A New Subfamily of Glycoside Hydrolase Family 30 with Strict Xylobiohydrolase Function
The Acetivibrioclariflavus (basonym: Clostridium clariflavum) glycoside hydrolase family 30 cellulosomal protein encoded by the Clocl_1795 gene was highly represented during growth on cellulosic substrates. In this report, the recombinantly expressed protein has been characterized and shown to be a...
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| Format: | Article |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2021.714238/full |
| _version_ | 1819016039481999360 |
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| author | Casey Crooks Nathan J. Bechle Franz J. St John |
| author_facet | Casey Crooks Nathan J. Bechle Franz J. St John |
| author_sort | Casey Crooks |
| collection | DOAJ |
| description | The Acetivibrioclariflavus (basonym: Clostridium clariflavum) glycoside hydrolase family 30 cellulosomal protein encoded by the Clocl_1795 gene was highly represented during growth on cellulosic substrates. In this report, the recombinantly expressed protein has been characterized and shown to be a non-reducing terminal (NRT)-specific xylobiohydrolase (AcXbh30A). Biochemical function, optimal biophysical parameters, and phylogeny were investigated. The findings indicate that AcXbh30A strictly cleaves xylobiose from the NRT up until an α-1,2-linked glucuronic acid (GA)-decorated xylose if the number of xyloses is even or otherwise a single xylose will remain resulting in a penultimate GA-substituted xylose. Unlike recently reported xylobiohydrolases, AcXbh30A has no other detectable hydrolysis products under our optimized reaction conditions. Sequence analysis indicates that AcXbh30A represents a new GH30 subfamily. This new xylobiohydrolase may be useful for commercial production of industrial quantities of xylobiose. |
| first_indexed | 2024-12-21T02:41:17Z |
| format | Article |
| id | doaj.art-5679047f5b9a4d76afdf8347f6136b23 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-21T02:41:17Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-5679047f5b9a4d76afdf8347f6136b232022-12-21T19:18:40ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2021-09-01810.3389/fmolb.2021.714238714238A New Subfamily of Glycoside Hydrolase Family 30 with Strict Xylobiohydrolase FunctionCasey Crooks0Nathan J. Bechle1Franz J. St John2Institute for Microbial and Biochemical Technology, Forest Products Laboratory, USDA Forest Service, Madison, WI, United StatesEngineering Mechanics and Remote Sensing Laboratory, Forest Products Laboratory, USDA Forest Service, Madison, WI, United StatesInstitute for Microbial and Biochemical Technology, Forest Products Laboratory, USDA Forest Service, Madison, WI, United StatesThe Acetivibrioclariflavus (basonym: Clostridium clariflavum) glycoside hydrolase family 30 cellulosomal protein encoded by the Clocl_1795 gene was highly represented during growth on cellulosic substrates. In this report, the recombinantly expressed protein has been characterized and shown to be a non-reducing terminal (NRT)-specific xylobiohydrolase (AcXbh30A). Biochemical function, optimal biophysical parameters, and phylogeny were investigated. The findings indicate that AcXbh30A strictly cleaves xylobiose from the NRT up until an α-1,2-linked glucuronic acid (GA)-decorated xylose if the number of xyloses is even or otherwise a single xylose will remain resulting in a penultimate GA-substituted xylose. Unlike recently reported xylobiohydrolases, AcXbh30A has no other detectable hydrolysis products under our optimized reaction conditions. Sequence analysis indicates that AcXbh30A represents a new GH30 subfamily. This new xylobiohydrolase may be useful for commercial production of industrial quantities of xylobiose.https://www.frontiersin.org/articles/10.3389/fmolb.2021.714238/fullxylobiohydrolaseglycoside hydrolaseglycoside hydrolase family 30AcXbh30AAcetivibrio clariflavusxylobiose |
| spellingShingle | Casey Crooks Nathan J. Bechle Franz J. St John A New Subfamily of Glycoside Hydrolase Family 30 with Strict Xylobiohydrolase Function Frontiers in Molecular Biosciences xylobiohydrolase glycoside hydrolase glycoside hydrolase family 30 AcXbh30A Acetivibrio clariflavus xylobiose |
| title | A New Subfamily of Glycoside Hydrolase Family 30 with Strict Xylobiohydrolase Function |
| title_full | A New Subfamily of Glycoside Hydrolase Family 30 with Strict Xylobiohydrolase Function |
| title_fullStr | A New Subfamily of Glycoside Hydrolase Family 30 with Strict Xylobiohydrolase Function |
| title_full_unstemmed | A New Subfamily of Glycoside Hydrolase Family 30 with Strict Xylobiohydrolase Function |
| title_short | A New Subfamily of Glycoside Hydrolase Family 30 with Strict Xylobiohydrolase Function |
| title_sort | new subfamily of glycoside hydrolase family 30 with strict xylobiohydrolase function |
| topic | xylobiohydrolase glycoside hydrolase glycoside hydrolase family 30 AcXbh30A Acetivibrio clariflavus xylobiose |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2021.714238/full |
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