Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution
Silk from Bombyx mori has two main proteins: fibroin and sericin. Fibroin is a protein that exhibits good biocompatibility and high surface reactivity, desirable properties for many biomedical applications. Sericin is related with adverse immune response in some medical uses. Therefore, its removal...
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| Format: | Article |
| Language: | English |
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Universidad de Antioquia
2020-10-01
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| Series: | Revista Facultad de Ingeniería Universidad de Antioquia |
| Subjects: | |
| Online Access: | https://revistas.udea.edu.co/index.php/ingenieria/article/view/336655 |
| _version_ | 1797861820768190464 |
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| author | Estefanía Echeverri Correa David Orlando Grajales Lopera Santiago Gutiérrez Restrepo Claudia Patricia Ossa-Orozco |
| author_facet | Estefanía Echeverri Correa David Orlando Grajales Lopera Santiago Gutiérrez Restrepo Claudia Patricia Ossa-Orozco |
| author_sort | Estefanía Echeverri Correa |
| collection | DOAJ |
| description |
Silk from Bombyx mori has two main proteins: fibroin and sericin. Fibroin is a protein that exhibits good biocompatibility and high surface reactivity, desirable properties for many biomedical applications. Sericin is related with adverse immune response in some medical uses. Therefore, its removal (degumming) is desirable in almost all fields, including the textile industry and biological applications. In this study, three degumming methods (distilled water, liquid neutral detergent and sodium carbonate solution) were evaluated using Raman spectroscopy. Degummed silk treated with sodium carbonate solution exhibits a pattern consistent with previous studies for sericin-free fibroin, and significant differences with the untreated silk pattern. Also, degummed fibroin fibers were dissolved in a calcium chloride solution, in pursuit of a more versatile material. Additionally, different porous membranes of dialysis tubes were tested to remove residual salts, and were compared by conductivity measurements and EDS analysis, identifying good performance for an affordable food cellulose membrane. The results showed that it is possible to obtain fibroin with adequate chemical properties, using low-cost process and membrane of dialysis tubes.
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| first_indexed | 2024-04-09T22:09:24Z |
| format | Article |
| id | doaj.art-5690ce36e88044a5a376f0bfa945dd74 |
| institution | Directory Open Access Journal |
| issn | 0120-6230 2422-2844 |
| language | English |
| last_indexed | 2024-04-09T22:09:24Z |
| publishDate | 2020-10-01 |
| publisher | Universidad de Antioquia |
| record_format | Article |
| series | Revista Facultad de Ingeniería Universidad de Antioquia |
| spelling | doaj.art-5690ce36e88044a5a376f0bfa945dd742023-03-23T12:28:03ZengUniversidad de AntioquiaRevista Facultad de Ingeniería Universidad de Antioquia0120-62302422-28442020-10-019410.17533/udea.redin.20190731336655Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solutionEstefanía Echeverri Correa0David Orlando Grajales Lopera1Santiago Gutiérrez Restrepo2Claudia Patricia Ossa-Orozco3University of AntioquiaUniversity of AntioquiaUniversity of AntioquiaUniversity of Antioquia Silk from Bombyx mori has two main proteins: fibroin and sericin. Fibroin is a protein that exhibits good biocompatibility and high surface reactivity, desirable properties for many biomedical applications. Sericin is related with adverse immune response in some medical uses. Therefore, its removal (degumming) is desirable in almost all fields, including the textile industry and biological applications. In this study, three degumming methods (distilled water, liquid neutral detergent and sodium carbonate solution) were evaluated using Raman spectroscopy. Degummed silk treated with sodium carbonate solution exhibits a pattern consistent with previous studies for sericin-free fibroin, and significant differences with the untreated silk pattern. Also, degummed fibroin fibers were dissolved in a calcium chloride solution, in pursuit of a more versatile material. Additionally, different porous membranes of dialysis tubes were tested to remove residual salts, and were compared by conductivity measurements and EDS analysis, identifying good performance for an affordable food cellulose membrane. The results showed that it is possible to obtain fibroin with adequate chemical properties, using low-cost process and membrane of dialysis tubes. https://revistas.udea.edu.co/index.php/ingenieria/article/view/336655silk fibroin dialysisdegumming processmembranetissue engineering |
| spellingShingle | Estefanía Echeverri Correa David Orlando Grajales Lopera Santiago Gutiérrez Restrepo Claudia Patricia Ossa-Orozco Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution Revista Facultad de Ingeniería Universidad de Antioquia silk fibroin dialysis degumming process membrane tissue engineering |
| title | Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution |
| title_full | Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution |
| title_fullStr | Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution |
| title_full_unstemmed | Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution |
| title_short | Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution |
| title_sort | effective sericin fibroin separation from bombyx mori silkworms fibers and low cost salt removal from fibroin solution |
| topic | silk fibroin dialysis degumming process membrane tissue engineering |
| url | https://revistas.udea.edu.co/index.php/ingenieria/article/view/336655 |
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