Utilizing chemically induced dimerization of FKBP to analyze endocytosis by live-cell imaging in budding yeast
Summary: Chemically induced dimerization (CID) is a useful tool for artificially inducing protein-protein interactions. Although CID has been used extensively for live-cell microscopy applications in mammalian systems, it is rarely utilized in yeast cell biology studies. Here, we present a step-by-s...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-06-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722002039 |
| _version_ | 1818495059341869056 |
|---|---|
| author | Andrew K. Lamb Santiago M. Di Pietro |
| author_facet | Andrew K. Lamb Santiago M. Di Pietro |
| author_sort | Andrew K. Lamb |
| collection | DOAJ |
| description | Summary: Chemically induced dimerization (CID) is a useful tool for artificially inducing protein-protein interactions. Although CID has been used extensively for live-cell microscopy applications in mammalian systems, it is rarely utilized in yeast cell biology studies. Here, we present a step-by-step protocol for the utilization of a CID system in live-cell microscopy experiments of budding yeast endocytosis. While focusing on the study of endocytosis, this protocol framework is adaptable to the study of other cellular processes in Saccharomyces cerevisiae.For complete details on the use and execution of this protocol, please refer to Lamb et al. (2021). |
| first_indexed | 2024-12-10T18:15:13Z |
| format | Article |
| id | doaj.art-56a679cdc0a94401b7aefb1348e5eae9 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-10T18:15:13Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-56a679cdc0a94401b7aefb1348e5eae92022-12-22T01:38:20ZengElsevierSTAR Protocols2666-16672022-06-0132101323Utilizing chemically induced dimerization of FKBP to analyze endocytosis by live-cell imaging in budding yeastAndrew K. Lamb0Santiago M. Di Pietro1Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523-1870, USA; Corresponding authorDepartment of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523-1870, USA; Corresponding authorSummary: Chemically induced dimerization (CID) is a useful tool for artificially inducing protein-protein interactions. Although CID has been used extensively for live-cell microscopy applications in mammalian systems, it is rarely utilized in yeast cell biology studies. Here, we present a step-by-step protocol for the utilization of a CID system in live-cell microscopy experiments of budding yeast endocytosis. While focusing on the study of endocytosis, this protocol framework is adaptable to the study of other cellular processes in Saccharomyces cerevisiae.For complete details on the use and execution of this protocol, please refer to Lamb et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166722002039Cell BiologyCell-based AssaysMicroscopyModel OrganismsSignal Transduction |
| spellingShingle | Andrew K. Lamb Santiago M. Di Pietro Utilizing chemically induced dimerization of FKBP to analyze endocytosis by live-cell imaging in budding yeast STAR Protocols Cell Biology Cell-based Assays Microscopy Model Organisms Signal Transduction |
| title | Utilizing chemically induced dimerization of FKBP to analyze endocytosis by live-cell imaging in budding yeast |
| title_full | Utilizing chemically induced dimerization of FKBP to analyze endocytosis by live-cell imaging in budding yeast |
| title_fullStr | Utilizing chemically induced dimerization of FKBP to analyze endocytosis by live-cell imaging in budding yeast |
| title_full_unstemmed | Utilizing chemically induced dimerization of FKBP to analyze endocytosis by live-cell imaging in budding yeast |
| title_short | Utilizing chemically induced dimerization of FKBP to analyze endocytosis by live-cell imaging in budding yeast |
| title_sort | utilizing chemically induced dimerization of fkbp to analyze endocytosis by live cell imaging in budding yeast |
| topic | Cell Biology Cell-based Assays Microscopy Model Organisms Signal Transduction |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722002039 |
| work_keys_str_mv | AT andrewklamb utilizingchemicallyinduceddimerizationoffkbptoanalyzeendocytosisbylivecellimaginginbuddingyeast AT santiagomdipietro utilizingchemicallyinduceddimerizationoffkbptoanalyzeendocytosisbylivecellimaginginbuddingyeast |