Revealing proteins associated with symbiotic germination of Gastrodia elata by proteomic analysis
Abstract Background Gastrodia elata, a mycoheterotrophic orchid, is a well-known medicinal herb. In nature, the seed germination of G. elata requires proper fungal association, because of the absence of endosperm. To germinate successfully, G. elata obtains nutrition from mycorrhizal fungi such as M...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-03-01
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| Series: | Botanical Studies |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40529-018-0224-z |
| _version_ | 1818992744730722304 |
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| author | Xu Zeng Yuanyuan Li Hong Ling Juan Chen Shunxing Guo |
| author_facet | Xu Zeng Yuanyuan Li Hong Ling Juan Chen Shunxing Guo |
| author_sort | Xu Zeng |
| collection | DOAJ |
| description | Abstract Background Gastrodia elata, a mycoheterotrophic orchid, is a well-known medicinal herb. In nature, the seed germination of G. elata requires proper fungal association, because of the absence of endosperm. To germinate successfully, G. elata obtains nutrition from mycorrhizal fungi such as Mycena. However, Mycena is not able to supply nutrition for the further development and enlargement of protocorms into tubers, flowering and fruit setting of G. elata. To date, current genomic studies on this topic are limited. Here we used the proteomic approach to explore changes in G. elata at different stages of symbiotic germination. Results Using mass spectrometry, 3787 unique proteins were identified, of which 599 were classified as differentially accumulated proteins. Most of these differentially accumulated proteins were putatively involved in energy metabolism, plant defense, molecular signaling, and secondary metabolism. Among them, the defense genes (e.g., pathogenesis-/wound-related proteins, peroxidases, and serine/threonine-protein kinase) were highly expressed in late-stage protocorms, suggesting that fungal colonization triggered the significant defense responses of G. elata. Conclusions The present study indicated the metabolic change and defensive reaction could disrupt the balance between Mycena and G. elata during mycorrhizal symbiotic germination. |
| first_indexed | 2024-12-20T20:31:02Z |
| format | Article |
| id | doaj.art-56a8e74e4e2c40fda6cc0d4c0b6c4127 |
| institution | Directory Open Access Journal |
| issn | 1999-3110 |
| language | English |
| last_indexed | 2024-12-20T20:31:02Z |
| publishDate | 2018-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Botanical Studies |
| spelling | doaj.art-56a8e74e4e2c40fda6cc0d4c0b6c41272022-12-21T19:27:20ZengSpringerOpenBotanical Studies1999-31102018-03-015911710.1186/s40529-018-0224-zRevealing proteins associated with symbiotic germination of Gastrodia elata by proteomic analysisXu Zeng0Yuanyuan Li1Hong Ling2Juan Chen3Shunxing Guo4Institute of Medicinal Plant Development, Chinese Academy of Medical SciencesInstitute of Medicinal Plant Development, Chinese Academy of Medical SciencesInstitute of Medicinal Plant Development, Chinese Academy of Medical SciencesInstitute of Medicinal Plant Development, Chinese Academy of Medical SciencesInstitute of Medicinal Plant Development, Chinese Academy of Medical SciencesAbstract Background Gastrodia elata, a mycoheterotrophic orchid, is a well-known medicinal herb. In nature, the seed germination of G. elata requires proper fungal association, because of the absence of endosperm. To germinate successfully, G. elata obtains nutrition from mycorrhizal fungi such as Mycena. However, Mycena is not able to supply nutrition for the further development and enlargement of protocorms into tubers, flowering and fruit setting of G. elata. To date, current genomic studies on this topic are limited. Here we used the proteomic approach to explore changes in G. elata at different stages of symbiotic germination. Results Using mass spectrometry, 3787 unique proteins were identified, of which 599 were classified as differentially accumulated proteins. Most of these differentially accumulated proteins were putatively involved in energy metabolism, plant defense, molecular signaling, and secondary metabolism. Among them, the defense genes (e.g., pathogenesis-/wound-related proteins, peroxidases, and serine/threonine-protein kinase) were highly expressed in late-stage protocorms, suggesting that fungal colonization triggered the significant defense responses of G. elata. Conclusions The present study indicated the metabolic change and defensive reaction could disrupt the balance between Mycena and G. elata during mycorrhizal symbiotic germination.http://link.springer.com/article/10.1186/s40529-018-0224-zGastrodia elataMycorrhizal fungiSymbiotic germinationPlant defense |
| spellingShingle | Xu Zeng Yuanyuan Li Hong Ling Juan Chen Shunxing Guo Revealing proteins associated with symbiotic germination of Gastrodia elata by proteomic analysis Botanical Studies Gastrodia elata Mycorrhizal fungi Symbiotic germination Plant defense |
| title | Revealing proteins associated with symbiotic germination of Gastrodia elata by proteomic analysis |
| title_full | Revealing proteins associated with symbiotic germination of Gastrodia elata by proteomic analysis |
| title_fullStr | Revealing proteins associated with symbiotic germination of Gastrodia elata by proteomic analysis |
| title_full_unstemmed | Revealing proteins associated with symbiotic germination of Gastrodia elata by proteomic analysis |
| title_short | Revealing proteins associated with symbiotic germination of Gastrodia elata by proteomic analysis |
| title_sort | revealing proteins associated with symbiotic germination of gastrodia elata by proteomic analysis |
| topic | Gastrodia elata Mycorrhizal fungi Symbiotic germination Plant defense |
| url | http://link.springer.com/article/10.1186/s40529-018-0224-z |
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