A Bacterial Quorum Sensing Molecule Elicits a General Stress Response in Saccharomyces cerevisiae
Bacteria assess their population density through a chemical communication mechanism termed quorum sensing, in order to coordinate group behavior. Most research on quorum sensing has focused primarily on its role as an intraspecies chemical signaling mechanism that enables the regulation of certain p...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.632658/full |
| _version_ | 1819122610533826560 |
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| author | Antonia Delago Antonia Delago Rachel Gregor Rachel Gregor Luba Dubinsky Luba Dubinsky Rambabu Dandela Rambabu Dandela Adi Hendler Adi Hendler Pnina Krief Pnina Krief Josep Rayo Josep Rayo Amir Aharoni Amir Aharoni Michael M. Meijler Michael M. Meijler |
| author_facet | Antonia Delago Antonia Delago Rachel Gregor Rachel Gregor Luba Dubinsky Luba Dubinsky Rambabu Dandela Rambabu Dandela Adi Hendler Adi Hendler Pnina Krief Pnina Krief Josep Rayo Josep Rayo Amir Aharoni Amir Aharoni Michael M. Meijler Michael M. Meijler |
| author_sort | Antonia Delago |
| collection | DOAJ |
| description | Bacteria assess their population density through a chemical communication mechanism termed quorum sensing, in order to coordinate group behavior. Most research on quorum sensing has focused primarily on its role as an intraspecies chemical signaling mechanism that enables the regulation of certain phenotypes through targeted gene expression. However, in recent years several seminal studies have revealed important phenomena in which quorum sensing molecules appear to serve additional roles as interspecies signals that may regulate microbial ecology. In this study, we asked whether the budding yeast Saccharomyces cerevisiae can sense chemical signals from prokaryotes. When exposed to a variety of quorum sensing molecules from different bacterial species and from Candida albicans we found that N-(3-oxododecanoyl)-L-homoserine lactone (C12) from the opportunistic human pathogen Pseudomonas aeruginosa induces a remarkable stress response in yeast. Microarray experiments confirmed and aided in interpreting these findings, showing a unique and specific expression pattern that differed significantly from the response to previously described stress factors. We further characterized this response and report preliminary findings on the molecular basis for the recognition of C12 by the yeast. |
| first_indexed | 2024-12-22T06:55:12Z |
| format | Article |
| id | doaj.art-56aa3d3b4b64401688a253c35df5a221 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T06:55:12Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-56aa3d3b4b64401688a253c35df5a2212022-12-21T18:35:00ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-09-011210.3389/fmicb.2021.632658632658A Bacterial Quorum Sensing Molecule Elicits a General Stress Response in Saccharomyces cerevisiaeAntonia Delago0Antonia Delago1Rachel Gregor2Rachel Gregor3Luba Dubinsky4Luba Dubinsky5Rambabu Dandela6Rambabu Dandela7Adi Hendler8Adi Hendler9Pnina Krief10Pnina Krief11Josep Rayo12Josep Rayo13Amir Aharoni14Amir Aharoni15Michael M. Meijler16Michael M. Meijler17Department of Chemistry, Ben-Gurion University of the Negev, Be’er Sheva, IsraelThe National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be’er Sheva, IsraelDepartment of Chemistry, Ben-Gurion University of the Negev, Be’er Sheva, IsraelThe National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be’er Sheva, IsraelDepartment of Chemistry, Ben-Gurion University of the Negev, Be’er Sheva, IsraelThe National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be’er Sheva, IsraelDepartment of Chemistry, Ben-Gurion University of the Negev, Be’er Sheva, IsraelThe National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be’er Sheva, IsraelThe National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be’er Sheva, IsraelDepartment of Life Sciences, Ben-Gurion University of the Negev, Be’er Sheva, IsraelDepartment of Chemistry, Ben-Gurion University of the Negev, Be’er Sheva, IsraelThe National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be’er Sheva, IsraelDepartment of Chemistry, Ben-Gurion University of the Negev, Be’er Sheva, IsraelThe National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be’er Sheva, IsraelThe National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be’er Sheva, IsraelDepartment of Life Sciences, Ben-Gurion University of the Negev, Be’er Sheva, IsraelDepartment of Chemistry, Ben-Gurion University of the Negev, Be’er Sheva, IsraelThe National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be’er Sheva, IsraelBacteria assess their population density through a chemical communication mechanism termed quorum sensing, in order to coordinate group behavior. Most research on quorum sensing has focused primarily on its role as an intraspecies chemical signaling mechanism that enables the regulation of certain phenotypes through targeted gene expression. However, in recent years several seminal studies have revealed important phenomena in which quorum sensing molecules appear to serve additional roles as interspecies signals that may regulate microbial ecology. In this study, we asked whether the budding yeast Saccharomyces cerevisiae can sense chemical signals from prokaryotes. When exposed to a variety of quorum sensing molecules from different bacterial species and from Candida albicans we found that N-(3-oxododecanoyl)-L-homoserine lactone (C12) from the opportunistic human pathogen Pseudomonas aeruginosa induces a remarkable stress response in yeast. Microarray experiments confirmed and aided in interpreting these findings, showing a unique and specific expression pattern that differed significantly from the response to previously described stress factors. We further characterized this response and report preliminary findings on the molecular basis for the recognition of C12 by the yeast.https://www.frontiersin.org/articles/10.3389/fmicb.2021.632658/fullquorum sensinginterkingdom communicationSaccharomyces cerevisiaePseudomonas aeruginosastress responseMsn 2/4 |
| spellingShingle | Antonia Delago Antonia Delago Rachel Gregor Rachel Gregor Luba Dubinsky Luba Dubinsky Rambabu Dandela Rambabu Dandela Adi Hendler Adi Hendler Pnina Krief Pnina Krief Josep Rayo Josep Rayo Amir Aharoni Amir Aharoni Michael M. Meijler Michael M. Meijler A Bacterial Quorum Sensing Molecule Elicits a General Stress Response in Saccharomyces cerevisiae Frontiers in Microbiology quorum sensing interkingdom communication Saccharomyces cerevisiae Pseudomonas aeruginosa stress response Msn 2/4 |
| title | A Bacterial Quorum Sensing Molecule Elicits a General Stress Response in Saccharomyces cerevisiae |
| title_full | A Bacterial Quorum Sensing Molecule Elicits a General Stress Response in Saccharomyces cerevisiae |
| title_fullStr | A Bacterial Quorum Sensing Molecule Elicits a General Stress Response in Saccharomyces cerevisiae |
| title_full_unstemmed | A Bacterial Quorum Sensing Molecule Elicits a General Stress Response in Saccharomyces cerevisiae |
| title_short | A Bacterial Quorum Sensing Molecule Elicits a General Stress Response in Saccharomyces cerevisiae |
| title_sort | bacterial quorum sensing molecule elicits a general stress response in saccharomyces cerevisiae |
| topic | quorum sensing interkingdom communication Saccharomyces cerevisiae Pseudomonas aeruginosa stress response Msn 2/4 |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.632658/full |
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