Synthesis of 1,4-dihydropyrimidines with immobilized urease: effect of method immobilization on magnetic supports
The effect of the urease immobilization method was studied on magnetic supports for the Biginelli/Hantzsch reaction. For this purpose, Fe3O4/SiO2 was modified with 3-aminopropyl-triethoxysilane and then activated with glutaraldehyde. A ratio of 500 mg of enzyme per gram of support at 4°C and 18 h we...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2017-08-01
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| Series: | Green Processing and Synthesis |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/gps-2016-0143 |
| _version_ | 1819291101396205568 |
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| author | Vargas Annie Y. Rojas Hugo A. Romanelli Gustavo P. Martínez José J. |
| author_facet | Vargas Annie Y. Rojas Hugo A. Romanelli Gustavo P. Martínez José J. |
| author_sort | Vargas Annie Y. |
| collection | DOAJ |
| description | The effect of the urease immobilization method was studied on magnetic supports for the Biginelli/Hantzsch reaction. For this purpose, Fe3O4/SiO2 was modified with 3-aminopropyl-triethoxysilane and then activated with glutaraldehyde. A ratio of 500 mg of enzyme per gram of support at 4°C and 18 h were sufficient for the physical adsorption, while 24 h were required for covalent bonding. The Biginelli and Hantzsch reactions were used to evaluate urease application in multicomponent reactions (MCRs). The synthesis of 1,4-dihydropyrimidines was successfully performed using immobilized urease favoring the Hantzsch product. The magnetic properties of the supports allow easy separation, and the urease immobilized by both methods improved the enzymatic activity compared to that of free urease. |
| first_indexed | 2024-12-24T03:33:17Z |
| format | Article |
| id | doaj.art-567b2afa5bb04b2d878b2d495d233079 |
| institution | Directory Open Access Journal |
| issn | 2191-9542 2191-9550 |
| language | English |
| last_indexed | 2024-12-24T03:33:17Z |
| publishDate | 2017-08-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Green Processing and Synthesis |
| spelling | doaj.art-567b2afa5bb04b2d878b2d495d2330792022-12-21T17:17:08ZengDe GruyterGreen Processing and Synthesis2191-95422191-95502017-08-016437738410.1515/gps-2016-0143Synthesis of 1,4-dihydropyrimidines with immobilized urease: effect of method immobilization on magnetic supportsVargas Annie Y.0Rojas Hugo A.1Romanelli Gustavo P.2Martínez José J.3Escuela de Ciencias Químicas, Facultad de Ciencias, Universidad Pedagógica y Tecnológica de Colombia UPTC, Avenida Central del Norte 39-115, Tunja, Boyacá, ColombiaEscuela de Ciencias Químicas, Facultad de Ciencias, Universidad Pedagógica y Tecnológica de Colombia UPTC, Avenida Central del Norte 39-115, Tunja, Boyacá, ColombiaCentro de Investigación y Desarrollo en Ciencias Aplicadas “Dr. J.J. Ronco” (CINDECA), Departamento de Química, Facultad de Ciencias Exactas, UNLP-CCT-CONICET. Calles 47 N° 257, B1900 AJK, La Plata, ArgentinaEscuela de Ciencias Químicas, Facultad de Ciencias, Universidad Pedagógica y Tecnológica de Colombia UPTC, Avenida Central del Norte 39-115, Tunja, Boyacá, ColombiaThe effect of the urease immobilization method was studied on magnetic supports for the Biginelli/Hantzsch reaction. For this purpose, Fe3O4/SiO2 was modified with 3-aminopropyl-triethoxysilane and then activated with glutaraldehyde. A ratio of 500 mg of enzyme per gram of support at 4°C and 18 h were sufficient for the physical adsorption, while 24 h were required for covalent bonding. The Biginelli and Hantzsch reactions were used to evaluate urease application in multicomponent reactions (MCRs). The synthesis of 1,4-dihydropyrimidines was successfully performed using immobilized urease favoring the Hantzsch product. The magnetic properties of the supports allow easy separation, and the urease immobilized by both methods improved the enzymatic activity compared to that of free urease.https://doi.org/10.1515/gps-2016-0143biginelli/hantzsch reactionmagnetic supportsurease immobilized |
| spellingShingle | Vargas Annie Y. Rojas Hugo A. Romanelli Gustavo P. Martínez José J. Synthesis of 1,4-dihydropyrimidines with immobilized urease: effect of method immobilization on magnetic supports Green Processing and Synthesis biginelli/hantzsch reaction magnetic supports urease immobilized |
| title | Synthesis of 1,4-dihydropyrimidines with immobilized urease: effect of method immobilization on magnetic supports |
| title_full | Synthesis of 1,4-dihydropyrimidines with immobilized urease: effect of method immobilization on magnetic supports |
| title_fullStr | Synthesis of 1,4-dihydropyrimidines with immobilized urease: effect of method immobilization on magnetic supports |
| title_full_unstemmed | Synthesis of 1,4-dihydropyrimidines with immobilized urease: effect of method immobilization on magnetic supports |
| title_short | Synthesis of 1,4-dihydropyrimidines with immobilized urease: effect of method immobilization on magnetic supports |
| title_sort | synthesis of 1 4 dihydropyrimidines with immobilized urease effect of method immobilization on magnetic supports |
| topic | biginelli/hantzsch reaction magnetic supports urease immobilized |
| url | https://doi.org/10.1515/gps-2016-0143 |
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