Purification Methods and the Presence of RNA in Virus Particles and Extracellular Vesicles
The fields of extracellular vesicles (EV) and virus infections are marred in a debate on whether a particular mRNA or non-coding RNA (i.e., miRNA) is packaged into a virus particle or copurifying EV and similarly, whether a particular mRNA or non-coding RNA is contained in meaningful numbers within...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/9/917 |
| _version_ | 1797556505091768320 |
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| author | Yijun Zhou Ryan P. McNamara Dirk P. Dittmer |
| author_facet | Yijun Zhou Ryan P. McNamara Dirk P. Dittmer |
| author_sort | Yijun Zhou |
| collection | DOAJ |
| description | The fields of extracellular vesicles (EV) and virus infections are marred in a debate on whether a particular mRNA or non-coding RNA (i.e., miRNA) is packaged into a virus particle or copurifying EV and similarly, whether a particular mRNA or non-coding RNA is contained in meaningful numbers within an EV. Key in settling this debate, is whether the purification methods are adequate to separate virus particles, EV and contaminant soluble RNA and RNA:protein complexes. Differential centrifugation/ultracentrifugation and precipitating agents like polyethylene glycol are widely utilized for both EV and virus purifications. EV are known to co-sediment with virions and other particulates, such as defective interfering particles and protein aggregates. Here, we discuss how encased RNAs from a heterogeneous mixture of particles can be distinguished by different purification methods. This is particularly important for subsequent interpretation of whether the RNA associated phenotype is contributed solely by virus or EV particles or a mixture of both. We also discuss the discrepancy of miRNA abundance in EV from different input material. |
| first_indexed | 2024-03-10T17:03:52Z |
| format | Article |
| id | doaj.art-56adad20c9f94e6cb8a986b9572b79f5 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T17:03:52Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-56adad20c9f94e6cb8a986b9572b79f52023-11-20T10:52:59ZengMDPI AGViruses1999-49152020-08-0112991710.3390/v12090917Purification Methods and the Presence of RNA in Virus Particles and Extracellular VesiclesYijun Zhou0Ryan P. McNamara1Dirk P. Dittmer2Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC 27599, USALineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC 27599, USALineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC 27599, USAThe fields of extracellular vesicles (EV) and virus infections are marred in a debate on whether a particular mRNA or non-coding RNA (i.e., miRNA) is packaged into a virus particle or copurifying EV and similarly, whether a particular mRNA or non-coding RNA is contained in meaningful numbers within an EV. Key in settling this debate, is whether the purification methods are adequate to separate virus particles, EV and contaminant soluble RNA and RNA:protein complexes. Differential centrifugation/ultracentrifugation and precipitating agents like polyethylene glycol are widely utilized for both EV and virus purifications. EV are known to co-sediment with virions and other particulates, such as defective interfering particles and protein aggregates. Here, we discuss how encased RNAs from a heterogeneous mixture of particles can be distinguished by different purification methods. This is particularly important for subsequent interpretation of whether the RNA associated phenotype is contributed solely by virus or EV particles or a mixture of both. We also discuss the discrepancy of miRNA abundance in EV from different input material.https://www.mdpi.com/1999-4915/12/9/917virion RNAextracellular vesiclesexosomesherpesviruses |
| spellingShingle | Yijun Zhou Ryan P. McNamara Dirk P. Dittmer Purification Methods and the Presence of RNA in Virus Particles and Extracellular Vesicles Viruses virion RNA extracellular vesicles exosomes herpesviruses |
| title | Purification Methods and the Presence of RNA in Virus Particles and Extracellular Vesicles |
| title_full | Purification Methods and the Presence of RNA in Virus Particles and Extracellular Vesicles |
| title_fullStr | Purification Methods and the Presence of RNA in Virus Particles and Extracellular Vesicles |
| title_full_unstemmed | Purification Methods and the Presence of RNA in Virus Particles and Extracellular Vesicles |
| title_short | Purification Methods and the Presence of RNA in Virus Particles and Extracellular Vesicles |
| title_sort | purification methods and the presence of rna in virus particles and extracellular vesicles |
| topic | virion RNA extracellular vesicles exosomes herpesviruses |
| url | https://www.mdpi.com/1999-4915/12/9/917 |
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