Anticancer Potential of Xanthohumol and Isoxanthohumol Loaded into SBA-15 Mesoporous Silica Particles against B16F10 Melanoma Cells
Xanthohumol (XN) and isoxanthohumol (IXN), prenylated flavonoids from <i>Humulus lupulus</i>, have been shown to possess antitumor/cancerprotective, antioxidant, antiinflammatory, and antiangiogenic properties. In this study, mesoporous silica (SBA-15) was loaded with different amounts o...
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| author | Tamara Krajnović Nebojša Đ. Pantelić Katharina Wolf Thomas Eichhorn Danijela Maksimović-Ivanić Sanja Mijatović Ludger A. Wessjohann Goran N. Kaluđerović |
| author_facet | Tamara Krajnović Nebojša Đ. Pantelić Katharina Wolf Thomas Eichhorn Danijela Maksimović-Ivanić Sanja Mijatović Ludger A. Wessjohann Goran N. Kaluđerović |
| author_sort | Tamara Krajnović |
| collection | DOAJ |
| description | Xanthohumol (XN) and isoxanthohumol (IXN), prenylated flavonoids from <i>Humulus lupulus</i>, have been shown to possess antitumor/cancerprotective, antioxidant, antiinflammatory, and antiangiogenic properties. In this study, mesoporous silica (SBA-15) was loaded with different amounts of xanthohumol and isoxanthohumol and characterized by standard analytical methods. The anticancer potential of XN and IXN loaded into SBA-15 has been evaluated against malignant mouse melanoma B16F10 cells. When these cells were treated with SBA-15 containing xanthohumol, an increase of the activity correlated with a higher immobilization rate of XN was observed. Considering the amount of XN loaded into SBA-15 (calculated from TGA), an improved antitumor potential of XN was observed (IC<sub>50</sub> = 10.8 ± 0.4 and 11.8 ± 0.5 µM for SBA-15|XN2 and SBA-15|XN3, respectively; vs. IC<sub>50</sub> = 18.5 ± 1.5 µM for free XN). The main mechanism against tumor cells of immobilized XN includes inhibition of proliferation and autophagic cell death. The MC<sub>50</sub> values for SBA-15 loaded with isoxanthohumol were over 300 µg/mL in all cases investigated. |
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| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T10:15:20Z |
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| spelling | doaj.art-567f0e891ae34447ad35f81ae671d7c22023-12-01T22:24:07ZengMDPI AGMaterials1996-19442022-07-011514502810.3390/ma15145028Anticancer Potential of Xanthohumol and Isoxanthohumol Loaded into SBA-15 Mesoporous Silica Particles against B16F10 Melanoma CellsTamara Krajnović0Nebojša Đ. Pantelić1Katharina Wolf2Thomas Eichhorn3Danijela Maksimović-Ivanić4Sanja Mijatović5Ludger A. Wessjohann6Goran N. Kaluđerović7Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Engineering and Natural Sciences, University of Applied Sciences Merseburg, Eberhard-Leibnitz-Straße 2, 06217 Merseburg, GermanyDepartment of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), GermanyDepartment of Engineering and Natural Sciences, University of Applied Sciences Merseburg, Eberhard-Leibnitz-Straße 2, 06217 Merseburg, GermanyInstitute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), GermanyDepartment of Engineering and Natural Sciences, University of Applied Sciences Merseburg, Eberhard-Leibnitz-Straße 2, 06217 Merseburg, GermanyXanthohumol (XN) and isoxanthohumol (IXN), prenylated flavonoids from <i>Humulus lupulus</i>, have been shown to possess antitumor/cancerprotective, antioxidant, antiinflammatory, and antiangiogenic properties. In this study, mesoporous silica (SBA-15) was loaded with different amounts of xanthohumol and isoxanthohumol and characterized by standard analytical methods. The anticancer potential of XN and IXN loaded into SBA-15 has been evaluated against malignant mouse melanoma B16F10 cells. When these cells were treated with SBA-15 containing xanthohumol, an increase of the activity correlated with a higher immobilization rate of XN was observed. Considering the amount of XN loaded into SBA-15 (calculated from TGA), an improved antitumor potential of XN was observed (IC<sub>50</sub> = 10.8 ± 0.4 and 11.8 ± 0.5 µM for SBA-15|XN2 and SBA-15|XN3, respectively; vs. IC<sub>50</sub> = 18.5 ± 1.5 µM for free XN). The main mechanism against tumor cells of immobilized XN includes inhibition of proliferation and autophagic cell death. The MC<sub>50</sub> values for SBA-15 loaded with isoxanthohumol were over 300 µg/mL in all cases investigated.https://www.mdpi.com/1996-1944/15/14/5028xanthohumolisoxanthohumolSBA-15cytotoxicitymelanoma |
| spellingShingle | Tamara Krajnović Nebojša Đ. Pantelić Katharina Wolf Thomas Eichhorn Danijela Maksimović-Ivanić Sanja Mijatović Ludger A. Wessjohann Goran N. Kaluđerović Anticancer Potential of Xanthohumol and Isoxanthohumol Loaded into SBA-15 Mesoporous Silica Particles against B16F10 Melanoma Cells Materials xanthohumol isoxanthohumol SBA-15 cytotoxicity melanoma |
| title | Anticancer Potential of Xanthohumol and Isoxanthohumol Loaded into SBA-15 Mesoporous Silica Particles against B16F10 Melanoma Cells |
| title_full | Anticancer Potential of Xanthohumol and Isoxanthohumol Loaded into SBA-15 Mesoporous Silica Particles against B16F10 Melanoma Cells |
| title_fullStr | Anticancer Potential of Xanthohumol and Isoxanthohumol Loaded into SBA-15 Mesoporous Silica Particles against B16F10 Melanoma Cells |
| title_full_unstemmed | Anticancer Potential of Xanthohumol and Isoxanthohumol Loaded into SBA-15 Mesoporous Silica Particles against B16F10 Melanoma Cells |
| title_short | Anticancer Potential of Xanthohumol and Isoxanthohumol Loaded into SBA-15 Mesoporous Silica Particles against B16F10 Melanoma Cells |
| title_sort | anticancer potential of xanthohumol and isoxanthohumol loaded into sba 15 mesoporous silica particles against b16f10 melanoma cells |
| topic | xanthohumol isoxanthohumol SBA-15 cytotoxicity melanoma |
| url | https://www.mdpi.com/1996-1944/15/14/5028 |
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