Temporal variation in oral microbiome composition of patients undergoing autologous hematopoietic cell transplantation with keratinocyte growth factor
Abstract Introduction Autologous hematopoietic cell transplantation (AHCT) is a well-established treatment for lymphoma. Unintended effects of this therapy include oral mucositis (OM) and gastrointestinal toxicities, resulting in poor clinical outcomes. The gut microbiome has been previously linked...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-03000-x |
| _version_ | 1827724257738620928 |
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| author | Bruno Bohn Miroslava Chalupova Christopher Staley Shernan Holtan Joseph Maakaron Veronika Bachanova Najla El Jurdi |
| author_facet | Bruno Bohn Miroslava Chalupova Christopher Staley Shernan Holtan Joseph Maakaron Veronika Bachanova Najla El Jurdi |
| author_sort | Bruno Bohn |
| collection | DOAJ |
| description | Abstract Introduction Autologous hematopoietic cell transplantation (AHCT) is a well-established treatment for lymphoma. Unintended effects of this therapy include oral mucositis (OM) and gastrointestinal toxicities, resulting in poor clinical outcomes. The gut microbiome has been previously linked to transplant toxicities among allogeneic recipients, but little is known about the effects of AHCT on the oral microbiome. Methods Seven patients with non-Hodgkin or Hodgkin lymphoma undergoing AHCT with palifermin (keratinocyte growth factor) were included. Buccal swab samples were collected at baseline and 14- and 28-days post-treatment. Oral microbial communities were characterized with 16 S rRNA amplicon sequencing. Temporal trends in community composition, alpha diversity, and beta diversity were investigated. Results A significant reduction in the relative abundance of the genera Gemella and Actinomyces were observed from baseline. No significant temporal differences in alpha diversity were observed. Significant changes in beta diversity were recorded. Conclusion Results of this pilot study suggest treatment with AHCT and palifermin affects the oral microbiome, resulting in temporal shifts in oral microbial community composition. Future studies are warranted to confirm these trends and further investigate the effects of AHCT on the oral microbiome and how these shifts may affect health outcomes. |
| first_indexed | 2024-03-10T22:13:15Z |
| format | Article |
| id | doaj.art-568f4ed1c7304cf1811ad5c92e9c5984 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-10T22:13:15Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-568f4ed1c7304cf1811ad5c92e9c59842023-11-19T12:32:52ZengBMCBMC Microbiology1471-21802023-09-012311710.1186/s12866-023-03000-xTemporal variation in oral microbiome composition of patients undergoing autologous hematopoietic cell transplantation with keratinocyte growth factorBruno Bohn0Miroslava Chalupova1Christopher Staley2Shernan Holtan3Joseph Maakaron4Veronika Bachanova5Najla El Jurdi6Division of Epidemiology & Community Health, School of Public Health, University of MinnesotaDepartment of Stomatology, Faculty of Medicine and University Hospital in Pilsen, Charles UniversityDepartment of Surgery, Medical School, University of MinnesotaDivision of Hematology, Oncology, and Transplantation, Department of Medicine, University of MinnesotaDivision of Hematology, Oncology, and Transplantation, Department of Medicine, University of MinnesotaDivision of Hematology, Oncology, and Transplantation, Department of Medicine, University of MinnesotaDivision of Hematology, Oncology, and Transplantation, Department of Medicine, University of MinnesotaAbstract Introduction Autologous hematopoietic cell transplantation (AHCT) is a well-established treatment for lymphoma. Unintended effects of this therapy include oral mucositis (OM) and gastrointestinal toxicities, resulting in poor clinical outcomes. The gut microbiome has been previously linked to transplant toxicities among allogeneic recipients, but little is known about the effects of AHCT on the oral microbiome. Methods Seven patients with non-Hodgkin or Hodgkin lymphoma undergoing AHCT with palifermin (keratinocyte growth factor) were included. Buccal swab samples were collected at baseline and 14- and 28-days post-treatment. Oral microbial communities were characterized with 16 S rRNA amplicon sequencing. Temporal trends in community composition, alpha diversity, and beta diversity were investigated. Results A significant reduction in the relative abundance of the genera Gemella and Actinomyces were observed from baseline. No significant temporal differences in alpha diversity were observed. Significant changes in beta diversity were recorded. Conclusion Results of this pilot study suggest treatment with AHCT and palifermin affects the oral microbiome, resulting in temporal shifts in oral microbial community composition. Future studies are warranted to confirm these trends and further investigate the effects of AHCT on the oral microbiome and how these shifts may affect health outcomes.https://doi.org/10.1186/s12866-023-03000-xHematopoietic cell transplantationOral microbiomeAutologous |
| spellingShingle | Bruno Bohn Miroslava Chalupova Christopher Staley Shernan Holtan Joseph Maakaron Veronika Bachanova Najla El Jurdi Temporal variation in oral microbiome composition of patients undergoing autologous hematopoietic cell transplantation with keratinocyte growth factor BMC Microbiology Hematopoietic cell transplantation Oral microbiome Autologous |
| title | Temporal variation in oral microbiome composition of patients undergoing autologous hematopoietic cell transplantation with keratinocyte growth factor |
| title_full | Temporal variation in oral microbiome composition of patients undergoing autologous hematopoietic cell transplantation with keratinocyte growth factor |
| title_fullStr | Temporal variation in oral microbiome composition of patients undergoing autologous hematopoietic cell transplantation with keratinocyte growth factor |
| title_full_unstemmed | Temporal variation in oral microbiome composition of patients undergoing autologous hematopoietic cell transplantation with keratinocyte growth factor |
| title_short | Temporal variation in oral microbiome composition of patients undergoing autologous hematopoietic cell transplantation with keratinocyte growth factor |
| title_sort | temporal variation in oral microbiome composition of patients undergoing autologous hematopoietic cell transplantation with keratinocyte growth factor |
| topic | Hematopoietic cell transplantation Oral microbiome Autologous |
| url | https://doi.org/10.1186/s12866-023-03000-x |
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