Sulfation of sialic acid is ubiquitous and essential for vertebrate development
Abstract Glycosylation of proteins and lipids occurs in vertebrates, usually terminating with sialylation, which regulates the physicochemical and biological properties of these glycoconjugates. Although less commonly known, sialic acid residues also undergo various modifications, such as acetylatio...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-15143-4 |
| _version_ | 1811287113285500928 |
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| author | Nursah Ertunc Thanyaluck Phitak Di Wu Hiroshi Fujita Masaya Hane Chihiro Sato Ken Kitajima |
| author_facet | Nursah Ertunc Thanyaluck Phitak Di Wu Hiroshi Fujita Masaya Hane Chihiro Sato Ken Kitajima |
| author_sort | Nursah Ertunc |
| collection | DOAJ |
| description | Abstract Glycosylation of proteins and lipids occurs in vertebrates, usually terminating with sialylation, which regulates the physicochemical and biological properties of these glycoconjugates. Although less commonly known, sialic acid residues also undergo various modifications, such as acetylation, methylation, and sulfation. However, except for acetylation, the enzymes or functions of the other modification processes are unknown. To the best of our knowledge, this study is the first to demonstrate the ubiquitous occurrence of sulfated sialic acids and two genes encoding the sialate: O-sulfotransferases 1 and 2 in vertebrates. These two enzymes showed about 50% amino acid sequence identity, and appeared to be complementary to each other in acceptor substrate preferences. Gene targeting experiments showed that the deficiency of these genes was lethal for medaka fish during young fry development and accompanied by different phenotypes. Thus, the sulfation of sialic acids is essential for the vertebrate development. |
| first_indexed | 2024-04-13T03:13:10Z |
| format | Article |
| id | doaj.art-568c8516a37244f88499dd4353b59154 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T03:13:10Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-568c8516a37244f88499dd4353b591542022-12-22T03:04:59ZengNature PortfolioScientific Reports2045-23222022-07-0112111410.1038/s41598-022-15143-4Sulfation of sialic acid is ubiquitous and essential for vertebrate developmentNursah Ertunc0Thanyaluck Phitak1Di Wu2Hiroshi Fujita3Masaya Hane4Chihiro Sato5Ken Kitajima6Bioscience and Biotechnology Center, and Graduate School of Bioagricultural Sciences, Nagoya UniversityBioscience and Biotechnology Center, and Graduate School of Bioagricultural Sciences, Nagoya UniversityBioscience and Biotechnology Center, and Graduate School of Bioagricultural Sciences, Nagoya UniversityBioscience and Biotechnology Center, and Graduate School of Bioagricultural Sciences, Nagoya UniversityBioscience and Biotechnology Center, and Graduate School of Bioagricultural Sciences, Nagoya UniversityBioscience and Biotechnology Center, and Graduate School of Bioagricultural Sciences, Nagoya UniversityBioscience and Biotechnology Center, and Graduate School of Bioagricultural Sciences, Nagoya UniversityAbstract Glycosylation of proteins and lipids occurs in vertebrates, usually terminating with sialylation, which regulates the physicochemical and biological properties of these glycoconjugates. Although less commonly known, sialic acid residues also undergo various modifications, such as acetylation, methylation, and sulfation. However, except for acetylation, the enzymes or functions of the other modification processes are unknown. To the best of our knowledge, this study is the first to demonstrate the ubiquitous occurrence of sulfated sialic acids and two genes encoding the sialate: O-sulfotransferases 1 and 2 in vertebrates. These two enzymes showed about 50% amino acid sequence identity, and appeared to be complementary to each other in acceptor substrate preferences. Gene targeting experiments showed that the deficiency of these genes was lethal for medaka fish during young fry development and accompanied by different phenotypes. Thus, the sulfation of sialic acids is essential for the vertebrate development.https://doi.org/10.1038/s41598-022-15143-4 |
| spellingShingle | Nursah Ertunc Thanyaluck Phitak Di Wu Hiroshi Fujita Masaya Hane Chihiro Sato Ken Kitajima Sulfation of sialic acid is ubiquitous and essential for vertebrate development Scientific Reports |
| title | Sulfation of sialic acid is ubiquitous and essential for vertebrate development |
| title_full | Sulfation of sialic acid is ubiquitous and essential for vertebrate development |
| title_fullStr | Sulfation of sialic acid is ubiquitous and essential for vertebrate development |
| title_full_unstemmed | Sulfation of sialic acid is ubiquitous and essential for vertebrate development |
| title_short | Sulfation of sialic acid is ubiquitous and essential for vertebrate development |
| title_sort | sulfation of sialic acid is ubiquitous and essential for vertebrate development |
| url | https://doi.org/10.1038/s41598-022-15143-4 |
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