Metabolic engineering of plant secondary metabolites: prospects and its technological challenges
Plants produce a wide range of secondary metabolites that play vital roles for their primary functions such as growth, defence, adaptations or reproduction. Some of the plant secondary metabolites are beneficial to mankind as nutraceuticals and pharmaceuticals. Metabolic pathways and their regulator...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-05-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1171154/full |
| _version_ | 1797828594255265792 |
|---|---|
| author | Asem Mipeshwaree Devi Khomdram Khedashwori Devi Pukhrambam Premi Devi Moirangthem Lakshmipriyari Devi Sudripta Das |
| author_facet | Asem Mipeshwaree Devi Khomdram Khedashwori Devi Pukhrambam Premi Devi Moirangthem Lakshmipriyari Devi Sudripta Das |
| author_sort | Asem Mipeshwaree Devi |
| collection | DOAJ |
| description | Plants produce a wide range of secondary metabolites that play vital roles for their primary functions such as growth, defence, adaptations or reproduction. Some of the plant secondary metabolites are beneficial to mankind as nutraceuticals and pharmaceuticals. Metabolic pathways and their regulatory mechanism are crucial for targeting metabolite engineering. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-mediated system has been widely applied in genome editing with high accuracy, efficiency, and multiplex targeting ability. Besides its vast application in genetic improvement, the technique also facilitates a comprehensive profiling approach to functional genomics related to gene discovery involved in various plant secondary metabolic pathways. Despite these wide applications, several challenges limit CRISPR/Cas system applicability in genome editing in plants. This review highlights updated applications of CRISPR/Cas system-mediated metabolic engineering of plants and its challenges. |
| first_indexed | 2024-04-09T13:06:49Z |
| format | Article |
| id | doaj.art-5696a73f95d140fabb3ac5ac5144389b |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T13:06:49Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-5696a73f95d140fabb3ac5ac5144389b2023-05-12T13:28:22ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-05-011410.3389/fpls.2023.11711541171154Metabolic engineering of plant secondary metabolites: prospects and its technological challengesAsem Mipeshwaree DeviKhomdram Khedashwori DeviPukhrambam Premi DeviMoirangthem Lakshmipriyari DeviSudripta DasPlants produce a wide range of secondary metabolites that play vital roles for their primary functions such as growth, defence, adaptations or reproduction. Some of the plant secondary metabolites are beneficial to mankind as nutraceuticals and pharmaceuticals. Metabolic pathways and their regulatory mechanism are crucial for targeting metabolite engineering. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-mediated system has been widely applied in genome editing with high accuracy, efficiency, and multiplex targeting ability. Besides its vast application in genetic improvement, the technique also facilitates a comprehensive profiling approach to functional genomics related to gene discovery involved in various plant secondary metabolic pathways. Despite these wide applications, several challenges limit CRISPR/Cas system applicability in genome editing in plants. This review highlights updated applications of CRISPR/Cas system-mediated metabolic engineering of plants and its challenges.https://www.frontiersin.org/articles/10.3389/fpls.2023.1171154/fullCRISPR/Cas9secondary metabolismflavonoidsmetabolite engineeringprime editing guide RNA |
| spellingShingle | Asem Mipeshwaree Devi Khomdram Khedashwori Devi Pukhrambam Premi Devi Moirangthem Lakshmipriyari Devi Sudripta Das Metabolic engineering of plant secondary metabolites: prospects and its technological challenges Frontiers in Plant Science CRISPR/Cas9 secondary metabolism flavonoids metabolite engineering prime editing guide RNA |
| title | Metabolic engineering of plant secondary metabolites: prospects and its technological challenges |
| title_full | Metabolic engineering of plant secondary metabolites: prospects and its technological challenges |
| title_fullStr | Metabolic engineering of plant secondary metabolites: prospects and its technological challenges |
| title_full_unstemmed | Metabolic engineering of plant secondary metabolites: prospects and its technological challenges |
| title_short | Metabolic engineering of plant secondary metabolites: prospects and its technological challenges |
| title_sort | metabolic engineering of plant secondary metabolites prospects and its technological challenges |
| topic | CRISPR/Cas9 secondary metabolism flavonoids metabolite engineering prime editing guide RNA |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1171154/full |
| work_keys_str_mv | AT asemmipeshwareedevi metabolicengineeringofplantsecondarymetabolitesprospectsanditstechnologicalchallenges AT khomdramkhedashworidevi metabolicengineeringofplantsecondarymetabolitesprospectsanditstechnologicalchallenges AT pukhrambampremidevi metabolicengineeringofplantsecondarymetabolitesprospectsanditstechnologicalchallenges AT moirangthemlakshmipriyaridevi metabolicengineeringofplantsecondarymetabolitesprospectsanditstechnologicalchallenges AT sudriptadas metabolicengineeringofplantsecondarymetabolitesprospectsanditstechnologicalchallenges |