Inulin accelerates weight loss in obese mice by regulating gut microbiota and serum metabolites
Several studies indicated that the gut microbiota might participate in the beneficial effect of inulin on obesity. However, the mechanisms involved were still largely unknown. Sixteen high-fat diets (HFDs)-induced obese C57BL/6 mice were converted to a normal diet and then randomized into two groups...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.980382/full |
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| author | Zeang Wu Zhenzhu Du Yuanyuan Tian Miao Liu Kailong Zhu Yufan Zhao Haixia Wang |
| author_facet | Zeang Wu Zhenzhu Du Yuanyuan Tian Miao Liu Kailong Zhu Yufan Zhao Haixia Wang |
| author_sort | Zeang Wu |
| collection | DOAJ |
| description | Several studies indicated that the gut microbiota might participate in the beneficial effect of inulin on obesity. However, the mechanisms involved were still largely unknown. Sixteen high-fat diets (HFDs)-induced obese C57BL/6 mice were converted to a normal diet and then randomized into two groups, OND (obese mice + normal diet) group gavage-fed for 10 weeks with normal saline and ONDI (obese mice + normal diet + inulin) group with inulin at 10 g/kg/day. The body weight of HFD-induced obese mice showed different degrees of decrease in both groups. However, the ONDI group lost more weight and returned to normal earlier. Compared to the OND group, inulin supplementation significantly shifted the composition and structure of gut microbiota, such as higher α diversity. The β diversity analysis also confirmed the changes in gut microbiota composition between groups. At the genus level, the abundance of Alistipes was considerably increased, and it was significantly correlated with inulin supplementation (r = 0.72, P = 0.002). Serum metabolite levels were distinctly altered after inulin supplementation, and 143 metabolites were significantly altered in the ONDI group. Among them, indole-3-acrylic acid level increased more than 500-fold compared to the OND group. It was also strongly positive correlation with Alistipes (r = 0.72, P = 0.002) and inulin supplementation (r = 0.99, P = 9.2e−13) and negatively correlated with obesity (r = −0.72, P = 0.002). In conclusion, inulin supplementation could accelerate body weight loss in obese mice by increasing Alistipes and indole-3-acrylic acid level. |
| first_indexed | 2024-12-10T06:14:52Z |
| format | Article |
| id | doaj.art-56783559b0c14678972ddbcc8af27881 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-10T06:14:52Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-56783559b0c14678972ddbcc8af278812022-12-22T01:59:28ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-09-01910.3389/fnut.2022.980382980382Inulin accelerates weight loss in obese mice by regulating gut microbiota and serum metabolitesZeang Wu0Zhenzhu Du1Yuanyuan Tian2Miao Liu3Kailong Zhu4Yufan Zhao5Haixia Wang6First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, ChinaAnalysis and Testing Center, Shihezi University, Shihezi, ChinaSchool of Medicine, Shihezi University, Shihezi, ChinaSchool of Medicine, Shihezi University, Shihezi, ChinaSchool of Medicine, Shihezi University, Shihezi, ChinaSchool of Medicine, Shihezi University, Shihezi, ChinaSchool of Medicine, Shihezi University, Shihezi, ChinaSeveral studies indicated that the gut microbiota might participate in the beneficial effect of inulin on obesity. However, the mechanisms involved were still largely unknown. Sixteen high-fat diets (HFDs)-induced obese C57BL/6 mice were converted to a normal diet and then randomized into two groups, OND (obese mice + normal diet) group gavage-fed for 10 weeks with normal saline and ONDI (obese mice + normal diet + inulin) group with inulin at 10 g/kg/day. The body weight of HFD-induced obese mice showed different degrees of decrease in both groups. However, the ONDI group lost more weight and returned to normal earlier. Compared to the OND group, inulin supplementation significantly shifted the composition and structure of gut microbiota, such as higher α diversity. The β diversity analysis also confirmed the changes in gut microbiota composition between groups. At the genus level, the abundance of Alistipes was considerably increased, and it was significantly correlated with inulin supplementation (r = 0.72, P = 0.002). Serum metabolite levels were distinctly altered after inulin supplementation, and 143 metabolites were significantly altered in the ONDI group. Among them, indole-3-acrylic acid level increased more than 500-fold compared to the OND group. It was also strongly positive correlation with Alistipes (r = 0.72, P = 0.002) and inulin supplementation (r = 0.99, P = 9.2e−13) and negatively correlated with obesity (r = −0.72, P = 0.002). In conclusion, inulin supplementation could accelerate body weight loss in obese mice by increasing Alistipes and indole-3-acrylic acid level.https://www.frontiersin.org/articles/10.3389/fnut.2022.980382/fullobesityinulingut microbiotaAlistipesindole-3-acrylic acid |
| spellingShingle | Zeang Wu Zhenzhu Du Yuanyuan Tian Miao Liu Kailong Zhu Yufan Zhao Haixia Wang Inulin accelerates weight loss in obese mice by regulating gut microbiota and serum metabolites Frontiers in Nutrition obesity inulin gut microbiota Alistipes indole-3-acrylic acid |
| title | Inulin accelerates weight loss in obese mice by regulating gut microbiota and serum metabolites |
| title_full | Inulin accelerates weight loss in obese mice by regulating gut microbiota and serum metabolites |
| title_fullStr | Inulin accelerates weight loss in obese mice by regulating gut microbiota and serum metabolites |
| title_full_unstemmed | Inulin accelerates weight loss in obese mice by regulating gut microbiota and serum metabolites |
| title_short | Inulin accelerates weight loss in obese mice by regulating gut microbiota and serum metabolites |
| title_sort | inulin accelerates weight loss in obese mice by regulating gut microbiota and serum metabolites |
| topic | obesity inulin gut microbiota Alistipes indole-3-acrylic acid |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.980382/full |
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