Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus

Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus

We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay prod...

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Bibliographic Details
Main Authors: Shanthi Jayawardena, Chung Y. Cheung, Ian Barr, Kwok H. Chan, Honglin Chen, Yi Guan, J.S. Malik Peiris, Leo L.M. Poon
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2007-06-01
Series:Emerging Infectious Diseases
Subjects:
H5N1
avian influenza virus
molecular detection
loop-mediated isothermal amplification
dispatch
Online Access:https://wwwnc.cdc.gov/eid/article/13/6/06-1572_article
Description
Summary:We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.
ISSN:1080-6040
1080-6059

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