Rapid generation of long tandem DNA repeat arrays by homologous recombination in yeast to study their function in mammalian genomes

Rapid generation of long tandem DNA repeat arrays by homologous recombination in yeast to study their function in mammalian genomes

<p>Abstract</p> <p>We describe here a method to rapidly convert any desirable DNA fragment, as small as 100 bp, into long tandem DNA arrays up to 140 kb in size that are inserted into a microbe vector. This method includes rolling-circle phi29 amplification (RCA) of the sequence &l...

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Bibliographic Details
Main Authors: Kouprina Natalay, Larionov Vladimir, Lee Nicholas CO, Noskov Vladimir N
Format: Article
Language:English
Published: BMC 2011-10-01
Series:Biological Procedures Online
Online Access:http://www.biologicalproceduresonline.com/content/13/1/8

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