Production and optimisation of hyaluronic acid extracted from Streptococcus pyogenes
Objectives Because of the less studies in this field in Iraq, this study aimed to use local Streptococcus pyogenes isolates to produce hyluronic acid. Methods The quantitative estimation of hyaluronic acid (HA) produced from eight local S. pyogenes isolates at different pH (6.3, 6.6, 6.9, 7.2, and 7...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nab'a Al-Hayat Foundation for Medical Sciences and Health Care - Press
2016-01-01
|
| Series: | Journal of Contemporary Medical Sciences |
| Subjects: | |
| Online Access: | http://jocms.org/index.php/jcms/article/view/52 |
| _version_ | 1819045256143831040 |
|---|---|
| author | Kawkab Abdulla Al-Saadiaa Hassan Fadhil Naji Ali Hmood Al-Saadi |
| author_facet | Kawkab Abdulla Al-Saadiaa Hassan Fadhil Naji Ali Hmood Al-Saadi |
| author_sort | Kawkab Abdulla Al-Saadiaa |
| collection | DOAJ |
| description | Objectives Because of the less studies in this field in Iraq, this study aimed to use local Streptococcus pyogenes isolates to produce hyluronic acid.
Methods The quantitative estimation of hyaluronic acid (HA) produced from eight local S. pyogenes isolates at different pH (6.3, 6.6, 6.9, 7.2,
and 7.5) and glucose concentration (4%, 6%, 8%, and 1%) were done using the HA ELISA kit.
Results This study showed that the maximum yield of HA was obtained at pH 7.5, and it was found that the differences in pH of the
production media of HA enhanced the HA production, but the glucose concentration has no beneficial effect for HA production.
Conclusion There were differences in HA production among local isolates at the same pH. |
| first_indexed | 2024-12-21T10:25:41Z |
| format | Article |
| id | doaj.art-5696b6193b8446a789c9c421a63a5c6d |
| institution | Directory Open Access Journal |
| issn | 2413-0516 |
| language | English |
| last_indexed | 2024-12-21T10:25:41Z |
| publishDate | 2016-01-01 |
| publisher | Nab'a Al-Hayat Foundation for Medical Sciences and Health Care - Press |
| record_format | Article |
| series | Journal of Contemporary Medical Sciences |
| spelling | doaj.art-5696b6193b8446a789c9c421a63a5c6d2022-12-21T19:07:19ZengNab'a Al-Hayat Foundation for Medical Sciences and Health Care - PressJournal of Contemporary Medical Sciences2413-05162016-01-0114131540Production and optimisation of hyaluronic acid extracted from Streptococcus pyogenesKawkab Abdulla Al-Saadiaa0Hassan Fadhil Naji1Ali Hmood Al-Saadi2University of Karbala, College of Science, Department of BiologyUniversity of Babylon, College of Science, Department of BiologyUniversity of Babylon, College of Science, Department of BiologyObjectives Because of the less studies in this field in Iraq, this study aimed to use local Streptococcus pyogenes isolates to produce hyluronic acid. Methods The quantitative estimation of hyaluronic acid (HA) produced from eight local S. pyogenes isolates at different pH (6.3, 6.6, 6.9, 7.2, and 7.5) and glucose concentration (4%, 6%, 8%, and 1%) were done using the HA ELISA kit. Results This study showed that the maximum yield of HA was obtained at pH 7.5, and it was found that the differences in pH of the production media of HA enhanced the HA production, but the glucose concentration has no beneficial effect for HA production. Conclusion There were differences in HA production among local isolates at the same pH.http://jocms.org/index.php/jcms/article/view/52Streptococcus pyogenes, hyaluronic acid, HA ELISA kit, pH, glucose concentration |
| spellingShingle | Kawkab Abdulla Al-Saadiaa Hassan Fadhil Naji Ali Hmood Al-Saadi Production and optimisation of hyaluronic acid extracted from Streptococcus pyogenes Journal of Contemporary Medical Sciences Streptococcus pyogenes, hyaluronic acid, HA ELISA kit, pH, glucose concentration |
| title | Production and optimisation of hyaluronic acid extracted from Streptococcus pyogenes |
| title_full | Production and optimisation of hyaluronic acid extracted from Streptococcus pyogenes |
| title_fullStr | Production and optimisation of hyaluronic acid extracted from Streptococcus pyogenes |
| title_full_unstemmed | Production and optimisation of hyaluronic acid extracted from Streptococcus pyogenes |
| title_short | Production and optimisation of hyaluronic acid extracted from Streptococcus pyogenes |
| title_sort | production and optimisation of hyaluronic acid extracted from streptococcus pyogenes |
| topic | Streptococcus pyogenes, hyaluronic acid, HA ELISA kit, pH, glucose concentration |
| url | http://jocms.org/index.php/jcms/article/view/52 |
| work_keys_str_mv | AT kawkababdullaalsaadiaa productionandoptimisationofhyaluronicacidextractedfromstreptococcuspyogenes AT hassanfadhilnaji productionandoptimisationofhyaluronicacidextractedfromstreptococcuspyogenes AT alihmoodalsaadi productionandoptimisationofhyaluronicacidextractedfromstreptococcuspyogenes |