Temporal system‐level organization of the switch from glycolytic to gluconeogenic operation in yeast
Abstract The diauxic shift in Saccharomyces cerevisiae is an ideal model to study how eukaryotic cells readjust their metabolism from glycolytic to gluconeogenic operation. In this work, we generated time‐resolved physiological data, quantitative metabolome (69 intracellular metabolites) and proteom...
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| Format: | Article |
| Language: | English |
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Springer Nature
2013-04-01
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| Series: | Molecular Systems Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/msb.2013.11 |
| _version_ | 1826990437398740992 |
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| author | Guillermo G Zampar Anne Kümmel Jennifer Ewald Stefan Jol Bastian Niebel Paola Picotti Ruedi Aebersold Uwe Sauer Nicola Zamboni Matthias Heinemann |
| author_facet | Guillermo G Zampar Anne Kümmel Jennifer Ewald Stefan Jol Bastian Niebel Paola Picotti Ruedi Aebersold Uwe Sauer Nicola Zamboni Matthias Heinemann |
| author_sort | Guillermo G Zampar |
| collection | DOAJ |
| description | Abstract The diauxic shift in Saccharomyces cerevisiae is an ideal model to study how eukaryotic cells readjust their metabolism from glycolytic to gluconeogenic operation. In this work, we generated time‐resolved physiological data, quantitative metabolome (69 intracellular metabolites) and proteome (72 enzymes) profiles. We found that the diauxic shift is accomplished by three key events that are temporally organized: (i) a reduction in the glycolytic flux and the production of storage compounds before glucose depletion, mediated by downregulation of phosphofructokinase and pyruvate kinase reactions; (ii) upon glucose exhaustion, the reversion of carbon flow through glycolysis and onset of the glyoxylate cycle operation triggered by an increased expression of the enzymes that catalyze the malate synthase and cytosolic citrate synthase reactions; and (iii) in the later stages of the adaptation, the shutting down of the pentose phosphate pathway with a change in NADPH regeneration. Moreover, we identified the transcription factors associated with the observed changes in protein abundances. Taken together, our results represent an important contribution toward a systems‐level understanding of how this adaptation is realized. |
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| format | Article |
| id | doaj.art-566f18f09ef34c4988f93c59c7a75b78 |
| institution | Directory Open Access Journal |
| issn | 1744-4292 |
| language | English |
| last_indexed | 2025-02-18T08:20:32Z |
| publishDate | 2013-04-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj.art-566f18f09ef34c4988f93c59c7a75b782024-11-03T12:55:54ZengSpringer NatureMolecular Systems Biology1744-42922013-04-019111310.1038/msb.2013.11Temporal system‐level organization of the switch from glycolytic to gluconeogenic operation in yeastGuillermo G Zampar0Anne Kümmel1Jennifer Ewald2Stefan Jol3Bastian Niebel4Paola Picotti5Ruedi Aebersold6Uwe Sauer7Nicola Zamboni8Matthias Heinemann9Molecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenETH Zurich, Institute of Molecular Systems BiologyETH Zurich, Institute of Molecular Systems BiologyETH Zurich, Institute of Molecular Systems BiologyMolecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenETH Zurich, Institute of Molecular Systems BiologyETH Zurich, Institute of Molecular Systems BiologyETH Zurich, Institute of Molecular Systems BiologyETH Zurich, Institute of Molecular Systems BiologyMolecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenAbstract The diauxic shift in Saccharomyces cerevisiae is an ideal model to study how eukaryotic cells readjust their metabolism from glycolytic to gluconeogenic operation. In this work, we generated time‐resolved physiological data, quantitative metabolome (69 intracellular metabolites) and proteome (72 enzymes) profiles. We found that the diauxic shift is accomplished by three key events that are temporally organized: (i) a reduction in the glycolytic flux and the production of storage compounds before glucose depletion, mediated by downregulation of phosphofructokinase and pyruvate kinase reactions; (ii) upon glucose exhaustion, the reversion of carbon flow through glycolysis and onset of the glyoxylate cycle operation triggered by an increased expression of the enzymes that catalyze the malate synthase and cytosolic citrate synthase reactions; and (iii) in the later stages of the adaptation, the shutting down of the pentose phosphate pathway with a change in NADPH regeneration. Moreover, we identified the transcription factors associated with the observed changes in protein abundances. Taken together, our results represent an important contribution toward a systems‐level understanding of how this adaptation is realized.https://doi.org/10.1038/msb.2013.11diauxic shiftfluxomemetabolomeproteomeSaccharomyces cerevisiae |
| spellingShingle | Guillermo G Zampar Anne Kümmel Jennifer Ewald Stefan Jol Bastian Niebel Paola Picotti Ruedi Aebersold Uwe Sauer Nicola Zamboni Matthias Heinemann Temporal system‐level organization of the switch from glycolytic to gluconeogenic operation in yeast Molecular Systems Biology diauxic shift fluxome metabolome proteome Saccharomyces cerevisiae |
| title | Temporal system‐level organization of the switch from glycolytic to gluconeogenic operation in yeast |
| title_full | Temporal system‐level organization of the switch from glycolytic to gluconeogenic operation in yeast |
| title_fullStr | Temporal system‐level organization of the switch from glycolytic to gluconeogenic operation in yeast |
| title_full_unstemmed | Temporal system‐level organization of the switch from glycolytic to gluconeogenic operation in yeast |
| title_short | Temporal system‐level organization of the switch from glycolytic to gluconeogenic operation in yeast |
| title_sort | temporal system level organization of the switch from glycolytic to gluconeogenic operation in yeast |
| topic | diauxic shift fluxome metabolome proteome Saccharomyces cerevisiae |
| url | https://doi.org/10.1038/msb.2013.11 |
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