD5 were significantly up-regulated, whereas AEP and CAT1 were significantly down-regulated. Our findings provide insights into the 17K-mediated BYDV-GAV infection process.https://doi.org/10.1038/s41598-021-87836-1 |
| spellingShingle | Siyu Chen Xiaoyu Han Lingling Yang Qinglun Li Yajuan Shi Honglian Li Linlin Chen Bingjian Sun Yan Shi Xue Yang Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV Scientific Reports |
| title | Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV |
| title_full | Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV |
| title_fullStr | Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV |
| title_full_unstemmed | Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV |
| title_short | Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV |
| title_sort | identification and functional analyses of host factors interacting with the 17 kda protein of barley yellow dwarf virus gav |
| url | https://doi.org/10.1038/s41598-021-87836-1 |
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