Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus

We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay prod...

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Main Authors: Shanthi Jayawardena, Chung Y. Cheung, Ian Barr, Kwok H. Chan, Honglin Chen, Yi Guan, J.S. Malik Peiris, Leo L.M. Poon
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2007-06-01
Series:Emerging Infectious Diseases
Subjects:
Online Access:https://wwwnc.cdc.gov/eid/article/13/6/06-1572_article
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author Shanthi Jayawardena
Chung Y. Cheung
Ian Barr
Kwok H. Chan
Honglin Chen
Yi Guan
J.S. Malik Peiris
Leo L.M. Poon
author_facet Shanthi Jayawardena
Chung Y. Cheung
Ian Barr
Kwok H. Chan
Honglin Chen
Yi Guan
J.S. Malik Peiris
Leo L.M. Poon
author_sort Shanthi Jayawardena
collection DOAJ
description We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.
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spelling doaj.art-5690eff7c61340598a6123a848784c352022-12-22T03:36:52ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592007-06-0113689990110.3201/eid1306.061572Loop-Mediated Isothermal Amplification for Influenza A (H5N1) VirusShanthi JayawardenaChung Y. CheungIan BarrKwok H. ChanHonglin ChenYi GuanJ.S. Malik PeirisLeo L.M. PoonWe describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.https://wwwnc.cdc.gov/eid/article/13/6/06-1572_articleH5N1avian influenza virusmolecular detectionloop-mediated isothermal amplificationdispatch
spellingShingle Shanthi Jayawardena
Chung Y. Cheung
Ian Barr
Kwok H. Chan
Honglin Chen
Yi Guan
J.S. Malik Peiris
Leo L.M. Poon
Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus
Emerging Infectious Diseases
H5N1
avian influenza virus
molecular detection
loop-mediated isothermal amplification
dispatch
title Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus
title_full Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus
title_fullStr Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus
title_full_unstemmed Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus
title_short Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus
title_sort loop mediated isothermal amplification for influenza a h5n1 virus
topic H5N1
avian influenza virus
molecular detection
loop-mediated isothermal amplification
dispatch
url https://wwwnc.cdc.gov/eid/article/13/6/06-1572_article
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