Chemical Composition, Antioxidant Properties, and Antibacterial Activity of Essential Oils of <i>Satureja macrostema</i> (Moc. and Sessé ex Benth.) Briq
<i>Satureja macrostema</i> is a plant that is located in various regions of Mexico and is used in a traditional way against illness. Essential oils (EOs) were obtained from leaves <i>Satureja macrostema</i> and the chemical composition was evaluated by gas chromatography–mass...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/12/4719 |
| _version_ | 1797593311063572480 |
|---|---|
| author | Lucia Barrientos Ramírez José Antonio Silva Guzmán Edison Antonio Osorio Muñoz Carlos Alvarez Moya Mónica Reynoso Silva Abraham Francisco Cetina Corona Josefina Casas Solis J. Jesús Vargas Radillo |
| author_facet | Lucia Barrientos Ramírez José Antonio Silva Guzmán Edison Antonio Osorio Muñoz Carlos Alvarez Moya Mónica Reynoso Silva Abraham Francisco Cetina Corona Josefina Casas Solis J. Jesús Vargas Radillo |
| author_sort | Lucia Barrientos Ramírez |
| collection | DOAJ |
| description | <i>Satureja macrostema</i> is a plant that is located in various regions of Mexico and is used in a traditional way against illness. Essential oils (EOs) were obtained from leaves <i>Satureja macrostema</i> and the chemical composition was evaluated by gas chromatography–mass spectrometry (GC-MS). The antioxidant effect of the oil was assayed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and by Trolox Equivalent Antioxidant Capacity (TEAC). In vitro antibacterial activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> was determined using a broth microdilution assay and thin layer chromatography–direct bioautography (TLC-DB) to identify active antibacterial compounds. The EOs analysis showed 21 compounds, 99% terpenes, and 96% oxygenated monoterpenes, with <i>trans</i>-piperitone epoxide (46%), <i>cis</i>-piperitone epoxide (22%), and piperitenone oxide (11%) as more abundant compounds. Likewise, <i>S. macrostema</i> EOs showed an antioxidant activity of DPPH = 82%, with 50% free radical scavenging (IC<sub>50</sub>) = 7 mg/mL and TEAC = 0.005, an antibacterial effect against <i>E. coli</i> of 73% inhibition, and 81% over <i>S. aureus</i> at dose of 100 µL of undiluted crude oil. The TLC-DB assay showed that the most active compounds were derived from piperitone. The comparison with other studies on <i>S. macrostema</i> shows variability in the compounds and their abundances, which can be attributed to climatic factors and the maturity of plants with similar antioxidant and antibacterial activities. |
| first_indexed | 2024-03-11T02:06:14Z |
| format | Article |
| id | doaj.art-568bfeec3c364283aaa0fad071a61ea1 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T02:06:14Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-568bfeec3c364283aaa0fad071a61ea12023-11-18T11:49:25ZengMDPI AGMolecules1420-30492023-06-012812471910.3390/molecules28124719Chemical Composition, Antioxidant Properties, and Antibacterial Activity of Essential Oils of <i>Satureja macrostema</i> (Moc. and Sessé ex Benth.) BriqLucia Barrientos Ramírez0José Antonio Silva Guzmán1Edison Antonio Osorio Muñoz2Carlos Alvarez Moya3Mónica Reynoso Silva4Abraham Francisco Cetina Corona5Josefina Casas Solis6J. Jesús Vargas Radillo7Department of Wood, Cellulose and Paper, University of Guadalajara, CUCEI, Road Ing. Ramón Padilla Sánchez 2100, Las Agujas, Zapopan 45200, Jalisco, MexicoDepartment of Wood, Cellulose and Paper, University of Guadalajara, CUCEI, Road Ing. Ramón Padilla Sánchez 2100, Las Agujas, Zapopan 45200, Jalisco, MexicoGroup of Research and Development in Science Applied to Biological Resources, Salesian Polytechnic University, 12 de Octubre Avenue N2422 and Wilson, Quito 170109, EcuadorEnvironmental Mutagenesis Laboratory, Cellular and Molecular Department, University of Guadalajara, Guadalajara 45200, Jalisco, MexicoEnvironmental Mutagenesis Laboratory, Cellular and Molecular Department, University of Guadalajara, Guadalajara 45200, Jalisco, MexicoDepartment of Wood, Cellulose and Paper, University of Guadalajara, CUCEI, Road Ing. Ramón Padilla Sánchez 2100, Las Agujas, Zapopan 45200, Jalisco, MexicoMicrobiology Laboratory, Cellular and Molecular Department, University Center for Biological and Agricultural Sciences (CUCBA), Road Ing. Ramón Padilla Sánchez 2100, Nextipac, Zapopan 45200, Jalisco, MexicoDepartment of Wood, Cellulose and Paper, University of Guadalajara, CUCEI, Road Ing. Ramón Padilla Sánchez 2100, Las Agujas, Zapopan 45200, Jalisco, Mexico<i>Satureja macrostema</i> is a plant that is located in various regions of Mexico and is used in a traditional way against illness. Essential oils (EOs) were obtained from leaves <i>Satureja macrostema</i> and the chemical composition was evaluated by gas chromatography–mass spectrometry (GC-MS). The antioxidant effect of the oil was assayed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and by Trolox Equivalent Antioxidant Capacity (TEAC). In vitro antibacterial activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> was determined using a broth microdilution assay and thin layer chromatography–direct bioautography (TLC-DB) to identify active antibacterial compounds. The EOs analysis showed 21 compounds, 99% terpenes, and 96% oxygenated monoterpenes, with <i>trans</i>-piperitone epoxide (46%), <i>cis</i>-piperitone epoxide (22%), and piperitenone oxide (11%) as more abundant compounds. Likewise, <i>S. macrostema</i> EOs showed an antioxidant activity of DPPH = 82%, with 50% free radical scavenging (IC<sub>50</sub>) = 7 mg/mL and TEAC = 0.005, an antibacterial effect against <i>E. coli</i> of 73% inhibition, and 81% over <i>S. aureus</i> at dose of 100 µL of undiluted crude oil. The TLC-DB assay showed that the most active compounds were derived from piperitone. The comparison with other studies on <i>S. macrostema</i> shows variability in the compounds and their abundances, which can be attributed to climatic factors and the maturity of plants with similar antioxidant and antibacterial activities.https://www.mdpi.com/1420-3049/28/12/4719piperitone derivativesdirect bioautographyTEACLamiaceaechemotypes |
| spellingShingle | Lucia Barrientos Ramírez José Antonio Silva Guzmán Edison Antonio Osorio Muñoz Carlos Alvarez Moya Mónica Reynoso Silva Abraham Francisco Cetina Corona Josefina Casas Solis J. Jesús Vargas Radillo Chemical Composition, Antioxidant Properties, and Antibacterial Activity of Essential Oils of <i>Satureja macrostema</i> (Moc. and Sessé ex Benth.) Briq Molecules piperitone derivatives direct bioautography TEAC Lamiaceae chemotypes |
| title | Chemical Composition, Antioxidant Properties, and Antibacterial Activity of Essential Oils of <i>Satureja macrostema</i> (Moc. and Sessé ex Benth.) Briq |
| title_full | Chemical Composition, Antioxidant Properties, and Antibacterial Activity of Essential Oils of <i>Satureja macrostema</i> (Moc. and Sessé ex Benth.) Briq |
| title_fullStr | Chemical Composition, Antioxidant Properties, and Antibacterial Activity of Essential Oils of <i>Satureja macrostema</i> (Moc. and Sessé ex Benth.) Briq |
| title_full_unstemmed | Chemical Composition, Antioxidant Properties, and Antibacterial Activity of Essential Oils of <i>Satureja macrostema</i> (Moc. and Sessé ex Benth.) Briq |
| title_short | Chemical Composition, Antioxidant Properties, and Antibacterial Activity of Essential Oils of <i>Satureja macrostema</i> (Moc. and Sessé ex Benth.) Briq |
| title_sort | chemical composition antioxidant properties and antibacterial activity of essential oils of i satureja macrostema i moc and sesse ex benth briq |
| topic | piperitone derivatives direct bioautography TEAC Lamiaceae chemotypes |
| url | https://www.mdpi.com/1420-3049/28/12/4719 |
| work_keys_str_mv | AT luciabarrientosramirez chemicalcompositionantioxidantpropertiesandantibacterialactivityofessentialoilsofisaturejamacrostemaimocandsesseexbenthbriq AT joseantoniosilvaguzman chemicalcompositionantioxidantpropertiesandantibacterialactivityofessentialoilsofisaturejamacrostemaimocandsesseexbenthbriq AT edisonantonioosoriomunoz chemicalcompositionantioxidantpropertiesandantibacterialactivityofessentialoilsofisaturejamacrostemaimocandsesseexbenthbriq AT carlosalvarezmoya chemicalcompositionantioxidantpropertiesandantibacterialactivityofessentialoilsofisaturejamacrostemaimocandsesseexbenthbriq AT monicareynososilva chemicalcompositionantioxidantpropertiesandantibacterialactivityofessentialoilsofisaturejamacrostemaimocandsesseexbenthbriq AT abrahamfranciscocetinacorona chemicalcompositionantioxidantpropertiesandantibacterialactivityofessentialoilsofisaturejamacrostemaimocandsesseexbenthbriq AT josefinacasassolis chemicalcompositionantioxidantpropertiesandantibacterialactivityofessentialoilsofisaturejamacrostemaimocandsesseexbenthbriq AT jjesusvargasradillo chemicalcompositionantioxidantpropertiesandantibacterialactivityofessentialoilsofisaturejamacrostemaimocandsesseexbenthbriq |