Rapid generation of long tandem DNA repeat arrays by homologous recombination in yeast to study their function in mammalian genomes
<p>Abstract</p> <p>We describe here a method to rapidly convert any desirable DNA fragment, as small as 100 bp, into long tandem DNA arrays up to 140 kb in size that are inserted into a microbe vector. This method includes rolling-circle phi29 amplification (RCA) of the sequence &l...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-10-01
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| Series: | Biological Procedures Online |
| Online Access: | http://www.biologicalproceduresonline.com/content/13/1/8 |