Abundance and diversity of Streptomycescommunities on six land covertypes of the coffee belt in Quindío, Colombia

Community structure of the Streptomycesgenus was compare for six land cover types ofthe coffee belt in Quindío (Central Cordillera, Colombian Andes). A plate counting method was used to estimate abundance and morphotypic diversity. Genetic diversity was studied by PCR-RFLP of ribosomal 16S genes. Li...

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Main Authors: Pilar Corredor, Eugenio Andrade, Joseph Tohme, Myriam Duque, Camilo Flórez
Format: Article
Language:English
Published: Universidad Nacional de Colombia 2000-01-01
Series:Acta Biológica Colombiana
Subjects:
Online Access:https://revistas.unal.edu.co/index.php/actabiol/article/view/26681
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author Pilar Corredor
Eugenio Andrade
Joseph Tohme
Myriam Duque
Camilo Flórez
author_facet Pilar Corredor
Eugenio Andrade
Joseph Tohme
Myriam Duque
Camilo Flórez
author_sort Pilar Corredor
collection DOAJ
description Community structure of the Streptomycesgenus was compare for six land cover types ofthe coffee belt in Quindío (Central Cordillera, Colombian Andes). A plate counting method was used to estimate abundance and morphotypic diversity. Genetic diversity was studied by PCR-RFLP of ribosomal 16S genes. Likewise, ribosomal restriction analysis was used to determine genetic diversity from soil sample DNA community, including the non-cultivable fraction of the community. The analysis showed significant statistical differences on the abundance of Streptomycesamong the six cover types. Similar values of morphotypic diversity were obtained for the six cover types, having “guaduales” and pasture the highest values. It was found that genetic and morphological diversity were dissimilar, except for coffee shaded by banana and Inga. In the culture-independent analysis each cover type was characterized by specificRFLP pattern, the forest cover type presented the highest diversity. The results show that modifications in the cover type exert an influence on Streptomyces soil communities resulting in abundance and diversity variations.
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spelling doaj.art-5688f4d7bbb64f1db759efee65e4b14e2022-12-21T16:58:20ZengUniversidad Nacional de ColombiaActa Biológica Colombiana0120-548X1900-16492000-01-0151596923723Abundance and diversity of Streptomycescommunities on six land covertypes of the coffee belt in Quindío, ColombiaPilar Corredor0Eugenio Andrade1Joseph Tohme2Myriam Duque3Camilo Flórez4Universidad Nacional de ColombiaUniversidad Nacional de ColombiaInstituto Alexander von HumboldtInstituto Alexander von HumboldtDivisión de BiotecnologíaCommunity structure of the Streptomycesgenus was compare for six land cover types ofthe coffee belt in Quindío (Central Cordillera, Colombian Andes). A plate counting method was used to estimate abundance and morphotypic diversity. Genetic diversity was studied by PCR-RFLP of ribosomal 16S genes. Likewise, ribosomal restriction analysis was used to determine genetic diversity from soil sample DNA community, including the non-cultivable fraction of the community. The analysis showed significant statistical differences on the abundance of Streptomycesamong the six cover types. Similar values of morphotypic diversity were obtained for the six cover types, having “guaduales” and pasture the highest values. It was found that genetic and morphological diversity were dissimilar, except for coffee shaded by banana and Inga. In the culture-independent analysis each cover type was characterized by specificRFLP pattern, the forest cover type presented the highest diversity. The results show that modifications in the cover type exert an influence on Streptomyces soil communities resulting in abundance and diversity variations.https://revistas.unal.edu.co/index.php/actabiol/article/view/26681Ecología de StreptomycesPcr-RflpCultivo en Placa
spellingShingle Pilar Corredor
Eugenio Andrade
Joseph Tohme
Myriam Duque
Camilo Flórez
Abundance and diversity of Streptomycescommunities on six land covertypes of the coffee belt in Quindío, Colombia
Acta Biológica Colombiana
Ecología de Streptomyces
Pcr-Rflp
Cultivo en Placa
title Abundance and diversity of Streptomycescommunities on six land covertypes of the coffee belt in Quindío, Colombia
title_full Abundance and diversity of Streptomycescommunities on six land covertypes of the coffee belt in Quindío, Colombia
title_fullStr Abundance and diversity of Streptomycescommunities on six land covertypes of the coffee belt in Quindío, Colombia
title_full_unstemmed Abundance and diversity of Streptomycescommunities on six land covertypes of the coffee belt in Quindío, Colombia
title_short Abundance and diversity of Streptomycescommunities on six land covertypes of the coffee belt in Quindío, Colombia
title_sort abundance and diversity of streptomycescommunities on six land covertypes of the coffee belt in quindio colombia
topic Ecología de Streptomyces
Pcr-Rflp
Cultivo en Placa
url https://revistas.unal.edu.co/index.php/actabiol/article/view/26681
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