| Summary: | The newly isolated strain KIGAM252<sup>T</sup> was found to be facultatively anaerobic, Gram-stain-positive, spore-forming, and rod-shaped. They grew at 10–45 °C, pH 6.0–10.0, and were able to tolerate up to 6% NaCl in the growth medium. Phylogenetic analysis indicated that the KIGAM252<sup>T</sup> strain was related to the genus <i>Metabacillus</i>. The cell membrane fatty acid composition of strain KIGAM252<sup>T</sup> included C<sub>15:0</sub> anteiso and C<sub>15:0</sub> iso (25.6%) as the major fatty acids, and menaquinone 7 was the predominant isoprenoid quinone. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The size of the whole genome was 4.30 Mbp, and the G + C content of the DNA was 43.8%. Average nucleotide and amino acid identity and in silico DNA-DNA hybridization values were below the species delineation threshold. Pan-genomic analysis revealed that 15.8% of all genes present in strain KIGAM252<sup>T</sup> was unique to the strain. The analysis of the secondary biosynthetic pathway predicted the carotenoid synthetic gene cluster in the strain KIGAM252<sup>T</sup>. Based on these current polyphasic taxonomic data, strain KIGAM252<sup>T</sup> represents a novel species of the genus <i>Metabacillus</i> that produces carotenoids, for which we propose the name <i>Metabacillus flavus</i> sp. nov. The type of strain was KIGAM252<sup>T</sup> (=KCTC 43261<sup>T</sup> = JCM 34406<sup>T</sup>).
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