Biochemical Characterization of Parsley Glycosyltransferases Involved in the Biosynthesis of a Flavonoid Glycoside, Apiin
The flavonoid glycoside apiin (apigenin 7-<i>O</i>-[β-D-apiosyl-(1→2)-β-D-glucoside]) is abundant in apiaceous and asteraceous plants, including celery and parsley. Although several enzymes involved in apiin biosynthesis have been identified in celery, many of the enzymes in parsley (<...
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/23/17118 |
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| author | Song An Maho Yamashita Sho Iguchi Taketo Kihara Eri Kamon Kazuya Ishikawa Masaru Kobayashi Takeshi Ishimizu |
| author_facet | Song An Maho Yamashita Sho Iguchi Taketo Kihara Eri Kamon Kazuya Ishikawa Masaru Kobayashi Takeshi Ishimizu |
| author_sort | Song An |
| collection | DOAJ |
| description | The flavonoid glycoside apiin (apigenin 7-<i>O</i>-[β-D-apiosyl-(1→2)-β-D-glucoside]) is abundant in apiaceous and asteraceous plants, including celery and parsley. Although several enzymes involved in apiin biosynthesis have been identified in celery, many of the enzymes in parsley (<i>Petroselinum crispum</i>) have not been identified. In this study, we identified parsley genes encoding the glucosyltransferase, PcGlcT, and the apiosyltransferase, PcApiT, that catalyze the glycosylation steps of apiin biosynthesis. Their substrate specificities showed that they were involved in the biosynthesis of some flavonoid 7-<i>O</i>-apiosylglucosides, including apiin. The expression profiles of <i>PcGlcT</i> and <i>PcApiT</i> were closely correlated with the accumulation of flavonoid 7-<i>O</i>-apiosylglucosides in parsley organs and developmental stages. These findings support the idea that <i>PcGlcT</i> and <i>PcApiT</i> are involved in the biosynthesis of flavonoid 7-<i>O</i>-apiosylglucosides in parsley. The identification of these genes will elucidate the physiological significance of apiin and the development of apiin production methods. |
| first_indexed | 2024-03-09T01:48:50Z |
| format | Article |
| id | doaj.art-569def8e634e426583601e27972d3ea6 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T01:48:50Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-569def8e634e426583601e27972d3ea62023-12-08T15:18:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124231711810.3390/ijms242317118Biochemical Characterization of Parsley Glycosyltransferases Involved in the Biosynthesis of a Flavonoid Glycoside, ApiinSong An0Maho Yamashita1Sho Iguchi2Taketo Kihara3Eri Kamon4Kazuya Ishikawa5Masaru Kobayashi6Takeshi Ishimizu7College of Life Sciences, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu 525-8577, Shiga, JapanCollege of Life Sciences, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu 525-8577, Shiga, JapanCollege of Life Sciences, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu 525-8577, Shiga, JapanCollege of Life Sciences, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu 525-8577, Shiga, JapanCollege of Life Sciences, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu 525-8577, Shiga, JapanCollege of Life Sciences, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu 525-8577, Shiga, JapanGraduate School of Agriculture, Kyoto University, Kyoto 606-8502, Kyoto, JapanCollege of Life Sciences, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu 525-8577, Shiga, JapanThe flavonoid glycoside apiin (apigenin 7-<i>O</i>-[β-D-apiosyl-(1→2)-β-D-glucoside]) is abundant in apiaceous and asteraceous plants, including celery and parsley. Although several enzymes involved in apiin biosynthesis have been identified in celery, many of the enzymes in parsley (<i>Petroselinum crispum</i>) have not been identified. In this study, we identified parsley genes encoding the glucosyltransferase, PcGlcT, and the apiosyltransferase, PcApiT, that catalyze the glycosylation steps of apiin biosynthesis. Their substrate specificities showed that they were involved in the biosynthesis of some flavonoid 7-<i>O</i>-apiosylglucosides, including apiin. The expression profiles of <i>PcGlcT</i> and <i>PcApiT</i> were closely correlated with the accumulation of flavonoid 7-<i>O</i>-apiosylglucosides in parsley organs and developmental stages. These findings support the idea that <i>PcGlcT</i> and <i>PcApiT</i> are involved in the biosynthesis of flavonoid 7-<i>O</i>-apiosylglucosides in parsley. The identification of these genes will elucidate the physiological significance of apiin and the development of apiin production methods.https://www.mdpi.com/1422-0067/24/23/17118apiinflavonoid glycosideglycosyltransferaseparsleyspecialized metabolite |
| spellingShingle | Song An Maho Yamashita Sho Iguchi Taketo Kihara Eri Kamon Kazuya Ishikawa Masaru Kobayashi Takeshi Ishimizu Biochemical Characterization of Parsley Glycosyltransferases Involved in the Biosynthesis of a Flavonoid Glycoside, Apiin International Journal of Molecular Sciences apiin flavonoid glycoside glycosyltransferase parsley specialized metabolite |
| title | Biochemical Characterization of Parsley Glycosyltransferases Involved in the Biosynthesis of a Flavonoid Glycoside, Apiin |
| title_full | Biochemical Characterization of Parsley Glycosyltransferases Involved in the Biosynthesis of a Flavonoid Glycoside, Apiin |
| title_fullStr | Biochemical Characterization of Parsley Glycosyltransferases Involved in the Biosynthesis of a Flavonoid Glycoside, Apiin |
| title_full_unstemmed | Biochemical Characterization of Parsley Glycosyltransferases Involved in the Biosynthesis of a Flavonoid Glycoside, Apiin |
| title_short | Biochemical Characterization of Parsley Glycosyltransferases Involved in the Biosynthesis of a Flavonoid Glycoside, Apiin |
| title_sort | biochemical characterization of parsley glycosyltransferases involved in the biosynthesis of a flavonoid glycoside apiin |
| topic | apiin flavonoid glycoside glycosyltransferase parsley specialized metabolite |
| url | https://www.mdpi.com/1422-0067/24/23/17118 |
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