Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus
We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay prod...
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
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Centers for Disease Control and Prevention
2007-06-01
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Series: | Emerging Infectious Diseases |
Subjects: | |
Online Access: | https://wwwnc.cdc.gov/eid/article/13/6/06-1572_article |
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author | Shanthi Jayawardena Chung Y. Cheung Ian Barr Kwok H. Chan Honglin Chen Yi Guan J.S. Malik Peiris Leo L.M. Poon |
author_facet | Shanthi Jayawardena Chung Y. Cheung Ian Barr Kwok H. Chan Honglin Chen Yi Guan J.S. Malik Peiris Leo L.M. Poon |
author_sort | Shanthi Jayawardena |
collection | DOAJ |
description | We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes. |
first_indexed | 2024-04-12T10:30:19Z |
format | Article |
id | doaj.art-5690eff7c61340598a6123a848784c35 |
institution | Directory Open Access Journal |
issn | 1080-6040 1080-6059 |
language | English |
last_indexed | 2024-04-12T10:30:19Z |
publishDate | 2007-06-01 |
publisher | Centers for Disease Control and Prevention |
record_format | Article |
series | Emerging Infectious Diseases |
spelling | doaj.art-5690eff7c61340598a6123a848784c352022-12-22T03:36:52ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592007-06-0113689990110.3201/eid1306.061572Loop-Mediated Isothermal Amplification for Influenza A (H5N1) VirusShanthi JayawardenaChung Y. CheungIan BarrKwok H. ChanHonglin ChenYi GuanJ.S. Malik PeirisLeo L.M. PoonWe describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.https://wwwnc.cdc.gov/eid/article/13/6/06-1572_articleH5N1avian influenza virusmolecular detectionloop-mediated isothermal amplificationdispatch |
spellingShingle | Shanthi Jayawardena Chung Y. Cheung Ian Barr Kwok H. Chan Honglin Chen Yi Guan J.S. Malik Peiris Leo L.M. Poon Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus Emerging Infectious Diseases H5N1 avian influenza virus molecular detection loop-mediated isothermal amplification dispatch |
title | Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus |
title_full | Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus |
title_fullStr | Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus |
title_full_unstemmed | Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus |
title_short | Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus |
title_sort | loop mediated isothermal amplification for influenza a h5n1 virus |
topic | H5N1 avian influenza virus molecular detection loop-mediated isothermal amplification dispatch |
url | https://wwwnc.cdc.gov/eid/article/13/6/06-1572_article |
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