Optimisation of the two-dimensional gel electrophoresis protocol using the Taguchi approach

Optimisation of the two-dimensional gel electrophoresis protocol using the Taguchi approach

<p>Abstract</p> <p>Background</p> <p>Quantitative proteomic analyses have traditionally used two-dimensional gel electrophoresis (2DE) for separation and characterisation of complex protein mixtures. Among the difficulties associated with this approach is the solubilisa...

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Bibliographic Details
Main Authors: Blow J Julian, Porter Iain M, Khoudoli Guennadi A, Swedlow Jason R
Format: Article
Language:English
Published: BMC 2004-09-01
Series:Proteome Science
Subjects:
<it>Xenopus </it>egg extract
chromatin
cell cycle
two-dimensional gel electrophoresis
proteomics
Taguchi method
optimisation
Online Access:http://www.proteomesci.com/content/2/1/6

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