Optical Mapping of Cardiomyocytes in Monolayer Derived from Induced Pluripotent Stem Cells
Optical mapping is a powerful imaging technique widely adopted to measure membrane potential changes and intracellular Ca<sup>2+</sup> variations in excitable tissues using voltage-sensitive dyes and Ca<sup>2+</sup> indicators, respectively. This powerful tool has rapidly bec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/17/2168 |
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| author | Mohammed Djemai Michael Cupelli Mohamed Boutjdir Mohamed Chahine |
| author_facet | Mohammed Djemai Michael Cupelli Mohamed Boutjdir Mohamed Chahine |
| author_sort | Mohammed Djemai |
| collection | DOAJ |
| description | Optical mapping is a powerful imaging technique widely adopted to measure membrane potential changes and intracellular Ca<sup>2+</sup> variations in excitable tissues using voltage-sensitive dyes and Ca<sup>2+</sup> indicators, respectively. This powerful tool has rapidly become indispensable in the field of cardiac electrophysiology for studying depolarization wave propagation, estimating the conduction velocity of electrical impulses, and measuring Ca<sup>2+</sup> dynamics in cardiac cells and tissues. In addition, mapping these electrophysiological parameters is important for understanding cardiac arrhythmia mechanisms. In this review, we delve into the fundamentals of cardiac optical mapping technology and its applications when applied to hiPSC-derived cardiomyocytes and discuss related advantages and challenges. We also provide a detailed description of the processing and analysis of optical mapping data, which is a crucial step in the study of cardiac diseases and arrhythmia mechanisms for extracting and comparing relevant electrophysiological parameters. |
| first_indexed | 2024-03-10T23:25:39Z |
| format | Article |
| id | doaj.art-5695b73a665f43a68290edd854724c52 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T23:25:39Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-5695b73a665f43a68290edd854724c522023-11-19T07:58:11ZengMDPI AGCells2073-44092023-08-011217216810.3390/cells12172168Optical Mapping of Cardiomyocytes in Monolayer Derived from Induced Pluripotent Stem CellsMohammed Djemai0Michael Cupelli1Mohamed Boutjdir2Mohamed Chahine3CERVO Brain Research Center, Institut Universitaire en Santé Mentale de Québec, Quebec City, QC G1J 2G3, CanadaCardiovascular Research Program, VA New York Harbor Healthcare System, New York, NY 11209, USACardiovascular Research Program, VA New York Harbor Healthcare System, New York, NY 11209, USACERVO Brain Research Center, Institut Universitaire en Santé Mentale de Québec, Quebec City, QC G1J 2G3, CanadaOptical mapping is a powerful imaging technique widely adopted to measure membrane potential changes and intracellular Ca<sup>2+</sup> variations in excitable tissues using voltage-sensitive dyes and Ca<sup>2+</sup> indicators, respectively. This powerful tool has rapidly become indispensable in the field of cardiac electrophysiology for studying depolarization wave propagation, estimating the conduction velocity of electrical impulses, and measuring Ca<sup>2+</sup> dynamics in cardiac cells and tissues. In addition, mapping these electrophysiological parameters is important for understanding cardiac arrhythmia mechanisms. In this review, we delve into the fundamentals of cardiac optical mapping technology and its applications when applied to hiPSC-derived cardiomyocytes and discuss related advantages and challenges. We also provide a detailed description of the processing and analysis of optical mapping data, which is a crucial step in the study of cardiac diseases and arrhythmia mechanisms for extracting and comparing relevant electrophysiological parameters.https://www.mdpi.com/2073-4409/12/17/2168optical mappingcardiac electrophysiologymembrane potentialsCa<sup>2+</sup> imagingconduction velocityiPS cells |
| spellingShingle | Mohammed Djemai Michael Cupelli Mohamed Boutjdir Mohamed Chahine Optical Mapping of Cardiomyocytes in Monolayer Derived from Induced Pluripotent Stem Cells Cells optical mapping cardiac electrophysiology membrane potentials Ca<sup>2+</sup> imaging conduction velocity iPS cells |
| title | Optical Mapping of Cardiomyocytes in Monolayer Derived from Induced Pluripotent Stem Cells |
| title_full | Optical Mapping of Cardiomyocytes in Monolayer Derived from Induced Pluripotent Stem Cells |
| title_fullStr | Optical Mapping of Cardiomyocytes in Monolayer Derived from Induced Pluripotent Stem Cells |
| title_full_unstemmed | Optical Mapping of Cardiomyocytes in Monolayer Derived from Induced Pluripotent Stem Cells |
| title_short | Optical Mapping of Cardiomyocytes in Monolayer Derived from Induced Pluripotent Stem Cells |
| title_sort | optical mapping of cardiomyocytes in monolayer derived from induced pluripotent stem cells |
| topic | optical mapping cardiac electrophysiology membrane potentials Ca<sup>2+</sup> imaging conduction velocity iPS cells |
| url | https://www.mdpi.com/2073-4409/12/17/2168 |
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