ERYTHROCYTES OF SKIERS-RACERS OF MEN AND WOMEN IN THE ANNUAL CYCLE
Purpose. Determining the gender characteristics of the morphofunctional state of red blood in athletes within 10 months of annual monitoring Materials and methods. The morphofunctional composition of red blood was studied from highly qualified athletes from the first category to the masters of spor...
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| Format: | Article |
| Language: | English |
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Science and Innovation Center Publishing House
2019-05-01
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| Series: | Siberian Journal of Life Sciences and Agriculture |
| Subjects: | |
| Online Access: | http://journal-s.org/index.php/vmno/article/view/12357 |
| _version_ | 1818148375065788416 |
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| author | Nikolay Petrovich Mongalev Lidiya Yurevna Rubtsova Natalya Nikolaevna Potolitsyna |
| author_facet | Nikolay Petrovich Mongalev Lidiya Yurevna Rubtsova Natalya Nikolaevna Potolitsyna |
| author_sort | Nikolay Petrovich Mongalev |
| collection | DOAJ |
| description | Purpose. Determining the gender characteristics of the morphofunctional state of red blood in athletes within 10 months of annual monitoring
Materials and methods. The morphofunctional composition of red blood was studied from highly qualified athletes from the first category to the masters of sports in ski racing, members of the Komi Republic national teams – men
(n = 87) aged 23.1 ± 0.57 and women (n = 62) 20.9 ± 0.61 years for 10 months of the annual cycle. The study was conducted according to the protocol approved by the local bioethics committee of the Federal Research Center “Komi Scientific Center of Ural Branch of the Russian Academy of Sciences”. Athletes in the morning on an empty stomach produced background blood sampling from the cubital vein to vacutainers (England). On blood smears stained by Romanovsky-Giemsa (Vital-Development), diamond cresyl blue (Russia) measured the diameter of 50 red blood cells and reticulocytes using an MB microscope with oil immersion, an increase of about. 100x approx. with a graduated scale of 12x. The hematocrit was determined using a MPW-310 centrifuge (Poland), hemoglobin by the unified hemiglobin cyanide method (REF) (Russia), the red blood cell count was calculated using a Goryaev camera in accordance with the requirements of a clinical and hematological examination. The results were processed by parametric statistics using the Excel and Statistica for Windows 6.0. Application licensed programs, which are presented as arithmetic mean (M), mean error (m). To compare the differences, Student’s t-test was used with a significance level from 0.05 to 0.001.
Results. The presence of statistically significant differences in highly qualified athletes between the indicators of men and women in the annual cycle in hematocrit, hemoglobin, red blood cell count, volume, red blood cell diameter and surface, including in a liter of blood (p <0.001). Gender features were revealed in the nature of changes in the cellular composition of red blood by the size of erythrocytes stained in vitro with vital dyes (p <0.05), diameter and number of reticulocytes, hemoglobin concentration in erythrocytes (p <0.001).
Conclusion. Monitoring of gender-based racers showed that the existing differences in the morphofunctional state of red blood in men and women in the annual cycle persisted, which may indicate an adequate process of training athletes to the level of high qualification. |
| first_indexed | 2024-12-11T12:50:08Z |
| format | Article |
| id | doaj.art-5670004bf9564317a9bca5bdbad7bdc8 |
| institution | Directory Open Access Journal |
| issn | 2658-6649 2658-6657 |
| language | English |
| last_indexed | 2024-12-11T12:50:08Z |
| publishDate | 2019-05-01 |
| publisher | Science and Innovation Center Publishing House |
| record_format | Article |
| series | Siberian Journal of Life Sciences and Agriculture |
| spelling | doaj.art-5670004bf9564317a9bca5bdbad7bdc82022-12-22T01:06:42ZengScience and Innovation Center Publishing HouseSiberian Journal of Life Sciences and Agriculture2658-66492658-66572019-05-01112244510.12731/2658-6649-2019-11-2-24-457585ERYTHROCYTES OF SKIERS-RACERS OF MEN AND WOMEN IN THE ANNUAL CYCLENikolay Petrovich Mongalev0Lidiya Yurevna Rubtsova1Natalya Nikolaevna Potolitsyna2Институт физиологии Коми научного центра Уральского отделения Российской академии наук, ФИЦ Коми НЦ УрО РАНИнститут физиологии Коми научного центра Уральского отделения Российской академии наук, ФИЦ Коми НЦ УрО РАНИнститут физиологии Коми научного центра Уральского отделения Российской академии наук, ФИЦ Коми НЦ УрО РАНPurpose. Determining the gender characteristics of the morphofunctional state of red blood in athletes within 10 months of annual monitoring Materials and methods. The morphofunctional composition of red blood was studied from highly qualified athletes from the first category to the masters of sports in ski racing, members of the Komi Republic national teams – men (n = 87) aged 23.1 ± 0.57 and women (n = 62) 20.9 ± 0.61 years for 10 months of the annual cycle. The study was conducted according to the protocol approved by the local bioethics committee of the Federal Research Center “Komi Scientific Center of Ural Branch of the Russian Academy of Sciences”. Athletes in the morning on an empty stomach produced background blood sampling from the cubital vein to vacutainers (England). On blood smears stained by Romanovsky-Giemsa (Vital-Development), diamond cresyl blue (Russia) measured the diameter of 50 red blood cells and reticulocytes using an MB microscope with oil immersion, an increase of about. 100x approx. with a graduated scale of 12x. The hematocrit was determined using a MPW-310 centrifuge (Poland), hemoglobin by the unified hemiglobin cyanide method (REF) (Russia), the red blood cell count was calculated using a Goryaev camera in accordance with the requirements of a clinical and hematological examination. The results were processed by parametric statistics using the Excel and Statistica for Windows 6.0. Application licensed programs, which are presented as arithmetic mean (M), mean error (m). To compare the differences, Student’s t-test was used with a significance level from 0.05 to 0.001. Results. The presence of statistically significant differences in highly qualified athletes between the indicators of men and women in the annual cycle in hematocrit, hemoglobin, red blood cell count, volume, red blood cell diameter and surface, including in a liter of blood (p <0.001). Gender features were revealed in the nature of changes in the cellular composition of red blood by the size of erythrocytes stained in vitro with vital dyes (p <0.05), diameter and number of reticulocytes, hemoglobin concentration in erythrocytes (p <0.001). Conclusion. Monitoring of gender-based racers showed that the existing differences in the morphofunctional state of red blood in men and women in the annual cycle persisted, which may indicate an adequate process of training athletes to the level of high qualification.http://journal-s.org/index.php/vmno/article/view/12357красная кровьконцентрация гемоглобина в эритроцитеспортсменымужчиныженщиныгодовой цикл |
| spellingShingle | Nikolay Petrovich Mongalev Lidiya Yurevna Rubtsova Natalya Nikolaevna Potolitsyna ERYTHROCYTES OF SKIERS-RACERS OF MEN AND WOMEN IN THE ANNUAL CYCLE Siberian Journal of Life Sciences and Agriculture красная кровь концентрация гемоглобина в эритроците спортсмены мужчины женщины годовой цикл |
| title | ERYTHROCYTES OF SKIERS-RACERS OF MEN AND WOMEN IN THE ANNUAL CYCLE |
| title_full | ERYTHROCYTES OF SKIERS-RACERS OF MEN AND WOMEN IN THE ANNUAL CYCLE |
| title_fullStr | ERYTHROCYTES OF SKIERS-RACERS OF MEN AND WOMEN IN THE ANNUAL CYCLE |
| title_full_unstemmed | ERYTHROCYTES OF SKIERS-RACERS OF MEN AND WOMEN IN THE ANNUAL CYCLE |
| title_short | ERYTHROCYTES OF SKIERS-RACERS OF MEN AND WOMEN IN THE ANNUAL CYCLE |
| title_sort | erythrocytes of skiers racers of men and women in the annual cycle |
| topic | красная кровь концентрация гемоглобина в эритроците спортсмены мужчины женщины годовой цикл |
| url | http://journal-s.org/index.php/vmno/article/view/12357 |
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