Synoviolin alleviates GSDMD‐mediated periodontitis by suppressing its stability
Abstract Introduction Inflammasome and pyroptosis play important roles in periodontitis. Gasdermin D (GSDMD), a key factor in pyroptosis, is cleaved by caspase‐1 and regulated by ubiquitination. Synoviolin is a ubiquitin E3 ligase that interacts with GSDMD. In this study, the effects of Synoviolin o...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-07-01
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| Series: | Immunity, Inflammation and Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/iid3.880 |
| _version_ | 1797770330355269632 |
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| author | Ying Pang Lili Liu Shuainan Wu Jianqi Wang Lu Liu |
| author_facet | Ying Pang Lili Liu Shuainan Wu Jianqi Wang Lu Liu |
| author_sort | Ying Pang |
| collection | DOAJ |
| description | Abstract Introduction Inflammasome and pyroptosis play important roles in periodontitis. Gasdermin D (GSDMD), a key factor in pyroptosis, is cleaved by caspase‐1 and regulated by ubiquitination. Synoviolin is a ubiquitin E3 ligase that interacts with GSDMD. In this study, the effects of Synoviolin on inflammasome activation and periodontitis were explored. Methods The expression of IL‐1β, GSDMD, and Synoviolin in peripheral blood mononuclear cells from patients with periodontitis was determined. The interaction between GSDMD and Synoviolin was studied. The cytokine level in gingival tissues and the distance from the cementoenamel junction to the alveolar bone crest were measured in mice with Synoviolin deficiency in myeloid cells. Results We reported that elevated mRNA and protein levels of IL‐1β and GSDMD, decreased levels of Synoviolin mRNA and protein, and decreased ubiquitination of GSDMD were associated with periodontitis. Synoviolin interacted with GSDMD. Synoviolin‐deficient bone marrow‐derived macrophages had increased IL‐1β and IL‐18 secretion after ATP stimulation. Mice with Synoviolin deficiency in myeloid cells had more severe periodontitis and upregulated IL‐1β and IL‐18. Conclusions Therefore, we conclude that Synoviolin suppresses inflammasome activation and periodontitis by regulating GSDMD stability. |
| first_indexed | 2024-03-12T21:21:43Z |
| format | Article |
| id | doaj.art-5697a30aad5e488fa53108a54db8f0c9 |
| institution | Directory Open Access Journal |
| issn | 2050-4527 |
| language | English |
| last_indexed | 2024-03-12T21:21:43Z |
| publishDate | 2023-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj.art-5697a30aad5e488fa53108a54db8f0c92023-07-28T17:22:31ZengWileyImmunity, Inflammation and Disease2050-45272023-07-01117n/an/a10.1002/iid3.880Synoviolin alleviates GSDMD‐mediated periodontitis by suppressing its stabilityYing Pang0Lili Liu1Shuainan Wu2Jianqi Wang3Lu Liu4Dental Clinic, Cangzhou Central Hospital Cangzhou Hebei People's Republic of ChinaDental Clinic, Cangzhou Central Hospital Cangzhou Hebei People's Republic of ChinaDental Clinic, Cangzhou Central Hospital Cangzhou Hebei People's Republic of ChinaDental Clinic, Cangzhou Central Hospital Cangzhou Hebei People's Republic of ChinaDental Clinic, Cangzhou Central Hospital Cangzhou Hebei People's Republic of ChinaAbstract Introduction Inflammasome and pyroptosis play important roles in periodontitis. Gasdermin D (GSDMD), a key factor in pyroptosis, is cleaved by caspase‐1 and regulated by ubiquitination. Synoviolin is a ubiquitin E3 ligase that interacts with GSDMD. In this study, the effects of Synoviolin on inflammasome activation and periodontitis were explored. Methods The expression of IL‐1β, GSDMD, and Synoviolin in peripheral blood mononuclear cells from patients with periodontitis was determined. The interaction between GSDMD and Synoviolin was studied. The cytokine level in gingival tissues and the distance from the cementoenamel junction to the alveolar bone crest were measured in mice with Synoviolin deficiency in myeloid cells. Results We reported that elevated mRNA and protein levels of IL‐1β and GSDMD, decreased levels of Synoviolin mRNA and protein, and decreased ubiquitination of GSDMD were associated with periodontitis. Synoviolin interacted with GSDMD. Synoviolin‐deficient bone marrow‐derived macrophages had increased IL‐1β and IL‐18 secretion after ATP stimulation. Mice with Synoviolin deficiency in myeloid cells had more severe periodontitis and upregulated IL‐1β and IL‐18. Conclusions Therefore, we conclude that Synoviolin suppresses inflammasome activation and periodontitis by regulating GSDMD stability.https://doi.org/10.1002/iid3.880GSDMDinflammasomeperiodontitisSynoviolin |
| spellingShingle | Ying Pang Lili Liu Shuainan Wu Jianqi Wang Lu Liu Synoviolin alleviates GSDMD‐mediated periodontitis by suppressing its stability Immunity, Inflammation and Disease GSDMD inflammasome periodontitis Synoviolin |
| title | Synoviolin alleviates GSDMD‐mediated periodontitis by suppressing its stability |
| title_full | Synoviolin alleviates GSDMD‐mediated periodontitis by suppressing its stability |
| title_fullStr | Synoviolin alleviates GSDMD‐mediated periodontitis by suppressing its stability |
| title_full_unstemmed | Synoviolin alleviates GSDMD‐mediated periodontitis by suppressing its stability |
| title_short | Synoviolin alleviates GSDMD‐mediated periodontitis by suppressing its stability |
| title_sort | synoviolin alleviates gsdmd mediated periodontitis by suppressing its stability |
| topic | GSDMD inflammasome periodontitis Synoviolin |
| url | https://doi.org/10.1002/iid3.880 |
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