Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm Culture
Outer membrane vesicles (OMV) derived from Bordetella pertussis—the etiologic agent of the resurgent disease called pertussis—are safe and effective in preventing bacterial colonization in the lungs of immunized mice. Vaccine formulations containing those OMV are capable of inducing a mixed Th1/Th2/...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-09-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.730434/full |
| _version_ | 1819083115743674368 |
|---|---|
| author | Francisco Carriquiriborde Pablo Martin Aispuro Nicolás Ambrosis Eugenia Zurita Daniela Bottero María Emilia Gaillard Celina Castuma Erika Rudi Aníbal Lodeiro Daniela F. Hozbor |
| author_facet | Francisco Carriquiriborde Pablo Martin Aispuro Nicolás Ambrosis Eugenia Zurita Daniela Bottero María Emilia Gaillard Celina Castuma Erika Rudi Aníbal Lodeiro Daniela F. Hozbor |
| author_sort | Francisco Carriquiriborde |
| collection | DOAJ |
| description | Outer membrane vesicles (OMV) derived from Bordetella pertussis—the etiologic agent of the resurgent disease called pertussis—are safe and effective in preventing bacterial colonization in the lungs of immunized mice. Vaccine formulations containing those OMV are capable of inducing a mixed Th1/Th2/Th17 profile, but even more interestingly, they may induce a tissue-resident memory immune response. This immune response is recommended for the new generation of pertussis-vaccines that must be developed to overcome the weaknesses of current commercial acellular vaccines (second-generation of pertussis vaccine). The third-generation of pertussis vaccine should also deal with infections caused by bacteria that currently circulate in the population and are phenotypically and genotypically different [in particular those deficient in the expression of pertactin antigen, PRN(-)] from those that circulated in the past. Here we evaluated the protective capacity of OMV derived from bacteria grown in biofilm, since it was observed that, by difference with older culture collection vaccine strains, circulating clinical B. pertussis isolates possess higher capacity for this lifestyle. Therefore, we performed studies with a clinical isolate with good biofilm-forming capacity. Biofilm lifestyle was confirmed by both scanning electron microscopy and proteomics. While scanning electron microscopy revealed typical biofilm structures in these cultures, BipA, fimbria, and other adhesins described as typical of the biofilm lifestyle were overexpressed in the biofilm culture in comparison with planktonic culture. OMV derived from biofilm (OMVbiof) or planktonic lifestyle (OMVplank) were used to formulate vaccines to compare their immunogenicity and protective capacities against infection with PRN(+) or PRN(-) B. pertussis clinical isolates. Using the mouse protection model, we detected that OMVbiof-vaccine was more immunogenic than OMVplank-vaccine in terms of both specific antibody titers and quality, since OMVbiof-vaccine induced antibodies with higher avidity. Moreover, when OMV were administered at suboptimal quantity for protection, OMVbiof-vaccine exhibited a significantly adequate and higher protective capacity against PRN(+) or PRN(-) than OMVplank-vaccine. Our findings indicate that the vaccine based on B. pertussis biofilm-derived OMV induces high protection also against pertactin-deficient strains, with a robust immune response. |
| first_indexed | 2024-12-21T20:27:26Z |
| format | Article |
| id | doaj.art-56a78c714cdc45b8a9e8d1e580f7c438 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-21T20:27:26Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-56a78c714cdc45b8a9e8d1e580f7c4382022-12-21T18:51:21ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-09-011210.3389/fimmu.2021.730434730434Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm CultureFrancisco Carriquiriborde0Pablo Martin Aispuro1Nicolás Ambrosis2Eugenia Zurita3Daniela Bottero4María Emilia Gaillard5Celina Castuma6Erika Rudi7Aníbal Lodeiro8Daniela F. Hozbor9Laboratorio VacSal, Instituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-CONICET La Plata, La Plata, ArgentinaLaboratorio VacSal, Instituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-CONICET La Plata, La Plata, ArgentinaLaboratorio VacSal, Instituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-CONICET La Plata, La Plata, ArgentinaLaboratorio VacSal, Instituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-CONICET La Plata, La Plata, ArgentinaLaboratorio VacSal, Instituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-CONICET La Plata, La Plata, ArgentinaLaboratorio VacSal, Instituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-CONICET La Plata, La Plata, ArgentinaLaboratorio VacSal, Instituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-CONICET La Plata, La Plata, ArgentinaLaboratorio VacSal, Instituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-CONICET La Plata, La Plata, ArgentinaInstituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-CONICET La Plata, La Plata, ArgentinaLaboratorio VacSal, Instituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-CONICET La Plata, La Plata, ArgentinaOuter membrane vesicles (OMV) derived from Bordetella pertussis—the etiologic agent of the resurgent disease called pertussis—are safe and effective in preventing bacterial colonization in the lungs of immunized mice. Vaccine formulations containing those OMV are capable of inducing a mixed Th1/Th2/Th17 profile, but even more interestingly, they may induce a tissue-resident memory immune response. This immune response is recommended for the new generation of pertussis-vaccines that must be developed to overcome the weaknesses of current commercial acellular vaccines (second-generation of pertussis vaccine). The third-generation of pertussis vaccine should also deal with infections caused by bacteria that currently circulate in the population and are phenotypically and genotypically different [in particular those deficient in the expression of pertactin antigen, PRN(-)] from those that circulated in the past. Here we evaluated the protective capacity of OMV derived from bacteria grown in biofilm, since it was observed that, by difference with older culture collection vaccine strains, circulating clinical B. pertussis isolates possess higher capacity for this lifestyle. Therefore, we performed studies with a clinical isolate with good biofilm-forming capacity. Biofilm lifestyle was confirmed by both scanning electron microscopy and proteomics. While scanning electron microscopy revealed typical biofilm structures in these cultures, BipA, fimbria, and other adhesins described as typical of the biofilm lifestyle were overexpressed in the biofilm culture in comparison with planktonic culture. OMV derived from biofilm (OMVbiof) or planktonic lifestyle (OMVplank) were used to formulate vaccines to compare their immunogenicity and protective capacities against infection with PRN(+) or PRN(-) B. pertussis clinical isolates. Using the mouse protection model, we detected that OMVbiof-vaccine was more immunogenic than OMVplank-vaccine in terms of both specific antibody titers and quality, since OMVbiof-vaccine induced antibodies with higher avidity. Moreover, when OMV were administered at suboptimal quantity for protection, OMVbiof-vaccine exhibited a significantly adequate and higher protective capacity against PRN(+) or PRN(-) than OMVplank-vaccine. Our findings indicate that the vaccine based on B. pertussis biofilm-derived OMV induces high protection also against pertactin-deficient strains, with a robust immune response.https://www.frontiersin.org/articles/10.3389/fimmu.2021.730434/fullBordetella pertussisouter membrane vesiclesbiofilmplanktonicprotectionvaccine |
| spellingShingle | Francisco Carriquiriborde Pablo Martin Aispuro Nicolás Ambrosis Eugenia Zurita Daniela Bottero María Emilia Gaillard Celina Castuma Erika Rudi Aníbal Lodeiro Daniela F. Hozbor Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm Culture Frontiers in Immunology Bordetella pertussis outer membrane vesicles biofilm planktonic protection vaccine |
| title | Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm Culture |
| title_full | Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm Culture |
| title_fullStr | Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm Culture |
| title_full_unstemmed | Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm Culture |
| title_short | Pertussis Vaccine Candidate Based on Outer Membrane Vesicles Derived From Biofilm Culture |
| title_sort | pertussis vaccine candidate based on outer membrane vesicles derived from biofilm culture |
| topic | Bordetella pertussis outer membrane vesicles biofilm planktonic protection vaccine |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.730434/full |
| work_keys_str_mv | AT franciscocarriquiriborde pertussisvaccinecandidatebasedonoutermembranevesiclesderivedfrombiofilmculture AT pablomartinaispuro pertussisvaccinecandidatebasedonoutermembranevesiclesderivedfrombiofilmculture AT nicolasambrosis pertussisvaccinecandidatebasedonoutermembranevesiclesderivedfrombiofilmculture AT eugeniazurita pertussisvaccinecandidatebasedonoutermembranevesiclesderivedfrombiofilmculture AT danielabottero pertussisvaccinecandidatebasedonoutermembranevesiclesderivedfrombiofilmculture AT mariaemiliagaillard pertussisvaccinecandidatebasedonoutermembranevesiclesderivedfrombiofilmculture AT celinacastuma pertussisvaccinecandidatebasedonoutermembranevesiclesderivedfrombiofilmculture AT erikarudi pertussisvaccinecandidatebasedonoutermembranevesiclesderivedfrombiofilmculture AT aniballodeiro pertussisvaccinecandidatebasedonoutermembranevesiclesderivedfrombiofilmculture AT danielafhozbor pertussisvaccinecandidatebasedonoutermembranevesiclesderivedfrombiofilmculture |