Genotyping of hexaploid wheat varieties from different Russian regions
We used molecular-genetic and molecular-cytology approaches to characterize the genomes of 20 varieties of wheat created in different regions of Russia. A molecular-genetic analysis was performed using 29 SSR-markers covering the entire genome, and 41 ISBP-markers localized on chromosome 5B. Analysi...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2016-03-01
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| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/523 |
| _version_ | 1797214142525865984 |
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| author | I. G. Adonina I. N. Leonova E. D. Badaeva E. A. Salina |
| author_facet | I. G. Adonina I. N. Leonova E. D. Badaeva E. A. Salina |
| author_sort | I. G. Adonina |
| collection | DOAJ |
| description | We used molecular-genetic and molecular-cytology approaches to characterize the genomes of 20 varieties of wheat created in different regions of Russia. A molecular-genetic analysis was performed using 29 SSR-markers covering the entire genome, and 41 ISBP-markers localized on chromosome 5B. Analysis of genetic similarity based on the results of molecular genotyping showed that the winter wheat varieties form a common cluster, regardless of the origin or area of cultivation. This is primarily due to the fact that the varieties originating from the European part of Russia were used to establish winter wheat varieties for West Siberia. Comparative analysis of individual dendrograms constructed using 1–2 markers per chromosome, and with the involvement of a larger number of 5B-chromosome markers allowed us to identify varieties with rearrangements of this chromosome and to assess genetic diversity. We found that winter wheat Vassa and spring wheat Chelyaba 75 were clustered closely together. This is an indirect confirmation of the use of winter wheat varieties in the breeding to improve the productive potential of spring wheat. Molecular-cytology analysis by C-banding and fluorescence in situ hybridization (FISH) revealed various chromosomal rearrangements in 8 of 20 cultivars studied, including translocations from S. cereale, Ae. speltoides and Th. intermedium. Thus, a combination of the two approaches allowed us to better characterize genomes of wheat varieties of various origin. |
| first_indexed | 2024-03-07T16:07:01Z |
| format | Article |
| id | doaj.art-567b3a0d1f0d48028d61ccb6e4c54a18 |
| institution | Directory Open Access Journal |
| issn | 2500-3259 |
| language | English |
| last_indexed | 2024-04-24T11:09:28Z |
| publishDate | 2016-03-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj.art-567b3a0d1f0d48028d61ccb6e4c54a182024-04-11T15:30:56ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592016-03-01201445010.18699/VJ16.107444Genotyping of hexaploid wheat varieties from different Russian regionsI. G. Adonina0I. N. Leonova1E. D. Badaeva2E. A. Salina3Institute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaVavilov Institute of General Genetics RAS, Moscow, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaWe used molecular-genetic and molecular-cytology approaches to characterize the genomes of 20 varieties of wheat created in different regions of Russia. A molecular-genetic analysis was performed using 29 SSR-markers covering the entire genome, and 41 ISBP-markers localized on chromosome 5B. Analysis of genetic similarity based on the results of molecular genotyping showed that the winter wheat varieties form a common cluster, regardless of the origin or area of cultivation. This is primarily due to the fact that the varieties originating from the European part of Russia were used to establish winter wheat varieties for West Siberia. Comparative analysis of individual dendrograms constructed using 1–2 markers per chromosome, and with the involvement of a larger number of 5B-chromosome markers allowed us to identify varieties with rearrangements of this chromosome and to assess genetic diversity. We found that winter wheat Vassa and spring wheat Chelyaba 75 were clustered closely together. This is an indirect confirmation of the use of winter wheat varieties in the breeding to improve the productive potential of spring wheat. Molecular-cytology analysis by C-banding and fluorescence in situ hybridization (FISH) revealed various chromosomal rearrangements in 8 of 20 cultivars studied, including translocations from S. cereale, Ae. speltoides and Th. intermedium. Thus, a combination of the two approaches allowed us to better characterize genomes of wheat varieties of various origin.https://vavilov.elpub.ru/jour/article/view/523triticum aestivumwinter and spring varietiesgenotypingssr- and isbp-markersc-bandingfluorescence in situ hybridization (fish). |
| spellingShingle | I. G. Adonina I. N. Leonova E. D. Badaeva E. A. Salina Genotyping of hexaploid wheat varieties from different Russian regions Вавиловский журнал генетики и селекции triticum aestivum winter and spring varieties genotyping ssr- and isbp-markers c-banding fluorescence in situ hybridization (fish). |
| title | Genotyping of hexaploid wheat varieties from different Russian regions |
| title_full | Genotyping of hexaploid wheat varieties from different Russian regions |
| title_fullStr | Genotyping of hexaploid wheat varieties from different Russian regions |
| title_full_unstemmed | Genotyping of hexaploid wheat varieties from different Russian regions |
| title_short | Genotyping of hexaploid wheat varieties from different Russian regions |
| title_sort | genotyping of hexaploid wheat varieties from different russian regions |
| topic | triticum aestivum winter and spring varieties genotyping ssr- and isbp-markers c-banding fluorescence in situ hybridization (fish). |
| url | https://vavilov.elpub.ru/jour/article/view/523 |
| work_keys_str_mv | AT igadonina genotypingofhexaploidwheatvarietiesfromdifferentrussianregions AT inleonova genotypingofhexaploidwheatvarietiesfromdifferentrussianregions AT edbadaeva genotypingofhexaploidwheatvarietiesfromdifferentrussianregions AT easalina genotypingofhexaploidwheatvarietiesfromdifferentrussianregions |