Fast Analysis of Superoxide Dismutase (SOD) Activity in Barley Leaves Using Visible and Near Infrared Spectroscopy
Visible and near infrared (Vis/NIR) spectroscopy was investigated for the fast analysis of superoxide dismutase (SOD) activity in barley (<em>Hordeum</em> <em>vulgare</em> L.) leaves. Seven different spectra preprocessing methods were compared....
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| Format: | Article |
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MDPI AG
2012-08-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/12/8/10871 |
| _version_ | 1818015506055036928 |
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| author | Fei Liu Yun Zhao Wenwen Kong Yong He Tian Tian Weijun Zhou |
| author_facet | Fei Liu Yun Zhao Wenwen Kong Yong He Tian Tian Weijun Zhou |
| author_sort | Fei Liu |
| collection | DOAJ |
| description | Visible and near infrared (Vis/NIR) spectroscopy was investigated for the fast analysis of superoxide dismutase (SOD) activity in barley (<em>Hordeum</em> <em>vulgare</em> L.) leaves. Seven different spectra preprocessing methods were compared. Four regression methods were used for comparison of prediction performance, including partial least squares (PLS), multiple linear regression (MLR), least squares-support vector machine (LS-SVM) and Gaussian process regress (GPR). Successive projections algorithm (SPA) and regression coefficients (RC) were applied to select effective wavelengths (EWs) to develop more parsimonious models. The results indicated that Savitzky-Golay smoothing (SG) and multiplicative scatter correction (MSC) should be selected as the optimum preprocessing methods. The best prediction performance was achieved by the LV-LS-SVM model on SG spectra, and the correlation coefficients (<em>r</em>) and root mean square error of prediction (RMSEP) were 0.9064 and 0.5336, respectively. The conclusion was that Vis/NIR spectroscopy combined with multivariate analysis could be successfully applied for the fast estimation of SOD activity in barley leaves. |
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| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T06:58:57Z |
| publishDate | 2012-08-01 |
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| spelling | doaj.art-5693f8ef80e541eaa2862d288eeb36422022-12-22T02:06:49ZengMDPI AGSensors1424-82202012-08-01128108711088010.3390/s120810871Fast Analysis of Superoxide Dismutase (SOD) Activity in Barley Leaves Using Visible and Near Infrared SpectroscopyFei LiuYun ZhaoWenwen KongYong HeTian TianWeijun ZhouVisible and near infrared (Vis/NIR) spectroscopy was investigated for the fast analysis of superoxide dismutase (SOD) activity in barley (<em>Hordeum</em> <em>vulgare</em> L.) leaves. Seven different spectra preprocessing methods were compared. Four regression methods were used for comparison of prediction performance, including partial least squares (PLS), multiple linear regression (MLR), least squares-support vector machine (LS-SVM) and Gaussian process regress (GPR). Successive projections algorithm (SPA) and regression coefficients (RC) were applied to select effective wavelengths (EWs) to develop more parsimonious models. The results indicated that Savitzky-Golay smoothing (SG) and multiplicative scatter correction (MSC) should be selected as the optimum preprocessing methods. The best prediction performance was achieved by the LV-LS-SVM model on SG spectra, and the correlation coefficients (<em>r</em>) and root mean square error of prediction (RMSEP) were 0.9064 and 0.5336, respectively. The conclusion was that Vis/NIR spectroscopy combined with multivariate analysis could be successfully applied for the fast estimation of SOD activity in barley leaves.http://www.mdpi.com/1424-8220/12/8/10871visible and near infrared spectroscopybarleysuperoxide dismutasevariable selectionleast squares-support vector machineGaussian process regression |
| spellingShingle | Fei Liu Yun Zhao Wenwen Kong Yong He Tian Tian Weijun Zhou Fast Analysis of Superoxide Dismutase (SOD) Activity in Barley Leaves Using Visible and Near Infrared Spectroscopy Sensors visible and near infrared spectroscopy barley superoxide dismutase variable selection least squares-support vector machine Gaussian process regression |
| title | Fast Analysis of Superoxide Dismutase (SOD) Activity in Barley Leaves Using Visible and Near Infrared Spectroscopy |
| title_full | Fast Analysis of Superoxide Dismutase (SOD) Activity in Barley Leaves Using Visible and Near Infrared Spectroscopy |
| title_fullStr | Fast Analysis of Superoxide Dismutase (SOD) Activity in Barley Leaves Using Visible and Near Infrared Spectroscopy |
| title_full_unstemmed | Fast Analysis of Superoxide Dismutase (SOD) Activity in Barley Leaves Using Visible and Near Infrared Spectroscopy |
| title_short | Fast Analysis of Superoxide Dismutase (SOD) Activity in Barley Leaves Using Visible and Near Infrared Spectroscopy |
| title_sort | fast analysis of superoxide dismutase sod activity in barley leaves using visible and near infrared spectroscopy |
| topic | visible and near infrared spectroscopy barley superoxide dismutase variable selection least squares-support vector machine Gaussian process regression |
| url | http://www.mdpi.com/1424-8220/12/8/10871 |
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